Tag Archives: vaccine

Possible nanoparticle-based vaccine/microbiocide for herpes simplex virus-2

An April 27, 2016 news item on ScienceDaily describes a new therapeutic and preventative technology for herpes,

An effective vaccine against the virus that causes genital herpes has evaded researchers for decades. But now, researchers from the University of Illinois at Chicago [UIC] working with scientists from Germany have shown that zinc-oxide nanoparticles shaped like jacks can prevent the virus from entering cells, and help natural immunity to develop.

“We call the virus-trapping nanoparticle a microbivac, because it possesses both microbicidal and vaccine-like properties,” says corresponding author Deepak Shukla, professor of ophthalmology and microbiology & immunology in the UIC College of Medicine. “It is a totally novel approach to developing a vaccine against herpes, and it could potentially also work for HIV and other viruses,” he said.

The particles could serve as a powerful active ingredient in a topically-applied vaginal cream that provides immediate protection against herpes virus infection while simultaneously helping stimulate immunity to the virus for long-term protection, explained Shukla.

An April 27, 2016 UIC news release (also on EurekAlert), which originated the news item, provides more context for the work,

Herpes simplex virus-2, which causes serious eye infections in newborns and immunocompromised patients as well as genital herpes, is one of the most common human viruses. According to the Centers for Disease Control and Prevention, about 15 percent of people from ages 14-49 carry HSV-2, which can hide out for long periods of time in the nervous system. The genital lesions caused by the virus increase the risk for acquiring human immunodeficiency virus, or HIV.

“Your chances of getting HIV are three to four times higher if you already have genital herpes, which is a very strong motivation for developing new ways of preventing herpes infection,” Shukla said.

Treatments for HSV-2 include daily topical medications to suppress the virus and shorten the duration of outbreaks, when the virus is active and genital lesions are present. However, drug resistance is common, and little protection is provided against further infections. Efforts to develop a vaccine have been unsuccessful because the virus does not spend much time in the bloodstream, where most traditional vaccines do their work.

The news release goes on to provide technical details,

The tetrapod-shaped zinc-oxide nanoparticles, called ZOTEN, have negatively charged surfaces that attract the HSV-2 virus, which has positively charged proteins on its outer envelope. ZOTEN nanoparticles were synthesized using technology developed by material scientists at Germany’s Kiel University and protected under a joint patent with UIC.

When bound to the nanoparticles, HSV-2 cannot infect cells. But the bound virus remains susceptible to processing by immune cells called dendritic cells that patrol the vaginal lining. The dendritic cells “present” the virus to other immune cells that produce antibodies. The antibodies cripple the virus and trigger the production of customized killer cells that identify infected cells and destroy them before the virus can take over and spread.

The researchers showed that female mice swabbed with HSV-2 and an ointment containing ZOTEN had significantly fewer genital lesions than mice treated with a cream lacking ZOTEN. Mice treated with ZOTEN also had less inflammation in the central nervous system, where the virus can hide out.

The researchers were able to watch immune cells pry the virus off the nanoparticles for immune processing, using high-resolution fluorescence microscopy.

“It’s very clear that ZOTEN facilitates the development of immunity by holding the virus and letting the dendritic cells get to it,” Shukla said.

If found safe and effective in humans, a ZOTEN-containing cream ideally would be applied vaginally just prior to intercourse, Shukla said. But if a woman who had been using it regularly missed an application, he said, she may have already developed some immunity and still have some protection. Shukla hopes to further develop the nanoparticles to work against HIV, which like HSV-2 also has positively charged proteins embedded in its outer envelope.

ZOTEN particles are uniform in size and shape, making them attractive for use in other biomedical applications. The novel flame transport synthesis technology used to make them allows large-scale production, said Rainer Adelung, professor of nanomaterials at Kiel University. And, because no chemicals are used, the production process is green.

Adelung hopes to begin commercial production of ZOTEN through a startup company that will be run jointly with his colleagues at UIC.

Here’s an image of the particles, courtesy of UIC,

Zinc oxide tetrapod nanoparticles. Credit: Deepak Shukla

Zinc oxide tetrapod nanoparticles. Credit: Deepak Shukla

Here’s a link to and a citation for the paper,

Intravaginal Zinc Oxide Tetrapod Nanoparticles as Novel Immunoprotective Agents against Genital Herpes by Thessicar E. Antoine, Satvik R. Hadigal, Abraam M. Yakoub, Yogendra Kumar Mishra, Palash Bhattacharya, Christine Haddad, Tibor Valyi-Nagy, Rainer Adelung, Bellur S. Prabhakar, and Deepak Shukla. The Journal of Immunology April 27, 2016 1502373  doi: 10.4049/jimmunol.1502373 Published online before print April 27, 2016

This paper is behind a paywall.

One final comment, it’s a long from a mouse vagina in this study to a human one.

Vaccines that are pure gold give patients breathing space

I exaggerated a little bit, the vaccine isn’t pure gold but it does have gold nanoparticles which mimic a virus. From the June 25, 2013 news item on ScienceDaily,

Scientists in the US have developed a novel vaccination method that uses tiny gold particles to mimic a virus and carry specific proteins to the body’s specialist immune cells.

The technique differs from the traditional approach of using dead or inactive viruses as a vaccine and was demonstrated in the lab using a specific protein that sits on the surface of the respiratory syncytial virus (RSV).

The results have been published today, 26 June [2013], in IOP Publishing’s journal Nanotechnology by a team of researchers from Vanderbilt University.

The June 26, 2013 IOP [Institute of Physics] Publishing news release (perhaps the journal publishers posted their news release after it was published elsewhere?), which originated the news item, provides more details about RSV and the technique,

RSV is the leading viral cause of lower respiration tract infections, causing several hundred thousand deaths and an estimated 65 million infections a year, mainly in children and the elderly.

The detrimental effects of RSV come, in part, from a specific protein, called the F protein, which coats the surface of the virus. The protein enables the virus to enter into the cytoplasm of cells and also causes cells to stick together, making the virus harder to eliminate.

The body’s natural defence to RSV is therefore directed at the F protein; however, up until now, researchers have had difficulty creating a vaccine that delivers the F protein to the specialised immune cells in the body. If successful, the F protein could trigger an immune response which the body could ‘remember’ if a subject became infected with the real virus.

In this study the researchers created exceptionally small gold nanorods, just 21 nanometres wide and 57 nanometres long, which were almost exactly the same shape and size as the virus itself. The gold nanorods were successfully coated with the RSV F proteins and were bonded strongly thanks to the unique physical and chemical properties of the nanorods themselves.

The researchers then tested the ability of the gold nanorods to deliver the F protein to specific immune cells, known as dendritic cells, which were taken from adult blood samples.

Dendritic cells function as processing cells in the immune system, taking the important information from a virus, such as the F protein, and presenting it to cells that can perform an action against them―the T cells are just one example of a cell that can take action.

Once the F protein-coated nanorods were added to a sample of dendritic cells, the researchers analysed the proliferation of T cells as a proxy for an immune response. They found that the protein-coated nanorods caused the T cells to proliferate significantly more compared to non-coated nanorods and just the F protein alone.

Not only did this prove that the coated-nanorods were capable of mimicking the virus and stimulating an immune response, it also showed that they were not toxic to human cells, offering significant safety advantages and increasing their potential as a real-life human vaccine.

Lead author of the study, Professor James Crowe, said: “A vaccine for RSV, which is the major cause of viral pneumonia in children, is sorely needed. This study shows that we have developed methods for putting RSV F protein into exceptionally small particles and presenting it to immune cells in a format that physically mimics the virus. Furthermore, the particles themselves are not infectious.”

Due to the versatility of the gold nanorods, Professor Crowe believes that their potential use is not limited to RSV.

“This platform could be used to develop experimental vaccines for virtually any virus, and in fact other larger microbes such as bacteria and fungi.

“The studies we performed showed that the candidate vaccines stimulated human immune cells when they were interacted in the lab. The next steps to testing would be to test whether or not the vaccines work in vivo” Professor Crowe continued.

I look forward to hearing more about this new vaccine as they continue with the testing. Meanwhile, here’s a link to and a citation for the latest published work,

Gold nanorod vaccine for respiratory syncytial virus by John W Stone, Natalie J Thornburg, David L Blum, Sam J Kuhn, David W Wright, and James E Crowe Jr. Nanotechnology Volume 24 Number 29 or Nanotechnology 24 295102 doi:10.1088/0957-4484/24/29/295102

The article is open access.

Math, science and the movies; research on the African continent; diabetes and mice in Canada; NANO Magazine and Canada; poetry on Bowen Island, April 17, 2010

About 10 years ago, I got interested in how the arts and sciences can inform each other when I was trying to organize an art/science event which never did get off the ground (although I still harbour hopes for it one day).  It all came back to me when I read Dave Bruggeman’s (Pasco Phronesis blog) recent post about a new Creative Science Studio opening at the School of Cinematic Arts at the University of Southern California (USC). From Dave’s post,

It [Creative Science Studio] will start this fall at USC, where its School of Cinematic Arts makes heavy use of its proximity to Hollywood, and builds on its history of other projects that use science, technology and entertainment in other areas of research.

The studio will not only help studios improve the depiction of science in the products of their students, faculty and alumni (much like the Science and Entertainment Exchange), but help scientists create entertaining outreach products. In addition, science and engineering topics will be incorporated into the School’s curriculum and be supported in faculty research.

This announcement reminds me a little bit of an IBM/USC initiative in 2008 (from the news item on Nanowerk),

For decades Hollywood has looked to science for inspiration, now IBM researchers are looking to Hollywood for new ideas too.

The entertainment industry has portrayed possible future worlds through science fiction movies – many created by USC’s famous alumni – and IBM wants to tap into that creativity.

At a kickoff event at the USC School of Cinematic Arts, five of IBM’s top scientists met with students and alumni of the school, along with other invitees from the entertainment industry, to “Imagine the World in 2050.” The event is the first phase of an expected collaboration between IBM and USC to explore how combining creative vision and insight with science and technology trends might fuel novel solutions to the most pressing problems and opportunities of our time.

It’s interesting to note that the inspiration is two-way if the two announcements are taken together. The creative people can have access to the latest science and technology work for their pieces and scientists can explore how an idea or solution to a problem that exists in a story might be made real.

I’ve also noted that the first collaboration mentioned suggests that the Creative Science Studio will be able to “help scientists create entertaining outreach products.” My only caveat is that scientists too often believe that science communication means that they do all the communicating while we members of the public are to receive their knowledge enthusiastically and uncritically.

Moving on to the math that I mentioned in the head, there’s an announcement of a new paper that discusses the use of mathematics in cinematic special effects. (I believe that the word cinematic is starting to include games and other media in addition to movies.)  From the news item on physorg.com,

The use of mathematics in cinematic special effects is described in the article “Crashing Waves, Awesome Explosions, Turbulent Smoke, and Beyond: Applied Mathematics and Scientific Computing in the Visual Effects Industry”, which will appear in the May 2010 issue of the NOTICES OF THE AMS [American Mathematical Society]. The article was written by three University of California, Los Angeles, mathematicians who have made significant contributions to research in this area: Aleka McAdams, Stanley Osher, and Joseph Teran.

Mathematics provides the language for expressing physical phenomena and their interactions, often in the form of partial differential equations. These equations are usually too complex to be solved exactly, so mathematicians have developed numerical methods and algorithms that can be implemented on computers to obtain approximate solutions. The kinds of approximations needed to, for example, simulate a firestorm, were in the past computationally intractable. With faster computing equipment and more-efficient architectures, such simulations are feasible today—and they drive many of the most spectacular feats in the visual effects industry.

This news item too brought back memories. There was a Canadian animated film, Ryan, which both won an Academy Award and involved significant collaboration between a mathematician and an animator. From the MITACS (Mathematics of Information Technology and Complex Systems)  2005 newsletter, Student Notes:

Karan Singh is an Associate Professor at the University of Toronto, where co-directs the graphics and HCI lab, DGP. His research interests are in artist driven interactive graphics encompassing geometric modeling, character animation and non-photorealistic rendering. As a researcher at Alias (1995-1999), he architected facial and character animation tools for Maya (Technical Oscar 2003). He was involved with conceptual design and reverse engineering software at Paraform (Academy award for technical achievement 2001) and currently as Chief Scientist for Geometry Systems Inc. He has worked on numerous film and animation projects and most recently was the R+D Director for the Oscar winning animation Ryan (2005)

Someone at Student Notes (SN) goes on to interview Dr. Singh (here’s an excerpt),

SN: Some materials discussing the film Ryan mention the term “psychorealism”. What does this term mean? What problems does the transition from realism to psychorealism pose for the animator, or the computer graphics designer?

KS: Psychorealism is a term coined by Chris {Landreth, film animator] to refer to the glorious complexity of the human psyche depicted through the visual medium of art and animation. The transition is not a problem, psychorealism is stylistic, just a facet to the look and feel of an animation. The challenges lies in the choice and execution of the metaphorical imagery that the animator makes.

Both the article and Dr. Singh’s page are well worth checking out, if the links between mathematics and visual imagery interest you.

Research on the African continent

Last week I received a copy of Thompson Reuters Global Research Report Africa. My hat’s off to the authors, Jonathan Adams, Christopher King, and Daniel Hook for including the fact that Africa is a continent with many countries, many languages, and many cultures. From the report, (you may need to register at the site to gain access to it but the only contact I ever get is a copy of their newsletter alerting me to a new report and other incidental info.), p. 3,

More than 50 nations, hundreds of languages, and a welter of ethnic and cultural diversity. A continent possessed of abundant natural resources but also perennially wracked by a now-familiar litany of post-colonial woes: poverty, want, political instability and corruption, disease, and armed conflicts frequently driven by ethnic and tribal divisions but supplied by more mature economies. OECD’s recent African Economic Outlook sets out in stark detail the challenge, and the extent to which current global economic problems may make this worse …

While they did the usual about challenges, the authors go on to add this somewhat contrasting information.

Yet the continent is also home to a rich history of higher education and knowledge creation. The University of Al-Karaouine, at Fez in Morocco, was founded in CE 859 as a madrasa and is identified by many as the oldest degree-awarding institution in the world.ii It was followed in 970 by Al-Azhar University in Egypt. While it was some centuries before the curriculum expanded from religious instruction into the sciences this makes a very early marker for learning. Today, the Association of African Universities lists 225 member institutions in 44 countries and, as Thomson Reuters data demonstrate, African research has a network of ties to the international community.

A problem for Africa as a whole, as it has been for China and India, is the hemorrhage of talent. Many of its best students take their higher degrees at universities in Europe, Asia and North America. Too few return.

I can’t speak for the details included in the report which appears to be a consolidation of information available in various reports from international organizations. Personally, I find these consolidations very helpful as I would never have the time to track all of this down. As well, they have created a graphic which illustrates research relationships. I did have to read the analysis in order to better understand the graphic but I found the idea itself quite engaging and as I can see (pun!) that as one gets more visually literate with this type of graphic that it could be a very useful tool for grasping complex information very quickly.

Diabetes and mice

Last week, I missed this notice about a Canadian nanotechnology effort at the University of Calgary. From the news item on Nanowerk,

Using a sophisticated nanotechnology-based “vaccine,” researchers were able to successfully cure mice with type 1 diabetes and slow the onset of the disease in mice at risk for the disease. The study, co-funded by the Juvenile Diabetes Research Foundation (JDRF), provides new and important insights into understanding how to stop the immune attack that causes type 1 diabetes, and could even have implications for other autoimmune diseases.

The study, conducted at the University of Calgary in Alberta, Canada, was published today [April 8, 2010?] in the online edition of the scientific journal Immunity.

NANO Magazine

In more recent news, NANO Magazine’s new issue (no. 17) features a country focus on Canada. From the news item on Nanowerk,

In a special bumper issue of NANO Magazine we focus on two topics – textiles and nanomedicine. We feature articles about textiles from Nicholas Kotov and Kay Obendorf, and Nanomedicine from the London Centre for Nanotechnology and Hans Hofstraat of Philips Healthcare and an interview with Peter Singer, NANO Magazine Issue 17 is essential reading, www.nanomagazine.co.uk.

The featured country in this issue is Canada [emphasis mine], notable for its well funded facilities and research that is aggressively focused on industrial applications. Although having no unifying national nanotechnology initiative, there are many extremely well-funded organisations with world class facilities that are undertaking important nano-related research.

I hope I get a chance to read this issue.

Poetry on Bowen Island

Heather Haley, a local Vancouver, BC area, poet is hosting a special event this coming Saturday at her home on Bowen Island. From the news release,

VISITING POETS Salon & Reading

Josef & Heather’s Place
Bowen Island, BC
7:30  PM
Saturday, April 17, 2010

PENN KEMP, inimitable sound poet from London, Ontario

The illustrious CATHERINE OWEN from Vancouver, BC

To RSVP and get directions please email hshaley@emspace.com

Free Admission
Snacks & beverages-BYOB

Please come on over to our place on the sunny south slope to welcome these fabulous poets, hear their marvelous work, *see* their voices right here on Bowen Island!

London, ON performer and playwright PENN KEMP has published twenty-five books of poetry and drama, had six plays and ten CDs produced as well as Canada’s first poetry CD-ROM and several videopoems.  She performs in festivals around the world, most recently in Britain, Brazil and India. Penn is the Canada Council Writer-in-Residence at UWO for 2009-10.  She hosts an eclectic literary show, Gathering Voices, on Radio Western, CHRWradio.com/talk/gatheringvoices.  Her own project for the year is a DVD devoted to Ecco Poetry, Luminous Entrance: a Sound Opera for Climate Change Action, which has just been released.
CATHERINE OWEN is a Vancouver writer who will be reading from her latest book Frenzy (Anvil Press 09) which she has just toured across the entirety of Canada. Her work has appeared in international magazines, seen translation into three languages and been nominated for honours such as the BC Book Prize and the CBC Award. She plays bass and sings in a couple of metal bands and runs her own tutoring and editing business.

I have seen one of Penn Kemp’s video poems. It was at least five years ago and it still resonates with me . Guess what? I highly recommend going if you can. If you’re curious about Heather and her work, go here.