Monthly Archives: June 2024

Grow inorganic functional nanomaterials—quantum dots—in the nucleus of live cells

I’m not sure that “transform[ing] cells into super cells, enabling them to do unimaginable thing,” as research Pang Dai-Wen says, is something that is necessary but he and at least one of his colleagues seem quite enthused by the prospect (you’ll find Pang’s quote in the press release which follows the news item).

An April 3, 2024 news item on phys.org announces the work, Note: Links have been removed,

National Science Review recently published research on the synthesis of quantum dots (QDs) in the nucleus of live cells by Dr. Hu Yusi, Associate Professor Wang Zhi-Gang, and Professor Pang Dai-Wen from Nankai University.

During the study of QDs synthesis in mammalian cells, it was found that the treatment with glutathione (GSH) enhanced the cell’s reducing capacity. The generated QDs were not uniformly distributed within the cell but concentrated in a specific area.

Through a series of experiments, it was confirmed that this area is indeed the cell nucleus. Dr. Hu said, “This is truly amazing, almost unbelievable.”

An April 3, 2024 Science China Press press release on EurekAlert, which originated the news item, fills in a few details,

Dr. Hu and his mentor Professor Pang attempted to elucidate the molecular mechanism of quantum dot synthesis in the cell nucleus. It was found that GSH plays a significant role. There is a GSH transport protein, Bcl-2, on the nucleus, which transports GSH into the nucleus in large quantities, enhancing the reducing ability within the nucleus, promoting the generation of Se precursors. At the same time, GSH can also expose thiol groups on proteins, creating conditions for the generation of Cd precursors. The combination of these factors ultimately enables the abundant synthesis of quantum dots in the cell nucleus.

Professor Pang stated, “This is an exciting result; this work achieves the precise synthesis of QDs in live cells at the subcellular level.” He continued, “Research in the field of synthetic biology mostly focuses on live cell synthesis of organic molecules through reverse genetics. Rarely do we see the live cell synthesis of inorganic functional materials. Our study doesn’t involve complex genetic modifications; it achieves the target synthesis of inorganic fluorescent nanomaterials in cellular organelles simply by regulating the content and distribution of GSH within the cell. This addresses the deficiency in synthetic biology for the synthesis of inorganic materials.”

While the synthesis of organic materials in cells remains predominant in the field of biosynthesis, this research undoubtedly paves the way for the synthesis of inorganic materials in synthetic biology. Professor Pang expressed, “Each of our advancements is a new starting point. We firmly believe that in the near future, we can use cell synthesis to produce nanodrugs, or even nanorobots in specified organelles. Moreover, we can transform cells into super cells, enabling them to do unimaginable things.” [emphasis mine]

Here’s a link to and a citation for the paper,

In-situ synthesis of quantum dots in the nucleus of live cells by Yusi Hu, Zhi-Gang Wang, Haohao Fu, Chuanzheng Zhou, Wensheng Cai, Xueguang Shao, Shu-Lin Liu , Dai-Wen Pang. National Science Review, Volume 11, Issue 3, March 2024, nwae021, DOI: https://doi.org/10.1093/nsr/nwae021 Published:: 12 January 2024

This paper appears to be open access.

Pulp and paper waste for scrubbing carbon from emissions

This first news release is a little short but the next one is one of the shortest I can recall seeing. First, a February 1, 2024 Canadian Light Source (CLS) news release by Victoria Martinez,

Researchers at McGill University have come up with an innovative approach to improve the energy efficiency of carbon conversion, using waste material from pulp and paper production. The technique they’ve pioneered using the Canadian Light Source at the University of Saskatchewan not only reduces the energy required to convert carbon into useful products, but also reduces overall waste in the environment.

“This is a new field,” says Roger Lin, a graduate student in chemical engineering “We are one of the first groups to combine biomass recycling or utilization with CO2 capture.” The research team, from McGill’s Electrocatalysis Lab, published their findings in the journal RSC [Royal Society of Chemistry] Sustainability.

Capturing carbon emissions is one of the most exciting emerging tools to fight climate change. The biggest challenge is figuring out what to do with the carbon once the emissions have been removed, especially since capturing CO2 can be expensive. The next hurdle is that transforming CO2 into useful products takes energy. Researchers want to make the conversion process as efficient and profitable as possible.

“For these reactions, it really matters that we target energy efficiency,” says Amirhossein Farzi, a PhD student in chemical engineering at McGill. “The highest burden on the profitability of these reactions and these processes is usually how energy efficient they are.”

Farzi, Lin, and their research team focused their efforts on changing out one of the most energy-intensive parts of the carbon conversion process.

Because the approach is so new, there are many questions to answer about how to get the purest outputs and best efficiency. The team used CLS beamlines to observe chemical reactions in real-time, mimicking industrial processes as closely as possible.

The researchers hope to expand the range of products that can be made with CO2, and help develop a truly green technology.

“If we use a renewable energy source like hydro, wind, or solar …then in the end, we have really a carbon negative process,” says Lin.

Then, there was a March 27, 2024 McGill University news release (also on EurekAlert but published April 8, 2024), which is more succinct,

Researchers at McGill University have come up with an innovative approach to improve the energy efficiency of carbon conversion, using waste material from pulp and paper production. The technique they’ve pioneered using the Canadian Light Source at the University of Saskatchewan not only reduces the energy required to convert carbon into useful products, but also reduces overall waste in the environment.

“We are one of the first groups to combine biomass recycling or utilization with CO2 capture,” said Ali Seifitokaldani, Assistant Professor in the Department of Chemical Engineering and Canada Research Chair (Tier II) in Electrocatalysis for Renewable Energy Production and Conversion. The research team, from McGill’s Electrocatalysis Lab, published their findings in the journal RSC Sustainability.

Capturing carbon emissions is one of the most exciting emerging tools to fight climate change. The biggest challenge is figuring out what to do with the carbon once the emissions have been removed, especially since capturing CO2 can be expensive. The next hurdle is that transforming CO2 into useful products takes energy. Researchers want to make the conversion process as efficient and profitable as possible.

Here’s a link to and a citation for the paper,

Efficient integration of carbon dioxide reduction and 5-hydroxymethylfurfural oxidation at high current density by Roger Lin, Haoyan Yang, Hanyu Zheng, Mahdi Salehi, Amirhossein Farzi, Poojan Patel, Xiao Wang, Jiaxun Guo, Kefang Liu, Zhengyuan Gao, Xiaojia Li, Ali Seifitokaldani. RSC Sustainability, 2024; 2 (2): 445 DOI: 10.1039/D3SU00379E First published online: 13 Dec 2023

This paper is open access.

H/t April 8, 2024 news item on ScienceDaily

Grown from bacteria: plastic-free vegan leather that dyes itself

Interesting rather than aesthetiically pleasing,

Caption: Bacteria grown and dyed shoe. Credit; Tom Ellis/Marcus Walker/Imperial College London

An April 3, 2024 news item on phys.org announces this latest example of bacterial footwear,

Researchers at Imperial College London have genetically engineered bacteria to grow animal- and plastic-free leather that dyes itself.

In recent years, scientists and companies have started using microbes to grow sustainable textiles or to make dyes for industry—but this is the first time bacteria have been engineered to produce a material and its own pigment simultaneously.

An April 3, 2024 Imperial College London (ICL) press release (also on EurekAlert) by Caroline Brogan, which originated the news item, delves further into the research, Note: Links have been removed,

Synthetic chemical dyeing is one of the most environmentally toxic processes in fashion, and black dyes – especially those used in colouring leather – are particularly harmful. The researchers at Imperial set out to use biology to solve this.

In tackling the problem, the researchers say their self-dyeing vegan, plastic-free leather, which has been fashioned into shoe and wallet prototypes, represents a step forward in the quest for more sustainable fashion.

Their new process, which has been published in the journal Nature Biotechnology, could also theoretically be adapted to have bacteria grow materials with various vibrant colours and patterns, and to make more sustainable alternatives to other textiles such as cotton and cashmere.

Lead author Professor Tom Ellis, from Imperial College London’s Department of Bioengineering, said: “Inventing a new, faster way to produce sustainable, self-dyed leather alternatives is a major achievement for synthetic biology and sustainable fashion.

“Bacterial cellulose is inherently vegan, and its growth requires a tiny fraction of the carbon emissions, water, land use and time of farming cows for leather.

“Unlike plastic-based leather alternatives, bacterial cellulose can also be made without petrochemicals, and will biodegrade safely and non-toxically in the environment.”

Designer collaboration

The researchers created the self-dyeing leather alternative by modifying the genes of a bacteria species that produces sheets of microbial cellulose – a strong, flexible and malleable material that is already commonly used in food, cosmetics and textiles. The genetic modifications ‘instructed’ the same microbes that were growing the material to also produce the dark black pigment, eumelanin.

They worked with designers to grow the upper part of a shoe (without the sole) by growing a sheet of bacterial cellulose in a bespoke, shoe-shaped vessel. After 14 days of growth wherein the cellulose took on the correct shape, they subjected the shoe to two days of gentle shaking at 30°C to activate the production of black pigment from the bacteria so that it dyed the material from the inside.

They also made a black wallet by growing two separate cellulose sheets, cutting them to size, and sewing them together.

As well as the prototypes, the researchers demonstrated that the bacteria can be engineered using genes from other microbes to produce colours in response to blue light. By projecting a pattern, or logo, onto the sheets using blue light, the bacteria respond by producing coloured proteins which then glow.

This allows them to project patterns and logos onto the bacterial cultures as the material grows, resulting in patterns and logos forming from within the material. 

Co-author Dr Kenneth Walker, who conducted the work at Imperial College London’s Department of Bioengineering and now works in industry, said: “Our technique works at large enough scales to create real-life products, as shown by our prototypes. From here, we can consider aesthetics as well as alternative shapes, patterns, textiles, and colours.

“The work also shows the impact that can happen when scientists and designers work together. As current and future users of new bacteria-grown textiles, designers have a key role in championing exciting new materials and giving expert feedback to improve form, function, and the switch to sustainable fashion.”

Greener clothes

The research team are now experimenting with a variety of coloured pigments to use those that can also be produced by the material-growing microbes.

The researchers and collaborators have also just won £2 million in funding from Biotechnology and Biological Sciences Research Council (BBSRC), part of UK Research and Innovation (UKRI), to use engineering biology and bacterial cellulose to solve more of fashion’s problems, such as the use of toxic chromium in leather’s production lines.

Professor Ellis said: “Microbes are already directly addressing many of the problems of animal and plastic-based leather, and we plan to get them ready to expand into new colours, materials and maybe patterns too.

“We look forward to working with the fashion industry to make the clothes we wear greener throughout the whole production line.”

The authors worked closely with Modern Synthesis, a London-based biodesign and materials company, who specialise in innovative microbial cellulose products.

This work was funded by Engineering and Physical Sciences Research Council and BBSRC, both part of UKRI.

Here’s a link to and a citation for the paper,

Self-pigmenting textiles grown from cellulose-producing bacteria with engineered tyrosinase expression by Kenneth T. Walker, Ivy S. Li, Jennifer Keane, Vivianne J. Goosens, Wenzhe Song, Koon-Yang Lee & Tom Ellis. Nature Biotechnology (2024) DOI: https://doi.org/10.1038/s41587-024-02194-3 Published: 02 April 2024

This paper is open access.

Modern Synthesis, the company with which the researchers collaborated, can be found here.

Fullertubes, at last!

A theoretical possibility has been proven by an international team including researchers from the Université de Montréal (University of Montreal) according to a March 27, 2024 news item on phys.org,

For years, C130 fullertubes—molecules made up of 130 carbon atoms—have existed only in theory. Now, leading an international team of scientists, a UdeM doctoral student in physics has successfully shown them in real life—and even managed to capture some in a photograph.

Illustration of the discovery of the C130-D5h molecule, published on the cover page of the prestigious “Journal of the American Chemical Society” last December. Credit: JACS

A March 27, 2024 Université de Montréal (UdeM) nouvelles (news release), which originated the news item, provides more detail, Note: Links have been removed,

This feat in the realm of basic research has led Emmanuel Bourret to have a cover-page illustration of his discovery in a prestigious scientific journal, the Journal of the American Chemical Society.

First published online last October [2023], the discovery was made by Bourret as lead scientist of an inter-university team that also included researchers from Purdue University, Virginia Tech and the Oak Ridge National Laboratory, in Tennessee.

A fullertube is basically an assembly of carbon atoms arranged to form a closed tubular cage. It is related to fullerenes, molecules that are represented as cages of interconnected hexagons and pentagons, and come in a wide variety of sizes and shapes.

For example, a C60 fullerene is made up of 60 carbon atoms and is shaped like a soccer ball. It is relatively small, spherical and very abundant. C120 fullerenes are less common. They are longer and shaped like a tube capped at either ends with the two halves of a C60 fullerene.

Found in soot

The C130 fullertube (or C130-D5h, its full scientific name) is more elongated than the C120 and even rarer. To isolate it, Bourret and his team generated an electric arc between two graphite electrodes to produce soot containing fullerene and fullertube molecules. The electronic structure of these molecules was then calculated using density functional theory (DFT).

“Drawing on principles of quantum mechanics, DFT enables us to calculate electronic structures and predict the properties of a molecule using the fundamental rules of physics,” explained Bourret’s thesis supervisor, UdeM physics professor Michel Côté, a researcher at the university’s Institut Courtois.

Using special software, Bourret was able to describe the structure of the C130 molecule: it is a tube with two hemispheres at the ends, making it look like a microscopic capsule. It measures just under 2 nanometres long by 1 nm wide (a nanometre is one billionth of a metre).

“The structure of the tube is basically made up of atoms arranged in hexagons,” said Bourret. “At the two ends, these hexagons are linked by pentagons, giving them their rounded shape.”

Bourret began doing theoretical work on fullertubes in 2014 under his then-supervisor Jiri Patera, an UdeM mathematics professor. After Patera passed away in January 2022, Bourret then approached Côté, who became his new supervisor.

Existence shown in 2020

Two years before that, Bourret had read an article by Purdue University at Fort Wayne professor Steven Stevenson, who described the experimental isolation of certain fullertubes, demonstrating their existence but not identifying all of them.

Under Côté’s guidance, Bourret set to work advancing knowledge on the topic.

“Emmanuel had a strong background in abstract mathematics,” Bourret recalled, “and he added an interesting dimension to my research group, which focuses on more computational approaches.”

Are any possible future applications in the offing?

“It’s hard to say at this stage, but one possibility might be the production of hydrogen,” said Côté. “Currently, what’s used is a catalyst made of platinum and rubidium, both of which are rare and expensive. Replacing them with carbon structures such as C130 would make it possible to produce hydrogen in a ‘greener’ way.”

Last year, Bourret’s groundbreaking work earned him an invitation to deliver a paper at the annual meeting of the U.S. Electrochemical Society (ECS), in Boston. This May [2024], he’ll chair a panel on fullerenes and fullertubes at the ECS annual meeting in San Francisco.

Here’s a link to and a citation for the paper,

Colossal C130 Fullertubes: Soluble [5,5] C130-D5h(1) Pristine Molecules with 70 Nanotube Carbons and Two 30-Atom Hemifullerene End-caps by Emmanuel Bourret, Xiaoyang Liu, Cora A. Noble, Kevin Cover, Tanisha P. Davidson, Rong Huang, Ryan M. Koenig, K. Shawn Reeves, Ivan V. Vlassiouk, Michel Côté, Jefferey S. Baxter, Andrew R. Lupini, David B. Geohegan, Harry C. Dorn, and Steven Stevenson. J. Am. Chem. Soc. 2023, 145, 48, 25942–25947 DOI: https://doi.org/10.1021/jacs.3c09082 Publication Date: October 27, 2023 Copyright © 2023 American Chemical Society

This paper is behind a paywall.

Why convert space data into sounds?

The Eagle Nebula (also known as M16 or the Pillars of Creation) was one of the 3 cosmic objects sonified and used in the study. Credit: X-ray: NASA/CXC/INAF/M.Guarcello et al.; Optical: NASA/STScI [downloaded from https://www.frontiersin.org/news/2024/03/25/communication-nasa-scientists-space-data-sounds]

Apparently, it’s all about communication or so a March 24, 2024 Frontiers news release (also on EurekAlert but published March 25, 2024) by Kim Arcand and Megan Watzke suggests, Note: Links have been removed,

Images from telescopes like the James Webb Space Telescope have expanded the way we see space. But what if you can’t see? Can stars be turned into sounds instead? In this guest editorial, NASA [US National Aeronautics and Space Administration] scientists and science communicators Dr Kimberly Arcand and Megan Watzke explain how and why they and their colleagues transformed telescope data into soundscapes to share space science with the whole world. To learn more, read their new research published in Frontiers in Communication.

When you travel somewhere where they speak a language you can’t understand, it’s usually important to find a way to translate what’s being communicated to you. In some ways, the same can be said about scientific data collected from cosmic objects. A telescope like NASA’s Chandra X-ray Observatory captures X-rays, which are invisible to the human eye, from sources across the cosmos. Similarly, the James Webb Space Telescope captures infrared light, also invisible to the human eye. These different kinds of light are transmitted down to Earth packed up in the form of ones and zeroes. From there, the data are transformed into a variety of formats — from plots to spectra to images.

This last category — images — is arguably what telescopes are best known for. For most of astronomy’s long history, however, most people who are blind or low vision (BLV) have not been able to fully experience the data that these telescopes have captured. NASA’s Universe of Sound data sonification program, with NASA’s Chandra X-ray Observatory and NASA’s Universe of Learning, translates visual data of objects in space into sonified data. All telescopes — including Chandra, Webb, the Hubble Space Telescope, plus dozens of others — in space need to send the data they collect back to Earth as binary code, or digital signals. Typically, astronomers and others turn these digital data into images, which are often spectacular and make their way into everything from websites to pillowcases.

The music of the spheres

By taking these data through another step, however, experts on this project mathematically map the information into sound. This data-driven process is not a reimagining of what the telescopes have observed, it is yet another kind of translation. Instead of a translation from French to Mandarin, it’s a translation from visual to sound. Releases from the Universe of Sound sonification project have been immensely popular with non-experts, from viral news stories with over two billion people potentially reached according to press metrics, to triple the usual Chandra.si.edu website traffic.

But how are such data sonifications perceived by people, particularly members of the BLV community? How do data sonifications affect participant learning, enjoyment, and exploration of astronomy? Can translating scientific data into sound help enable trust or investment, emotionally or intellectually, in scientific data? Can such sonifications help improve awareness of accessibility needs that others might have?

Listening closely

This study used our sonified NASA data of three astronomical objects. We surveyed blind or low-vision and sighted individuals to better understand participant experiences of the sonifications, relating to their enjoyment, understanding, and trust of the scientific data. Data analyses from 3,184 sighted or blind or low-vision participants yielded significant self-reported learning gains and positive experiential responses.

The results showed that astrophysical data engaging multiple senses like the sonifications could establish additional avenues of trust, increase access, and promote awareness of accessibility in sighted and blind or low-vision communities. In short, sonifications helped people access and engage with the Universe.

Sonification is an evolving and collaborative field. It is a project not only done for the BLV community, but with BLV partnerships. A new documentary available on NASA’s free streaming platform NASA+ explores how these sonifications are made and the team behind them. The hope is that sonifications can help communicate the scientific discoveries from our Universe with more audiences, and open the door to the cosmos just a little wider for everyone.

Here’s a link to and a citation for the paper,

A Universe of Sound: processing NASA data into sonifications to explore participant response by Kimberly Kowal Arcand, Jessica Sarah Schonhut-Stasik, Sarah G. Kane, Gwynn Sturdevant, Matt Russo, Megan Watzke, Brian Hsu, Lisa F. Smith. Front. Commun., 13 March 2024 Volume 9 – 2024 DOI: https://doi.org/10.3389/fcomm.2024.1288896

This paper is open access.

Wall paint that’s self-cleaning

Who hasn’t had dingy walls? It seems there may be a solution at some point in the future according to a March 25, 2024 news item on ScienceDaily,

Typically, beautiful white wall paint does not stay beautiful and white forever. Often, various substances from the air accumulate on its surface. This can be a desired effect because it makes the air cleaner for a while — but over time, the colour changes and needs to be renewed.

A research team from TU Wien [Vienna University of Technology] and the Università Politecnica delle Marche (Italy) has now succeeded in developing special titanium oxide nanoparticles that can be added to ordinary, commercially available wall paint to establish self-cleaning power: The nanoparticles are photocatalytically active, they can use sunlight not only to bind substances from the air, but also to decompose them afterwards. The wall makes the air cleaner — and cleans itself at the same time. Waste was used as the raw material for the new wall paint: metal scrap, which would otherwise have to be discarded, and dried fallen leaves.

A March 25, 2024 Vienna University of Technology press release (also on EurekAlert), which originated the news item, describes the proposed technology,

Modified titanium oxide in the wall paint

A wide variety of pollutants occur in indoor air – from residues of cleaning agents and hygiene products to molecules that are produced during cooking or that are emitted by materials such as leather. In some cases, this can lead to health issues, which is then referred to as “sick building syndrome”.

“For years, people have been trying to use customized wall paints to clean the air,” says Prof. Günther Rupprechter from the Institute of Materials Chemistry at TU Wien. “Titanium oxide nanoparticles are particularly interesting in this context. They can bind and break down a wide range of pollutants.”

However, simply adding ordinary titanium oxide nanoparticles to the paint will affect the durability of the paint: just as pollutants are degraded by the nanoparticles, they can also make the paint itself unstable and create cracks. In the worst case, volatile organic compounds can even be released, which in turn can be harmful to health. After a certain time, the paint layer becomes gray and tinted, finally it has to be renewed.

Self-cleaning by light

However, the nanoparticles can clean themselves if they are irradiated with UV light. Titanium oxide is a so-called photocatalyst – a material that enables chemical reactions when exposed to suitable light. The UV radiation creates free charge carriers in the particles, which induce decomposition of the trapped pollutants from air into small parts and their release. In this way, the pollutants are rendered harmless, but do not remain permanently attached to the wall paint. The wall colour remains stable in the long term.

In practice, however, this is of little use – after all, it would be tedious to repeatedly irradiate the wall with intense UV light in order to drive the self-cleaning process. “Our goal was therefore to modify these particles in such a way that the photocatalytic effect can also be induced by ordinary sunlight,” explains Günther Rupprechter.

This is achieved by adding certain additional atoms to the titanium oxide nanoparticles, such as phosphorus, nitrogen, and carbon. As a result, the light frequencies that can be harvested by the particles change, and instead of just UV light, photocatalysis is then also triggered by ordinary visible light.

96% pollutant removal

“We have now investigated this phenomenon in great detail using a variety of different surface and nanoparticle analysis methods,” says Qaisar Maqbool, the first author of the study. “In this way, we were able to show exactly how these particles behave, before and after they were added to the wall paint.”

The research team mixed the modified titanium oxide nanoparticles with ordinary, commercially available wall paint and rinsed a painted surface with a solution containing pollutants. Subsequently, 96% of the pollutants could be degraded by natural sunlight. The colour itself does not change – because the pollutants are not only bound, but also broken down with the help of sunlight.

Waste as a raw material

For the commercial success of such paints, it is also important to avoid expensive raw materials . “In catalysis, for example, precious metals such as platinum or gold are used. In our case, however, elements that are readily available from everywhere are sufficient: To obtain phosphorus, nitrogen and carbon, we have used dried fallen leaves from olive trees, and the titanium for the titanium oxide nanoparticles was obtained from metal waste, which is normally simply thrown away,” says Günther Rupprechter.

This new type of wall paint combines several advantages at the same time: it removes pollutants from the air, it lasts longer than other paints – and it is even more resource-saving in production as it can be obtained from recycled materials. Further experiments are being carried out, and commercialisation of the wall paint is intended.

Here’s a link to and a citation for the paper,

Highly Stable Self-Cleaning Paints Based on Waste-Valorized PNC-Doped TiO2 Nanoparticles by Qaisar Maqbool, Orlando Favoni, Thomas Wicht, Niusha Lasemi, Simona Sabbatini, Michael Stöger-Pollach, Maria Letizia Ruello, Francesca Tittarelli, and Günther Rupprechter. ACS Catal. 2024, 14, 7, 4820–4834 DOI: https://doi.org/10.1021/acscatal.3c06203 Publication Date:March 15, 2024 Copyright © 2024 The Authors. Published by American Chemical Society. This publication is licensed under
CC-BY 4.0..

This paper is open access.

Tissue-like bioelectronic mesh system capable of growing with cardiac tissues

Graphene, heralded for its biocompatibility, features in a March 22, 2024 news item on ScienceDaily about research about biosensing and a mesh system that can grow,

A team of engineers has recently built a tissue-like bioelectronic mesh system integrated with an array of atom-thin graphene sensors that can simultaneously measure both the electrical signal and the physical movement of cells in lab-grown human cardiac tissue. This tissue-like mesh can grow along with the cardiac cells, allowing researchers to observe how the heart’s mechanical and electrical functions change during the developmental process. The new device is a boon for those studying cardiac disease as well as those studying the potentially toxic side-effects of many common drug therapies.

Caption: A bioelectronic mesh, studded with graphene sensors (red), can measure the electrical signal and movement of cardiac tissue (purple and green) at the same time. Credit: Gao et al., 10.1038/s41467-024-46636-7

A March 21, 2024 University of Massachusetts at Amherst news release (also on EurekAlert), which originated the news item, delves further into the topics of heart disease and biosensor implants, Note: Links have been removed,

Cardiac disease is the leading cause of human morbidity and mortality across the world. The heart is also very sensitive to therapeutic drugs, and the pharmaceutical industry spends millions of dollars in testing to make sure that its products are safe. However, ways to effectively monitor living cardiac tissue are extremely limited.

In part, this is because it is very risky to implant sensors in a living heart, but also because the heart is a complex kind of muscle with more than one thing that needs monitoring. “Cardiac tissue is very special,” says Jun Yao, associate professor of electrical and computer engineering in UMass Amherst’s College of Engineering and the paper’s senior author. “It has a mechanical activity—the contractions and relaxations that pump blood through our body—coupled to an electrical signal that controls that activity.”

But today’s sensors can typically only measure one characteristic at a time, and a two-sensor device that could measure both charge and movement would be so bulky as to impede the cardiac tissue’s function. Until now, there was no single sensor capable of measuring the heart’s dual properties without interfering with its functioning.

The new device is built of two critical components, explains lead author Hongyan Gao, who is pursuing his Ph.D. in electrical engineering at UMass Amherst. The first is a three-dimensional cardiac microtissue (CMT), grown in a lab from human stem cells under the guidance of co-author Yubing Sun, associate professor of mechanical and industrial engineering at UMass Amherst. CMT has become the preferred model for in vitro testing because it is the closest analog yet to a full-size, living human heart. However, because CMT is grown in a test tube, it has to mature, a process that takes time and can be easily disrupted by a clumsy sensor.

The second critical component involves graphene—a pure-carbon substance only one atom thick. Graphene has a few surprising quirks to its nature that make it perfect for a cardiac sensor. Graphene is electrically conductive, and so it can sense the electrical charges shooting through cardiac tissue. It is also piezoresistive, which means that as it is stretched—say, by the beating of a heart—its electrical resistance increases. And because graphene is impossibly thin, it can register even the tiniest flutter of muscle contraction or relaxation and can do so without impeding the heart’s function, all through the maturation process. Co-author Jing Kong, professor of electrical engineering at MIT, and her group supplied this critical graphene material.

“Although there have already been many applications for graphene, it is wonderful to see that it can be used in this critical need, which takes advantage of graphene’s different characteristics,” says Kong.

Gao, Yao and their colleagues then embedded a series of graphene sensors in a soft, stretchable porous mesh scaffold they developed that has close structural and mechanical properties to human tissue and which can be applied non-invasively to cardiac tissue.

“No one has ever done this before,” says Gao. “Graphene can survive in a biological environment without degrading for a very long time and not lose its conductivity, so we can monitor the CMT across its entire maturation process.”

“This is crucial for a number of reasons,” adds Yao. “Our sensor can give real-time feedback to scientists and drug researchers, and it can do so in a cost-effective way. We take pride in using the insights of electrical engineering to help build tools that can be useful to a wide range of researchers.”

In the future, Gao says, he hopes to be able to adapt his sensor to grander scales, even to in vivo monitoring, which would provide the best-possible data to help solve cardiac disease.

This research was supported by the Army Research Office, the National Institutes of Health, the U.S. National Science Foundation, the Semiconductor Research Corporation, and the Link Foundation, as well as the Institute for Applied Life Sciences at UMass Amherst.

Here’s a link to and a citation for the paper,

Graphene-integrated mesh electronics with converged multifunctionality for tracking multimodal excitation-contraction dynamics in cardiac microtissues by Hongyan Gao, Zhien Wang, Feiyu Yang, Xiaoyu Wang, Siqi Wang, Quan Zhang, Xiaomeng Liu, Yubing Sun, Jing Kong & Jun Yao. Nature Communications volume 15, Article number: 2321 (2024) DOI: https://doi.org/10.1038/s41467-024-46636-7 Published: 14 March 2024

This paper is open access.