This July 22, 2016 news item (on Nanowerk) about bacteria and marine oil spill remediation was a little challenging (for me) to read (Note: A link has been removed),
A July 22, 2016 Kazan Federal University (Russia) press release (also on EurekAlert), which originated the news item, has more detail about the research,
Bionanotechnology research is targeted on functional structures synergistically combining macromolecules, cells, or multicellular assemblies with a wide range of nanomaterials. Providing micrometer-sized cells with tiny nanodevices expands the uses of the cultured microorganisms and requires nanoassembly on individual live cells (“Nanoshell Assembly for Magnet-Responsive Oil-Degrading Bacteria”).
Surface engineering functionalizes the cell walls with polymer layers and/or nanosized particles and has been widely employed to modify the intrinsic properties of microbial cells. Cell encapsulation allows fabricating live microbial cells with magnetic nanoparticles onto cell walls, which mimics natural magnetotactic bacteria.
For this study researchers from Kazan Federal University and Louisiana Tech University chose Alcanivorax borkumensis marine bacteria as a target microorganism for cell surface engineering with magnetic nanoparticles for the following reasons: (1) these hydrocarbon-degrading bacteria are regarded as an important tool in marine oil spill remediation and potentially can be used in industrial oil-processing bioreactors, therefore the external magnetic manipulations with these cells seems to be practically relevant; (2) A. borkumensis are marine Gram-negative species having relatively fragile and thin cell walls, which makes cell wall engineering of these bacteria particularly challenging.
Rendering oil-degrading bacteria with artificially added magnetic functionality is important to attenuate their properties and to expand their practical use.
Cell surface engineering was performed using polycation-coated magnetic nanoparticles, which is a fast and straightforward process utilizing the direct deposition of positively charged iron oxide nanoparticles onto microbial cells during a brief incubation in excessive concentrations of nanoparticles. Gram-negative bacteria cell walls are built from the thin peptidoglycan layer sandwiched between the outer membrane and inner plasma membrane, with lipopolysaccharides rendering the overall negative cell charge, therefore cationic particles will attach to the cell walls due to electrostatic interactions.
Rod-like 0.5-μm diameter Gram-negative bacteria A. borkumensis were coated with 70?100 nm [sic] magnetite shells. The deposition of nanoparticles was performed with extreme care to ensure the survival of magnetized cells.
The development of biofilms on hydrophobic surface is a very important feature of A. borkumensis cells because this is how these cells attach to the oil droplets in natural environments. Consequently, any cell surface modification should not reduce their ability to attach and proliferate as biofilms. Here, at all concentrations of PAH- magnetite nanoparticles investigated, authors of the study detected the similar biofilm growth patterns. Overall, the magnetized cells were able to proliferate and exhibited normal physiological activity.
The next generations of the bacteria have a tendency to remove the artificial shell returning to the native form. Such magnetic nanoencapsulation may be used for the A. borkumensis transportation in the bioreactors to enhance the spill oil decomposition at certain locations.
If I read this rightly, the idea, in future iterations of this research, is to destroy the oil once it’s been gathered by the biofilm. This seems a different approach where other oil spill remediation techniques have hydrophobic/oleophilic sponges absorbing the oil, which could potentially be used in the future. There are carbon nanotube sponges (my April 17, 2012 posting) and boron nitride sponges (my Dec. 7, 2015 posting).
Here’s a link to and a citation for the paper,
Nanoshell Assembly for Magnet-Responsive Oil-Degrading Bacteria by Svetlana A. Konnova, Yuri M. Lvov, and Rawil F. Fakhrullin. Langmuir, Article ASAP DOI: 10.1021/acs.langmuir.6b01743 Publication Date (Web): June 09, 2016
Copyright © 2016 American Chemical Society
This paper is behind a paywall.