Tag Archives: S. Grant

Making ordinary microscopes image objects at the nanoscale

The researchers believe this technique for making ordinary microscopes capable of nanoscale imaging will make research into diseases easier, especially in developing countries. A July 20, 2016 news item on phys.org announces the new technique,

Research completed through a collaboration with University of Missouri [MU] engineers, biologists, and chemists could transform how scientists study molecules and cells at sub-microscopic (nanoscale) levels. Shubra Gangopadhyay, an electrical and computer engineer and her team at MU recently published studies outlining a new, relatively inexpensive imaging platform that enables single molecule imaging. This patented method highlights Gangopadhyay’s more than 30 years of nanoscale research that has proven invaluable in biological research and battling diseases.

This diagram shows the difference between regular and plasmonic gratings in terms of fluorescent intensity. Credit: Shubhra Gangopadhyay/Nanoscale.

This diagram shows the difference between regular and plasmonic gratings in terms of fluorescent intensity. Credit: Shubhra Gangopadhyay/Nanoscale.

A July 19, 2016 University of Missouri news release (also received via email), which originated the news item, explains further,

“Usually, scientists have to use very expensive microscopes to image at the sub-microscopic level,” said Gangopadhyay, the C.W. LaPierre Endowed Chair of electrical and computer engineering in the MU College of Engineering. “The techniques we’ve established help to produce enhanced imaging results with ordinary microscopes. The relatively low production cost for the platform also means it could be used to detect a wide variety of diseases, particularly in developing countries.”

The team’s custom platform uses an interaction between light and the surface of the metal grating to generate surface plasmon resonance (SPR), a rapidly developing imaging technique that enables super-resolution imaging down to 65 nanometers—a resolution normally reserved for electron microscopes. Using HD-DVD and Blu-Ray discs as starting templates, a repeating grating pattern is transferred onto the microscope slides where the specimen will be placed. Since the patterns originate from a widely used technology, the manufacturing process remains relatively inexpensive.

“In previous studies, we’ve used plasmonic gratings to detect cortisol and even tuberculosis,” Gangopadhyay said. “Additionally, the relatively low production cost for the platform also means it could be used to further detect a wide variety of diseases, particularly in developing countries. Eventually, we might even be able to use smartphones to detect disease in the field.”

Here’s a link to and a citation for the paper,

Plasmonic gratings with nano-protrusions made by glancing angle deposition for single-molecule super-resolution imaging by B. Chen, A. Wood, A. Pathak, J. Mathai, S. Bok, H. Zheng, S. Hamm, S. Basuray, S. Grant, K. Gangopadhyay, P. V. Cornish, and S. Gangopadhyay. Nanoscale, 2016,8, 12189-12201 DOI: 10.1039/C5NR09165A First published online 24 May 2016

This paper is behind a paywall.

ETA July 22, 2016: Dexter Johnson’s July 21, 2016 posting provides both a neat summary and added detail from an engineer’s perspective.