Tag Archives: University of Rochester (NY)

Need to improve oversight on chimeric human-animal research

It seems chimeras are of more interest these days. In all likelihood that has something to do with the fellow who received a transplant of a pig’s heart in January 2022 (he died in March 2022).

For those who aren’t familiar with the term, a chimera is an entity with two different DNA (deoxyribonucleic acid) identities. In short, if you get a DNA sample from the heart, it’s different from a DNA sample obtained from a cheek swab. This contrasts with a hybrid such as a mule (donkey/horse) whose DNA samples show a consisted identity throughout its body.

A December 12, 2022 The Hastings Center news release (also on EurekAlert) announces a special report,

A new report on the ethics of crossing species boundaries by inserting human cells into nonhuman animals – research surrounded by debate – makes recommendations clarifying the ethical issues and calling for improved oversight of this work.

The report, “Creating Chimeric Animals — Seeking Clarity On Ethics and Oversight,” was developed by an interdisciplinary team, with funding from the National Institutes of Health. Principal investigators are Josephine Johnston and Karen Maschke, research scholars at The Hastings Center, and Insoo Hyun, director of the Center for Life Sciences and Public Learning at the Museum of Life Sciences in Boston, formerly of Case Western Reserve University.

Advances in human stem cell science and gene editing enable scientists to insert human cells more extensively and precisely into nonhuman animals, creating “chimeric” animals, embryos, and other organisms that contain a mix of human and nonhuman cells.

Many people hope that this research will yield enormous benefits, including better models of human disease, inexpensive sources of human eggs and embryos for research, and sources of tissues and organs suitable for transplantation into humans. 

But there are ethical concerns about this type of research, which raise questions such as whether the moral status of nonhuman animals is altered by the insertion of human stem cells, whether these studies should be subject to additional prohibitions or oversight, and whether this kind of research should be done at all.

The report found that:

Animal welfare is a primary ethical issue and should be a focus of ethical and policy analysis as well as the governance and oversight of chimeric research.

Chimeric studies raise the possibility of unique or novel harms resulting from the insertion and development of human stem cells in nonhuman animals, particularly when those cells develop in the brain or central nervous system.

Oversight and governance of chimeric research are siloed, and public communication is minimal. Public communication should be improved, communication between the different committees involved in oversight at each institution should be enhanced, and a national mechanism created for those involved in oversight of these studies. 

Scientists, journalists, bioethicists, and others writing about chimeric research should use precise and accessible language that clarifies rather than obscures the ethical issues at stake. The terms “chimera,” which in Greek mythology refers to a fire-breathing monster, and “humanization” are examples of ethically laden, or overly broad language to be avoided.

The Research Team

The Hastings Center

• Josephine Johnston
• Karen J. Maschke
• Carolyn P. Neuhaus
• Margaret M. Matthews
• Isabel Bolo

Case Western Reserve University
• Insoo Hyun (now at Museum of Science, Boston)
• Patricia Marshall
• Kaitlynn P. Craig

The Work Group

• Kara Drolet, Oregon Health & Science University
• Henry T. Greely, Stanford University
• Lori R. Hill, MD Anderson Cancer Center
• Amy Hinterberger, King’s College London
• Elisa A. Hurley, Public Responsibility in Medicine and Research
• Robert Kesterson, University of Alabama at Birmingham
• Jonathan Kimmelman, McGill University
• Nancy M. P. King, Wake Forest University School of Medicine
• Geoffrey Lomax, California Institute for Regenerative Medicine
• Melissa J. Lopes, Harvard University Embryonic Stem Cell Research Oversight Committee
• P. Pearl O’Rourke, Harvard Medical School
• Brendan Parent, NYU Grossman School of Medicine
• Steven Peckman, University of California, Los Angeles
• Monika Piotrowska, State University of New York at Albany
• May Schwarz, The Salk Institute for Biological Studies
• Jeff Sebo, New York University
• Chris Stodgell, University of Rochester
• Robert Streiffer, University of Wisconsin-Madison
• Lorenz Studer, Memorial Sloan Kettering Cancer Center
• Amy Wilkerson, The Rockefeller University

Here’s a link to and a citation for the report,

Creating Chimeric Animals: Seeking Clarity on Ethics and Oversight edited by Karen J. Maschke, Margaret M. Matthews, Kaitlynn P. Craig, Carolyn P. Neuhaus, Insoo Hyun, Josephine Johnston, The Hastings Center Report Volume 52, Issue S2 (Special Report), November‐December 2022 First Published: 09 December 2022

This report is open access.

Seeing beneath the surface; ancient Roman revealed in wall painting at the Louvre

Here’s a fascinating tale about art and hidden mysteries told at the 245th meeting of the American Chemical Society (ACS) taking place Apr. 7 – 11, 2013, from the Apr. 10, 2013 news release on EurekAlert,

J. Bianca Jackson, Ph.D., who reported on the project, explained that it involved a fresco [located at the Louvre Museum in Paris], which is a mural or painting done on a wall after application of fresh plaster. In a fresco, the artist’s paint seeps into the wet plaster and sets as the plaster dries. The painting becomes part of the wall. The earliest known frescoes date to about 1500 B.C. and were found on the island of Crete in Greece.

“No previous imaging technique, including almost half a dozen commonly used to detect hidden images below paintings, forged signatures of artists and other information not visible on the surface has revealed a lost image in this fresco,” Jackson said. “This opens to door to wider use of the technology in the world of art, and we also used the method to study a Russian religious icon and the walls of a mud hut in one of humanity’s first settlements in what was ancient Turkey.”

Here’s the technology they used to discover the figure hidden in the fresco,

… Termed terahertz spectroscopy, it uses beams of electromagnetic radiation that lie between microwaves, like those used in kitchen ovens, and the infrared rays used in TV remote controls. This radiation is relatively weak, does not damage paintings and does not involve exposure to harmful radiation.

“Terahertz technology has been in use for some time, especially in quality control in the pharmaceutical industry to assure the integrity of pills and capsules, in biomedical imaging and even in homeland security with those whole-body scanners that see beneath clothing at airport security check points,” said Jackson, who is now with the University of Rochester. “But its use in examining artifacts and artworks is relatively new.”

The scientists turned to terahertz technology when suspicions surfaced that a hidden image might lie beneath the brushstrokes of a precious 19th century fresco, Trois hommes armés de lances, in the Louvre’s Campana collection. …

To search for a hidden image, Jackson and colleagues, including Gerard Mourou, Ph.D., of Ècole Polytechnique, and Michel Menu, Ph.D., of the Centre de Recherche et de Restauration des Musées de France, and Vincent Detalle, of the Laboratoire Recherche des Monuments Historiques, probed it with terahertz technology. The process is slow, requiring a few hours to analyze a section the size of a sheet of paper.

“We were amazed, and we were delighted,” said Jackson. “We could not believe our eyes as the image materialized on the screen. Underneath the top painting of the folds of a man’s tunic, we saw an eye, a nose and then a mouth appear. We were seeing what likely was part of an ancient Roman fresco, thousands of years old.”

Who is the man in the fresco? An imperial Roman senator? A patrician? A plebian? A great orator? A ruler who changed the course of history? Or just a wealthy, egotistical landowner who wanted to admire his image on the wall?

Jackson is leaving those questions to art historians.

For anyone interested in Campana,

Giampietro Campana was an Italian art collector in the 1800s whose treasures are now on display in museums around the world. When Campana acquired a work of art, he sometimes restored damaged parts or reworked the original. Art historians believe that Campana painted Trois hommes armés de lances after the fresco was removed from its original wall in Italy and entered his collection.

Campana’s practice of restoring and reworking the original was not unusual for the time,

Artists, including some of the great masters, sometimes re-used canvases, wiping out the initial image or covered old paintings with new works. They often did this in order to avoid the expense of buying a new canvas or to enhance colors and shapes in a prior composition. Frescoes likewise got a refresh, especially when the originals faded, owners tired of the image on the wall or property changed hands.

This project was funded in part by CHARISMA [Cultural Heritage Advanced Research Infrastructure; Synergy for a Multidisciplinary Approach to Conservation/Restoration] as part of the European Union’s Framework Programme 7 (FP 7). This project called to mind the NanoForArt FP7 funded project I mentioned in the context of a Mar. 1, 2013 posting about cave art, frescos, and other examples of rock art and how nanotechnology is enabling conservation and restoration.

In any event, it’s nice to find out that those airport scanners are good for something other than delaying your trip and subjecting you and your knickers to inspection.