Category Archives: health and safety

European Commission has issued evaluation of nanomaterial risk frameworks and tools

Despite complaints that there should have been more, there has been some research into risks where nanomaterials are concerned. While additional research would be welcome, it’s perhaps more imperative that standardized testing and risk frameworks are developed so, for example, carbon nanotube safety research in Japan can be compared with the similar research in the Netherlands, the US, and elsewhere. This March 15, 2017 news item on Nanowerk features some research analyzing risk assessment frameworks and tools in Europe,

A recent study has evaluated frameworks and tools used in Europe to assess the potential health and environmental risks of manufactured nanomaterials. The study identifies a trend towards tools that provide protocols for conducting experiments, which enable more flexible and efficient hazard testing. Among its conclusions, however, it notes that no existing frameworks meet all the study’s evaluation criteria and calls for a new, more comprehensive framework.

A March 9, 2017 news alert in the European Commission’s Science for Environment Policy series, which originated the news item, provides more detail (Note: Links have been removed),

Nanotechnology is identified as a key emerging technology in the EU’s growth strategy, Europe 2020. It has great potential to contribute to innovation and economic growth and many of its applications have already received large investments. However,there are some uncertainties surrounding the environmental, health and safety risks of manufactured nanomaterials. For effective regulation, careful scientific analysis of their potential impacts is needed, as conducted through risk assessment exercises.

This study, conducted under the EU-funded MARINA project1, reviewed existing frameworks and tools for risk assessing manufactured nanomaterials. The researchers define a framework as a ‘conceptual paradigm’ of how a risk assessment should be conducted and understood, and give the REACH chemical safety assessment as an example. Tools are defined as implements used to carry out a specific task or function, such as experimental protocols, computer models or databases.

In all, 12 frameworks and 48 tools were evaluated. These were identified from other studies and projects. The frameworks were assessed against eight criteria which represent different strengths, such as whether they consider properties specific to nanomaterials, whether they consider the entire life cycle of a nanomaterial and whether they include careful planning and prioritise objectives before the risk assessment is conducted.

The tools were assessed against seven criteria, such as ease of use, whether they provide quantitative information and if they clearly communicate uncertainty in their results. The researchers defined the criteria for both frameworks and tools by reviewing other studies and by interviewing staff at organisations who develop tools.

The evaluation was thus able to produce a list of strengths and areas for improvement for the frameworks and tools, based on whether they meet each of the criteria. Among its many findings, the evaluation showed that most of the frameworks stress that ‘problem formulation’, which sets the goals and scope of an assessment during the planning process, is essential to avoid unnecessary testing. In addition, most frameworks consider routes of exposure in the initial stages of assessment, which is beneficial as it can exclude irrelevant exposure routes and avoid unnecessary tests.

However, none of the frameworks met all eight of the criteria. The study therefore recommends that a new, comprehensive framework is developed that meets all criteria. Such a framework is needed to inform regulation, the researchers say, and should integrate human health and environmental factors, and cover all stages of the life cycle of a product containing nanomaterials.

The evaluation of the tools suggested that many of them are designed to screen risks, and not necessarily to support regulatory risk assessment. However, their strengths include a growing trend in quantitative models, which can assess uncertainty; for example, one tool analysed can identify uncertainties in its results that are due to gaps in knowledge about a material’s origin, characteristics and use.

The researchers also identified a growing trend in tools that provide protocols for experiments, such as identifying materials and test hazards, which are reproducible across laboratories. These tools could lead to a shift from expensive case-by-case testing for risk assessment of manufactured nanomaterials towards a more efficient process based on groupings of nanomaterials; and ‘read-across’ methods, where the properties of one material can be inferred without testing, based on the known properties of a similar material. The researchers do note, however, that although read-across methods are well established for chemical substances, they are still being developed for nanomaterials. To improve nanomaterial read-across methods, they suggest that more data are needed on the links between nanomaterials’ specific properties and their biological effects.

That’s all, folks.

Plasmonic ‘Goldfinger’: antifungal nail polish with metallic nanoparticles

A March 29,.2017 news item on Nanowerk announces a new kind of nanopolish,

Since ancient times, people have used lustrous silver, platinum and gold to make jewelry and other adornments. Researchers have now developed a new way to add the metals to nail polish with minimal additives, resulting in durable, tinted — and potentially antibacterial — nail coloring.

Using metal nanoparticles in clear nail polish makes it durable and colorful without extra additives.
Credit: American Chemical Society

A March 29, 2017 American Chemical Society (ACS) news release (also on EurekAlert), which originated the news item, adds a little more detail (Note: A link has been removed),

Nail polish comes in a bewildering array of colors. Current coloring techniques commonly incorporate pigment powders and additives. Scientists have recently started exploring the use of nanoparticles in polishes and have found that they can improve their durability and, in the case of silver nanoparticles, can treat fungal toenail infections. Marcus Lau, Friedrich Waag and Stephan Barcikowski wanted to see if they could come up with a simple way to integrate metal nanoparticles in nail polish.

The researchers started with store-bought bottles of clear, colorless nail polish and added small pieces of silver, gold, platinum or an alloy to them. To break the metals into nanoparticles, they shone a laser on them in short bursts over 15 minutes. Analysis showed that the method resulted in a variety of colored, transparent polishes with a metallic sheen. The researchers also used laser ablation to produce a master batch of metal nanoparticles in ethyl acetate, a polish thinner, which could then be added to individual bottles of polish. This could help boost the amount of production for commercialization. The researchers say the technique could also be used to create coatings for medical devices.

The authors acknowledge funding from the INTERREG-Program Germany-Netherlands.

A transparent nail varnish can be colored simply and directly with laser-generated nanoparticles. This does not only enable coloring of the varnish for cosmetic purposes, but also gives direct access to nanodoped varnishes to be used on any solid surface. Therefore, nanoparticle properties such as plasmonic properties or antibacterial effects can be easily adapted to surfaces for medical or optical purposes. The presented method for integration of metal (gold, platinum, silver, and alloy) nanoparticles into varnishes is straightforward and gives access to nanodoped polishes with optical properties, difficult to be achieved by dispersing powder pigments in the high-viscosity liquids. Courtesy: Industrial and Engineering & Chemistry Research

Here’s a link to and a citation for the paper,

Direct Integration of Laser-Generated Nanoparticles into Transparent Nail Polish: The Plasmonic “Goldfinger” by Marcus Lau, Friedrich Waag, and Stephan Barcikowski. Ind. Eng. Chem. Res., 2017, 56 (12), pp 3291–3296 DOI: 10.1021/acs.iecr.7b00039 Publication Date (Web): March 7, 2017

Copyright © 2017 American Chemical Society

This paper is behind a paywall.

Nanozymes as an antidote for pesticides

Should you have concerns about exposure to pesticides or chemical warfare agents (timely given events in Syria as per this April 4, 2017 news item on CBC [Canadian Broadcasting News Corporation] online) , scientists at the Lomonosov Moscow State University have developed a possible antidote according to a March 8,, 2017 news item on phys.org,

Members of the Faculty of Chemistry of the Lomonosov Moscow State University have developed novel nanosized agents that could be used as efficient protective and antidote modalities against the impact of neurotoxic organophosphorus compounds such as pesticides and chemical warfare agents. …

A March 7, 2017 Lomonosov Moscow State University press release on EurekAlert, which originated the news item, describes the work in detail,

A group of scientists from the Faculty of Chemistry under the leadership of Prof. Alexander Kabanov has focused their research supported by a “megagrant” on the nanoparticle-based delivery to an organism of enzymes, capable of destroying toxic organophosphorous compounds. Development of first nanosized drugs has started more than 30 years ago and already in the 90-s first nanomedicines for cancer treatment entered the market. First such medicines were based on liposomes – spherical vesicles made of lipid bilayers. The new technology, developed by Kabanov and his colleagues, uses an enzyme, synthesized at the Lomonosov Moscow State University, encapsulated into a biodegradable polymer coat, based on an amino acid (glutamic acid).

Alexander Kabanov, Doctor of Chemistry, Professor at the Eshelman School of Pharmacy of the University of North Carolina (USA) and the Faculty of Chemistry, M. V. Lomonosov Moscow State University, one of the authors of the article explains: “At the end of the 80-s my team (at that time in Moscow) and independently Japanese colleagues led by Prof. Kazunori Kataoka from Tokyo began using polymer micelles for small molecules delivery. Soon the nanomedicine field has “exploded”. Currently hundreds of laboratories across the globe work in this area, applying a wide variety of approaches to creation of such nanosized agents. A medicine on the basis of polymeric micelles, developed by a Korean company Samyang Biopharm, was approved for human use in 2006.”

Professor Kabanov’s team after moving to the USA in 1994 focused on development of polymer micelles, which could include biopolymers due to electrostatic interactions. Initially chemists were interested in usage of micelles for RNA and DNA delivery but later on scientists started actively utilizing this approach for delivery of proteins and, namely, enzymes, to the brain and other organs.

Alexander Kabanov says: “At the time I worked at the University of Nebraska Medical Center, in Omaha (USA) and by 2010 we had a lot of results in this area. That’s why when my colleague from the Chemical Enzymology Department of the Lomonosov Moscow State University, Prof. Natalia Klyachko offered me to apply for a megagrant the research theme of the new laboratory was quite obvious. Specifically, to use our delivery approach, which we’ve called a “nanozyme”, for “improvement” of enzymes, developed by colleagues at the Lomonosov Moscow State University for its further medical application.”

Scientists together with the group of enzymologists from the Lomonosov Moscow State University under the leadership of Elena Efremenko, Doctor of Biological Sciences, have chosen organophosphorus hydrolase as a one of the delivered enzymes. Organophosphorus hydrolase is capable of degrading toxic pesticides and chemical warfare agents with very high rate. However, it has disadvantages: because of its bacterial origin, an immune response is observed as a result of its delivery to an organism of mammals. Moreover, organophosphorus hydrolase is quickly removed from the body. Chemists have solved this problem with the help of a “self-assembly” approach: as a result of inclusion of organophosphorus hydrolase enzyme in a nanozyme particles the immune response becomes weaker and, on the contrary, both the storage stability of the enzyme and its lifetime after delivery to an organism considerably increase. Rat experiments have proved that such nanozyme efficiently protects organisms against lethal doses of highly toxic pesticides and even chemical warfare agents, such as VX nerve gas.

Alexander Kabanov summarizes: “The simplicity of our approach is very important. You could get an organophosphorus hydrolase nanozyme by simple mixing of aqueous solutions of anenzyme and safe biocompatible polymer. This nanozyme is self-assembled due to electrostatic interaction between a protein (enzyme) and polymer”.

According to the scientist’s words the simplicity and technological effectiveness of the approach along with the obtained promising results of animal experiments bring hope that this modality could be successful and in clinical use.

Members of the Faculty of Chemistry of the Lomonosov Moscow State University, along with scientists from the 27th Central Research Institute of the Ministry of Defense of the Russian Federation, the Eshelman School of Pharmacy of the University of North Carolina at Chapel Hill (USA) and the University of Nebraska Medical Center (UNC) have taken part in the Project.

Here’s a link to and a citation for the paper,

A simple and highly effective catalytic nanozyme scavenger for organophosphorus neurotoxins by Elena N. Efremenko, Ilya V. Lyagin, Natalia L. Klyachko, Tatiana Bronich, Natalia V. Zavyalova, Yuhang Jiang, Alexander V. Kabanov. Journal of Controlled Release Volume 247, 10 February 2017, Pages 175–181  http://dx.doi.org/10.1016/j.jconrel.2016.12.037

This paper is behind a paywall.

The Swiss come to a better understanding of nanomaterials

Just to keep things interesting, after the report suggesting most of the information that the OECD (Organization for Economic Cooperation and Development) has on nanomaterials is of little value for determining risk (see my April 5, 2017 posting for more) the Swiss government has released a report where they claim an improved understanding of nanomaterials than they previously had due to further research into the matter. From an April 6, 2017 news item on Nanowerk,

In the past six years, the [Swiss] National Research Programme “Opportunities and Risks of Nanomaterials” (NRP 64) intensively studied the development, use, behaviour and degradation of engineered nanomaterials, including their impact on humans and on the environment.

Twenty-three research projects on biomedicine, the environment, energy, construction materials and food demonstrated the enormous potential of engineered nanoparticles for numerous applications in industry and medicine. Thanks to these projects we now know a great deal more about the risks associated with nanomaterials and are therefore able to more accurately determine where and how they can be safely used.

An April 6, 2017 Swiss National Science Foundation press release, which originated the news item, expands on the theme,

“One of the specified criteria in the programme was that every project had to examine both the opportunities and the risks, and in some cases this was a major challenge for the researchers,” explains Peter Gehr, President of the NRP 64 Steering Committee.

One development that is nearing industrial application concerns a building material strengthened with nanocellulose that can be used to produce a strong but lightweight insulation material. Successful research was also carried out in the area of energy, where the aim was to find a way to make lithium-ion batteries safer and more efficient.

Promising outlook for nanomedicine

A great deal of potential is predicted for the field of nanomedicine. Nine of the 23 projects in NRP 64 focused on biomedical applications of nanoparticles. These include their use for drug delivery, for example in the fight against viruses, or as immune modulators in a vaccine against asthma. Another promising application concerns the use of nanomagnets for filtering out harmful metallic substances from the blood. One of the projects demonstrated that certain nanoparticles can penetrate the placenta barrier, which points to potential new therapy options. The potential of cartilage and bone substitute materials based on nanocellulose or nanofibres was also studied.

The examination of potential health risks was the focus of NRP 64. A number of projects examined what happens when nanoparticles are inhaled, while two focused on ingestion. One of these investigated whether the human gut is able to absorb iron more efficiently if it is administered in the form of iron nanoparticles in a food additive, while the other studied silicon nanoparticles as they occur in powdered condiments. It was ascertained that further studies will be required in order to determine the doses that can be used without risking an inflammatory reaction in the gut.

What happens to engineered nanomaterials in the environment?

The aim of the seven projects focusing on environmental impact was to gain a better understanding of the toxicity of nanomaterials and their degradability, stability and accumulation in the environment and in biological systems. Here, the research teams monitored how engineered nanoparticles disseminate along their lifecycle, and where they end up or how they can be discarded.

One of the projects established that 95 per cent of silver nanoparticles that are washed out of textiles are collected in sewage treatment plants, while the remaining particles end up in sewage sludge, which in Switzerland is incinerated. In another project a measurement device was developed to determine how aquatic microorganisms react when they come into contact with nanoparticles.

Applying results and making them available to industry

“The findings of the NRP 64 projects form the basis for a safe application of nanomaterials,” says Christoph Studer from the Federal Office of Public Health. “It has become apparent that regulatory instruments such as testing guidelines will have to be adapted at both national and international level.” Studer has been closely monitoring the research programme in his capacity as the Swiss government’s representative in NRP 64. In this context, the precautionary matrix developed by the government is an important instrument by means of which companies can systematically assess the risks associated with the use of nanomaterials in their production processes.

The importance of standardised characterisation and evaluation of engineered nanomaterials was highlighted by the close cooperation among researchers in the programme. “The research network that was built up in the framework of NRP 64 is functioning smoothly and needs to be further nurtured,” says Professor Bernd Nowack from Empa, who headed one of the 23 projects.

The results of NRP 64 show that new key technologies such as the use of nanomaterials need to be closely monitored through basic research due to the lack of data on its long-term effects. As Peter Gehr points out, “We now know a lot more about the risks of nanomaterials and how to keep them under control. However, we need to conduct additional research to learn what happens when humans and the environment are exposed to engineered nanoparticles over longer periods, or what happens a long time after a one-off exposure.”

You can find out more about the Opportunities and Risks of Nanomaterials; National Research Programme (NRP 64) here.

OECD (Organization for Economic Cooperation and Development) Dossiers on Nanomaterials Are of “Little to No Value for assessing risk?”

The announcement that a significant portion of the OECD’s (Organization for Economic Cooperation and Development) dossiers on 11 nanomaterials have next to no value for assessing risk seems a harsh judgment from the Center for International Environmental Law (CIEL). From a March 1, 2017 posting by Lynn L. Bergeson on the Nanotechnology Now,

On February 23, 2017, the Center for International Environmental Law (CIEL) issued a press release announcing a new report, commissioned by CIEL, the European Environmental Citizens’ Organization for Standardization (ECOS), and the Oeko-Institute, that “shows that most of the information made available by the Sponsorship Testing Programme of the Organisation for Economic Co-operation and Development (OECD) is of little to no value for the regulatory risk assessment of nanomaterials.”

Here’s more from the Feb. 23, 3017 CIEL press release, which originated the posting,

The study published today [Feb. 23, 2017] was delivered by the Institute of Occupational Medicine (IOM) based in Singapore. IOM screened the 11,500 pages of raw data of the OECD dossiers on 11 nanomaterials, and analysed all characterisation and toxicity data on three specific nanomaterials – fullerenes, single-walled carbon nanotubes, and zinc oxide.

“EU policy makers and industry are using the existence of the data to dispel concerns about the potential health and environmental risks of manufactured nanomaterials,” said David Azoulay, Senior Attorney for CIEL. “When you analyse the data, in most cases, it is impossible to assess what material was actually tested. The fact that data exists about a nanomaterial does not mean that the information is reliable to assess the hazards or risks of the material.”

The dossiers were published in 2015 by the OECD’s Working Party on Manufactured Nanomaterials (WPMN), which has yet to draw conclusions on the data quality. Despite this missing analysis, some stakeholders participating in EU policy-making – notably the European Chemicals Agency (ECHA) and the European Commission’s Joint Research Centre – have presented the dossiers as containing information on nano-specific human health and environmental impacts. Industry federations and individual companies have taken this a step further emphasizing that there is enough information available to discard most concerns about potential health or environmental risks of manufactured nanomaterials.

“Our study shows these claims that there is sufficient data available on nanomaterials are not only false, but dangerously so,” said Doreen Fedrigo, Senior Policy Officer of ECOS. ”The lack of nano-specific information in the dossiers means that the results of the tests cannot be used as evidence of no ‘nano-effect’ of the tested material. This information is crucial for regulators and producers who need to know the hazard profile of these materials. Analysing the dossiers has shown that legislation detailing nano-specific information requirements is crucial for the regulatory risk assessment of nanomaterials.”

The report provides important recommendations on future steps in the governance of nanomaterials. “Based on our analysis, serious gaps in current dossiers must be filled in with characterisation information, preparation protocols, and exposure data,” said Andreas Hermann of the Oeko-Institute. “Using these dossiers as they are and ignoring these recommendations would mean making decisions on the safety of nanomaterials based on faulty and incomplete data. Our health and environment requires more from producers and regulators.”

CIEL has an Analysis of OECD WPMN Dossiers Regarding the Availability of Data to Evaluate and Regulate Risk (Dec 2016) webpage which provides more information about the dossiers and about the research into the dossiers and includes links to the report, the executive summer, and the dataset,

The Sponsorship Testing Programme of the Working Party on Manufactured Nanomaterials (WPMN) of the Organisation for Economic Co-operation and Development (OECD) started in 2007 with the aim to test a selection of 13 representative nanomaterials for many endpoints. The main objectives of the programme were to better understand what information on intrinsic properties of the nanomaterials might be relevant for exposure and hazards assessment and assess the validity of OECD chemicals Test Guidelines for nanomaterials. The testing programme concluded in 2015 with the publication of dossiers on 11 nanomaterials: 11,500 pages of raw data to be analysed and interpreted.

The WPMN has not drawn conclusions on the data quality, but some stakeholders participating in EU policy-making – notably the European Chemicals Agency and the European Commission’s Joint Research Centre – presented the dossiers as containing much scientific information that provided a better understanding of their nano-specific human health and environmental impacts. Industry federations and individual companies echoed the views, highlighting that there was enough information available to discard most concerns about potential health or environmental risks of manufactured nanomaterials.

As for the OECD, it concluded, even before the publication of the dossiers, that “many of the existing guidelines are also suitable for the safety assessment of nanomaterials” and “the outcomes (of the sponsorship programme) will provide useful information on the ‘intrinsic properties’ of nanomaterials.”

The Center for International Environmental Law (CIEL), the European Citizens’ Organisation for Standardisation (ECOS) and the Öko-Institut commissioned scientific analysis of these dossiers to assess the relevance of the data for regulatory risk assessment.

The resulting report: Analysis of OECD WPMN dossiers regarding the availability of data to evaluate and regulate risk, provides insights illustratating how most of the information made available by the sponsorship programme is of little to no value in identifying hazards or in assessing risks due to nanomaterials.

The analysis shows that:

  • Most studies and documents in the dossiers contain insufficient characterisation data about the specific nanomaterial addressed (size, particle distribution, surface shape, etc.), making it impossible to assess what material was actually tested.
  • This makes it impossible to make any firm statements regarding the nano-specificity of the hazard data published, or the relationship between observed effects and specific nano-scale properties.
  • Less than 2% of the study records provide detail on the size of the nanomaterial tested. Most studies use mass rather than number or size distribution (so not following scientifically recommended reporting practice).
  • The absence of details on the method used to prepare the nanomaterial makes it virtually impossible to correlate an identified hazard with specific nanomaterial characteristic. Since the studies do not indicate dispersion protocols used, it is impossible to assess whether the final dispersion contained the intended mass concentration (or even the actual presence of nanomaterials in the test system), how much agglomeration may have occurred, and how the preparation protocols may have influenced the size distribution.
  • There is not enough nano-specific information in the dossiers to inform about nano-characteristics of the raw material that influence their toxicology. This information is important for regulators and its absence makes information in the dossier irrelevant to develop read-across guidelines.
  • Only about half of the endpoint study records using OECD Test Guideliness (TGs) were delivered using unaltered OECD TGs, thereby respecting the Guidelines’ requirements. The reasons for modifications of the TGs used in the tests are not clear from the documentation. This includes whether the study record was modified to account for challenges related to specific nanomaterial properties or for other, non-nano-specific reasons.
  • The studies do not contain systematic testing of the influence of nano-specific characteristics on the study outcome, and they do not provide the data needed to assess the effect of nano-scale features on the Test Guidelines. Given the absence of fundamental information on nanomaterial characteristics, the dossiers do not provide evidence of the applicability of existing OECD Test Guidelines to nanomaterials.

The analysis therefore dispels several myths created by some stakeholders following publication of the dossiers and provides important perspective for the governance of nanomaterials. In particular, the analysis makes recommendations to:

  • Systematically assess the validity of existing Test Guidelines for relevance to nanomaterials
  • Develop Test Guidelines for dispersion and other test preparations
  • Define the minimum characteristics of nanomaterials that need to be reported
  • Support the build-up of exposure database
  • Fill the gaps in current dossiers with characterisation information, preparation protocols and exposure data

Read full report.
Read executive summary.
Download full dataset.

This is not my area of expertise and while I find the language a bit inflammatory, it’s my understanding that there are great gaps in our understanding of nanomaterials and testing for risk assessment has been criticized for many of the reasons pointed out by CIEL, ECOS, and the Oeko-Institute.

You can find out more about CIEL here; ECOS here; and the Oeko-Institute (also known as Öko-Institute) here.

Singing posters and talking shirts can communicate with you via car radio or smartphones

Singing posters and talking shirts haven’t gone beyond the prototype stage yet but I imagine University of Washington engineers are hoping this will happen sooner rather than later. In the meantime, they are  presenting their work at a conference according to a March 1, 2017 news item on ScienceDaily,

Imagine you’re waiting in your car and a poster for a concert from a local band catches your eye. What if you could just tune your car to a radio station and actually listen to that band’s music? Or perhaps you see the poster on the side of a bus stop. What if it could send your smartphone a link for discounted tickets or give you directions to the venue?

Going further, imagine you go for a run, and your shirt can sense your perspiration and send data on your vital signs directly to your phone.

A new technique pioneered by University of Washington engineers makes these “smart” posters and clothing a reality by allowing them to communicate directly with your car’s radio or your smartphone. For instance, bus stop billboards could send digital content about local attractions. A street sign could broadcast the name of an intersection or notice that it is safe to cross a street, improving accessibility for the disabled. In addition, clothing with integrated sensors could monitor vital signs and send them to a phone. [emphasis mine]

“What we want to do is enable smart cities and fabrics where everyday objects in outdoor environments — whether it’s posters or street signs or even the shirt you’re wearing — can ‘talk’ to you by sending information to your phone or car,” said lead faculty and UW assistant professor of computer science and engineering Shyam Gollakota.

“The challenge is that radio technologies like WiFi, Bluetooth and conventional FM radios would last less than half a day with a coin cell battery when transmitting,” said co-author and UW electrical engineering doctoral student Vikram Iyer. “So we developed a new way of communication where we send information by reflecting ambient FM radio signals that are already in the air, which consumes close to zero power.”

The UW team has — for the first time — demonstrated how to apply a technique called “backscattering” to outdoor FM radio signals. The new system transmits messages by reflecting and encoding audio and data in these signals that are ubiquitous in urban environments, without affecting the original radio transmissions. Results are published in a paper to be presented in Boston at the 14th USENIX Symposium on Networked Systems Design and Implementation in March [2017].

The team demonstrated that a “singing poster” for the band Simply Three placed at a bus stop could transmit a snippet of the band’s music, as well as an advertisement for the band, to a smartphone at a distance of 12 feet or to a car over 60 feet away. They overlaid the audio and data on top of ambient news signals from a local NPR radio station.

The University of Washington has produced a video demonstration of the technology

A March 1, 2017 University of Washington news release (also on EurekAlert), which originated the news item, explains further (Note: Links have been removed),

“FM radio signals are everywhere. You can listen to music or news in your car and it’s a common way for us to get our information,” said co-author and UW computer science and engineering doctoral student Anran Wang. “So what we do is basically make each of these everyday objects into a mini FM radio station at almost zero power.”

Such ubiquitous low-power connectivity can also enable smart fabric applications such as clothing integrated with sensors to monitor a runner’s gait and vital signs that transmits the information directly to a user’s phone. In a second demonstration, the researchers from the UW Networks & Mobile Systems Lab used conductive thread to sew an antenna into a cotton T-shirt, which was able to use ambient radio signals to transmit data to a smartphone at rates up to 3.2 kilobits per second.

The system works by taking an everyday FM radio signal broadcast from an urban radio tower. The “smart” poster or T-shirt uses a low-power reflector to manipulate the signal in a way that encodes the desired audio or data on top of the FM broadcast to send a “message” to the smartphone receiver on an unoccupied frequency in the FM radio band.

“Our system doesn’t disturb existing FM radio frequencies,” said co-author Joshua Smith, UW associate professor of computer science and engineering and of electrical engineering. “We send our messages on an adjacent band that no one is using — so we can piggyback on your favorite news or music channel without disturbing the original transmission.”

The team demonstrated three different methods for sending audio signals and data using FM backscatter: one simply overlays the new information on top of the existing signals, another takes advantage of unused portions of a stereo FM broadcast, and the third uses cooperation between two smartphones to decode the message.

“Because of the unique structure of FM radio signals, multiplying the original signal with the backscattered signal actually produces an additive frequency change,” said co-author Vamsi Talla, a UW postdoctoral researcher in computer science and engineering. “These frequency changes can be decoded as audio on the normal FM receivers built into cars and smartphones.”

In the team’s demonstrations, the total power consumption of the backscatter system was 11 microwatts, which could be easily supplied by a tiny coin-cell battery for a couple of years, or powered using tiny solar cells.

I cannot help but notice the interest in using this technology is for monitoring purposes, which could be benign or otherwise.

For anyone curious about the 14th USENIX Symposium on Networked Systems Design and Implementation being held March 27 – 29, 2017 in Boston, Massachusetts, you can find out more here.

Findings on oral exposure to nanoscale titanium dioxide

It’s been a while since I’ve run a piece on health concerns and nanoparticles. The nanoparticles in question are titanium dioxide and the concerns centre on oral exposure to them according to a Jan. 24, 2017 news item on Nanowerk,

Researchers from INRA [French National Institute for Agricultural Research] and their partners have studied the effects of oral exposure to titanium dioxide, an additive (E171) commonly used in foodstuffs, especially confectionary. They have shown for the first time that E171 crosses the intestinal barrier in animals and reaches other parts of the body.

Immune system disorders linked to the absorption of the nanoscale fraction of E171 particles were observed. The researchers also showed that chronic oral exposure to the additive spontaneously induced preneoplastic lesions in the colon, a non-malignant stage of carcinogenesis, in 40% of exposed animals.

Moreover, E171 was found to accelerate the development of lesions previously induced for experimental purposes. While the findings show that the additive plays a role in initiating and promoting the early stages of colorectal carcinogenesis, they cannot be extrapolated to humans or more advanced stages of the disease. [emphasis mine]

A Jan. 20, 2017 IINRA press release, which originated the news item,  provides more detail about European use of titanium dioxide as a food additive and about the research,

Present in many products including cosmetics, sunscreens, paint and building materials, titanium dioxide (or TiO2), known as E171 in Europe, is also widely used as an additive in the food industry to whiten or give opacity to products. It is commonly found in sweets, chocolate products, biscuits, chewing gum and food supplements, as well as in toothpaste and pharmaceutical products. Composed of micro- and nanoparticles, E171 is nevertheless not labelled a “nanomaterial”, since it does not contain more than 50% of nanoparticles (in general it contains from 10-40%). The International Agency for Research on Cancer (IARC) evaluated the risk of exposure to titanium dioxide by inhalation (occupational exposure), resulting in a Group 2B classification, reserved for potential carcinogens for humans.

Today, oral exposure to E171 is a concern, especially in children who tend to eat a lot of sweets. INRA researchers studied the product as a whole (that is, its mixed composition of micro- and nanoparticules), and have also evaluated the effect of the nanoscale particle fraction alone, by comparing it to a model nanoparticle.

Titanium dioxide crosses the intestinal barrier and passes into the bloodstream

The researchers exposed rats orally to a dose of 10mg of E171 per kilogram of body weight per day, similar to the exposure humans experience through food consumption (data from European Food Safety Agency, September 20162). They showed for the first time in vivo that titanium dioxide is absorbed by the intestine and passes into the bloodstream. Indeed, the researchers found titanium dioxide particles in the animals’ livers.

Titanium dioxide alters intestinal and systemic immune response

Titanium dioxide nanoparticles were present in the lining of the small intestine and in the colon, and entered the nuclei of the immune cells of Peyer’s patches, which induce immune response in the intestine. The researchers showed an imbalance in immune response, ranging from a defect in the production of cytokines in Peyer’s patches to the development of micro-inflammation in colon mucosa. In the spleen, representative of systemic immunity, exposure to E171 increases the capacity of immune cells to produce pro-inflammatory cytokines when they are activated in vitro.

Chronic oral exposure to titanium dioxide plays a role in initiating and promoting early stages of colorectal carcinogenesis

The researchers exposed rats to regular oral doses of titanium dioxide through drinking water for 100 days. In a group of rats previously treated with an experimental carcinogen, exposure to TiO2 led to an increase in the size of preneoplastic lesions. In a group of healthy rats exposed to E171, four out of eleven spontaneously developed preneoplastic lesions in the intestinal epithelium. Non-exposed animals presented no anomalies at the end of the 100-day study. These results indicate that E171 both initiates and promotes the early stages of colorectal carcinogenesis in animals.

These studies show for the first time that the additive E171 is a source of titanium dioxide nanoparticles in the intestine and the entire body, with consequences for both immune function and the development of preneoplastic lesions in the colon. These first findings justify a carcinogenesis study carried out under OECD [Organization for Economic Cooperation and Development] guidelines to continue observations at a later stage of cancer. They provide new data for evaluating the risks of the E171 additive in humans.

These studies were carried out within the framework of the Nanogut project, financed by the French Agency for Food, Environmental and Occupational Health & Safety (ANSES) within the French national programme for research related to the environment, health and the workplace (PNR EST) and coordinated by INRA. Sarah Bettini’s university thesis contract was financed by the French laboratory of excellence LabEx SERENADE.

Here’s a link to and a citation for the paper,

Food-grade TiO2 impairs intestinal and systemic immune homeostasis, initiates preneoplastic lesions and promotes aberrant crypt development in the rat colon by Sarah Bettini, Elisa Boutet-Robinet, Christel Cartier, Christine Coméra, Eric Gaultier, Jacques Dupuy, Nathalie Naud, Sylviane Taché, Patrick Grysan, Solenn Reguer, Nathalie Thieriet, Matthieu Réfrégiers, Dominique Thiaudière, Jean-Pierre Cravedi, Marie Carrière, Jean-Nicolas Audinot, Fabrice H. Pierre, Laurence Guzylack-Piriou, & Eric Houdeau. Scientific Reports 7, Article number: 40373 (2017) doi:10.1038/srep40373 Published online: 20 January 2017

This paper is open access.

The research is concerning but they don’t want to draw any conclusions yet, which explains the recommendation for further research.

Using sugar for a better way to clean nanoparticles from organisms

Researchers at the US National Institute of Standards and Technology (NIST) have found that a laboratory technique used for over 60 years is the best way to date to clean nanoparticles from organisms. From a Jan. 26, 2017 news item on ScienceDaily,

Sometimes old-school methods provide the best ways of studying cutting-edge tech and its effects on the modern world.

Giving a 65-year-old laboratory technique a new role, researchers at the National Institute of Standards and Technology (NIST) have performed the cleanest separation to date of synthetic nanoparticles from a living organism. The new NIST method is expected to significantly improve experiments looking at the potential environmental and health impacts of these manufactured entities. It will allow scientists to more accurately count how many nanoparticles have actually been ingested by organisms exposed to them.

A Jan. 26, 2017 NIST news release (also on EurekAlert), which originated the news item, offers more detail,

The common roundworm Caenorhabditis elegans has been used in recent years as a living model for laboratory studies of how biological and chemical compounds may affect multicellular organisms. These compounds include engineered nanoparticles (ENPs), bits of material between 1 and 100 nanometers (billionths of a meter, or about 1/10,000 the diameter of a red blood cell). Previous research has often focused on quantifying the amount and size of engineered nanoparticles ingested by C. elegans. Measuring the nanoparticles that actually make it into an organism is considered a more relevant indicator of potential toxicity than just the amount of ENPs to which the worms are exposed.

Traditional methods for counting ingested ENPs have produced questionable results. Currently, researchers expose C. elegans to metal ENPs such as silver or gold in solution, then rinse the excess particles away with water followed by centrifugation and freeze-drying. A portion of the “cleaned” sample produced is then typically examined by a technique that determines the amount of metal present, known as inductively coupled plasma mass spectrometry (ICP-MS). It often yields ENP counts in the tens of thousands per worm; however, those numbers always seem too high to NIST researchers working with C. elegans.

“Since ICP-MS will detect all of the nanoparticles associated with the worms, both those ingested and those that remain attached externally, we suspect that the latter is what makes the ‘ENPs’ per-worm counts so high,” said NIST analytical chemist Monique Johnson (link sends e-mail), the lead author on the ACS Nano paper. “Since we only wanted to quantify the ingested ENPs, a more robust and reliable separation method was needed.”

Luckily, the solution to the problem was already in the lab.

Cross section of the roundworm C. elegans

Scanning electron micrograph showing a cross section of the roundworm C. elegans with two ingested engineered nanoparticles (red dots just right of center). Images such as this provided NIST researchers with visual confirmation that nanoparticle consumption actually occurred. Credit: K. Scott/NIST

In the course of culturing C. elegans for ENP-exposure experiments, Johnson and her colleagues had used sucrose density gradient centrifugation, a decades-old and established system for cleanly separating cellular components, to isolate the worms from debris and bacteria. “We wondered if the same process would allow us to perform an organism-from-ENP separation as well, so I designed a study to find out,” Johnson said.

In their experiment, the NIST researchers first exposed separate samples of C. elegans to low and high concentrations of two sizes of gold nanospheres, 30 and 60 nanometers in diameter. The researchers put each of the samples into a centrifuge and removed the supernatant (liquid portion), leaving the worms and ENPs in the remaining pellets. These were centrifuged twice in a salt solution (rather than just water as in previous separation methods), and then centrifuged again, but this time, through a uniquely designed sucrose density gradient.

“From top to bottom, our gradient consisted of a salt solution layer to trap excess ENPs and three increasingly dense layers of sucrose [20, 40 and 50 percent] to isolate the C. elegans,” Johnson explained. “We followed up the gradient with three water rinses and with centrifugations to ensure that only worms with ingested ENPs, and not the sucrose separation medium with any excess ENPs, would make it into the final pellet.”

Analyzing the range of masses in the ultrapurified samples indicated gold levels more in line with what the researchers expected would be found as ingested ENPs. Experimental validation of the NIST separation method’s success came when the worms were examined in detail under a scanning electron microscope (SEM).

“For me, the eureka moment was when I first saw gold ENPs in the cross section images taken from the C. elegans samples that had been processed through the sucrose density gradient,” Johnson said. “I had been dreaming about finding ENPs in the worm’s digestive tract and now they were really there!”

The high-resolution SEM images also provided visual evidence that only ingested ENPs were counted. “No ENPs were attached to the cuticle, the exoskeleton of C. elegans, in any of the sucrose density gradient samples,” Johnson said. “When we examined worms from our control experiments [processed using the traditional no-gradient, water-rinse-only separation method], there were a number of nanospheres found attached to the cuticle.

Now that it has been successfully demonstrated, the NIST researchers plan to refine and further validate their system for evaluating the uptake of ENPs by C. elegans. “Hopefully, our method will become a useful and valuable tool for reducing the measurement variability and sampling bias that can plague environmental nanotoxicology studies,” Johnson said.

They’ve tested this technique on gold nanoparticles, which begs the question, What kinds of nanoparticles can this technique be used for? Metal nanoparticles only or all nanoparticles?

I’m sure the researchers have already asked these questions and started researching the answers. While the rest of us wait, here’s a link to and a citation for the paper about this promising new technique,

Separation, Sizing, and Quantitation of Engineered Nanoparticles in an Organism Model Using Inductively Coupled Plasma Mass Spectrometry and Image Analysis by Monique E. Johnson, Shannon K. Hanna, Antonio R. Montoro Bustos, Christopher M. Sims, Lindsay C. C. Elliott, Akshay Lingayat, Adrian C. Johnston, Babak Nikoobakht, John T. Elliott, R. David Holbrook, Keana C. K. Scott, Karen E. Murphy, Elijah J. Petersen, Lee L. Yu, and Bryant C. Nelson. ACS Nano, 2017, 11 (1), pp 526–540 DOI: 10.1021/acsnano.6b06582 Publication Date (Web): December 16, 2016

Copyright This article not subject to U.S. Copyright. Published 2016 by the American Chemical Society

This paper is behind a paywall.

Nanoparticles can activate viruses lying dormant in lung cells

The nanoparticles in question are from combustion engines, which means that we are exposed to them. One other note, the testing has not been done on humans but rather on cells. From a Jan. 16, 2017 news item on ScienceDaily,

Nanoparticles from combustion engines can activate viruses that are dormant in in lung tissue cells. This is the result of a study by researchers of Helmholtz Zentrum München, a partner in the German Center for Lung Research (DZL), which has now been published in the journal Particle and Fibre Toxicology.

To evade the immune system, some viruses hide in cells of their host and persist there. In medical terminology, this state is referred to as a latent infection. If the immune system becomes weakened or if certain conditions change, the viruses become active again, begin to proliferate and destroy the host cell. A team of scientists led by Dr. Tobias Stöger of the Institute of Lung Biology and Prof. Dr. Heiko Adler, deputy head of the research unit Lung Repair and Regeneration at Helmholtz Zentrum München, now report that nanoparticles can also trigger this process.

A Jan. 16, 2017 Helmholtz Zentrum München press release (also on EurekAlert), which originated the news item, provides more detail,

“From previous model studies we already knew that the inhalation of nanoparticles has an inflammatory effect and alters the immune system,” said study leader Stöger. Together with his colleagues Heiko Adler and Prof. Dr. Philippe Schmitt-Kopplin, he showed that “an exposure to nanoparticles can reactivate latent herpes viruses in the lung.”

Specifically, the scientists tested the influence of nanoparticles typically generated by fossil fuel combustion in an experimental model for a particular herpes virus infection. They detected a significant increase in viral proteins, which are only produced with active virus proliferation. “Metabolic and gene expression analyses also revealed patterns resembling acute infection,” said Philippe Schmitt-Kopplin, head of the research unit Analytical BioGeoChemistry (BGC). Moreover, further experiments with human cells demonstrated that Epstein-Barr viruses are also ‘awakened’ when they come into contact with the nanoparticles.

Potential approach for chronic lung diseases

In further studies, the research team would like to test whether the results can also be transferred to humans. “Many people carry herpes viruses, and patients with idiopathic pulmonary fibrosis are particularly affected,” said Heiko Adler. “If the results are confirmed in humans, it would be important to investigate the molecular process of the reactivation of latent herpes viruses induced by particle inhalation. Then we could try to influence this pathway therapeutically.”

Special cell culture models shall therefore elucidate the exact mechanism of virus reactivation by nanoparticles. “In addition,” Stöger said, ”in long-term studies we would like to investigate to what extent  repeated nanoparticle exposure with corresponding virus reactivation leads to chronic inflammatory and remodeling processes in the lung.”

Further Information

Background:
In 2015 another group at the Helmholtz Zentrum München demonstrated how the Epstein-Barr virus  hides in human cells. In March 2016 researchers also showed that microRNAs silence immune alarm signals of cells infected with the Epstein-Barr virus.

Original Publication:
Sattler, C. et al. (2016): Nanoparticle exposure reactivates latent herpesvirus and restores a signature of acute infection. Particle and Fibre Toxicology, DOI 10.1186/s12989-016-0181-1

Here’s a link to and a citation for the paper on investigating latent herpes virus,

Nanoparticle exposure reactivates latent herpesvirus and restores a signature of acute infection by Christine Sattler, Franco Moritz, Shanze Chen, Beatrix Steer, David Kutschke, Martin Irmler, Johannes Beckers, Oliver Eickelberg, Philippe Schmitt-Kopplin, Heiko Adler. Particle and Fibre Toxicology201714:2 DOI: 10.1186/s12989-016-0181-1 Published: 10 January 2017

©  The Author(s). 2017

This paper is open access and, so too, is the 2016 paper.

XSEDE: the most advanced, powerful integrated digital resources in the world and nanomaterials

The University of Iowa does not jump to mind when considering powerhouse nanomaterial research; it seems that’s a mistake. An Oct. 19, 2016 news item on Nanowerk sets the record straight,

Chemists at the University of Iowa will research the effects of nanomaterials on the environment and human health using a network of supercomputers funded by the U.S. National Science Foundation.

Sara E. Mason, assistant professor in the Department of Chemistry, won an NSF award that grants her team access to the Extreme Science and Engineering Discovery Environment (XSEDE). The XSEDE project links computers, data, and people from around the world to establish a single, virtual system that scientists can interactively use to conduct research. It was started in 2011 and was renewed by the NSF last August.

The NSF says it “will be the most advanced, powerful, and robust collection of integrated advanced digital resources and services in the world.”

An Oct. 12, 2016 University of Iowa (UI) news release by Richard C. Lewis, which originated the news item, provides a little more detail,

The UI grant, valued at $72,503, essentially gives Mason’s team time on the supercomputer network, which they can access from their desktops. The researchers will use that time to study nanoparticles—matter far too small to be seen by the naked eye and present in a range of products, from sunscreen to advanced batteries for hybrid and electric vehicles.

The team hopes to better define the atom-to-atom interactions of various nanoparticles. Mason says the grant will “super charge” her computational research.

“To me, having four concurrent NSF research grants is a big deal, and now, having the boost of the computer time allows us to do even more,” Mason says. “XSEDE allows us to run simulations using quantum mechanics and highly parallelized computers. The outcome is new chemical insight into natural or widely used nanoparticles. We can then connect the chemistry to broader issues, such as human health and the behavior of nanomaterials in the environment.”

Mason’s group aims to find and design nanomaterials that are more benign to the environment and human health. Part of the search means trying out new elements in computational designs to find out how they interact, as well as their side effects, good or bad.

The XSEDE computers will give them far more computing horsepower to carry out those computational experiments.

“We can collectively get a lot more done in a shorter period of time,” says Joseph Bennett, co-principal investigator on the grant and a post-doctoral researcher in Mason’s group.

The UI is one of 15 institutions affiliated with the NSF-funded Center for Sustainable Nanotechnology, devoted to investigating the fundamental molecular mechanisms by which nanoparticles interact with biological systems.

I wish them good luck.