Monthly Archives: September 2024

Moving past xenobots (living robots based on frog stem cells)

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Laura Tran’s June 14, 2024 article for The Scientist gives both a brief history of Michael Levin’s and his team’s work on developing living robots using stem cells from an African clawed frog (known as Xenopus laevis) and offers an update on the team’s work into synthetic lifeforms. First, the xenobots, Note 1: This could be difficult for people with issues regarding animal experimentation Note 1: Links have been removed,

Ibegan with little pieces of embryos scooting around in a dish. In 1998, these unassuming cells caught the attention of Michael Levin, then a postdoctoral researcher studying cell biology at Harvard University. He recalled simply recording a video before tucking the memory away. Nearly two decades later, Levin, now a developmental and synthetic biologist at Tufts University, experienced a sense of déjà vu. He observed that as a student transplanted tissues from one embryo to another, some loose cells swam free in the dish. 

Levin had a keen interest in the collective intelligence of cells, tissues, organs, and artificial constructs within regenerative medicine, and he wondered if he could explore the plasticity and harness the untapped capabilities of these swirling embryonic stem cells. “At that point, I started thinking that this is probably an amazing biorobotics platform,” recalled Levin. He rushed to describe this idea to Douglas Blackiston, a developmental and synthetic biologist at Tufts University who worked alongside Levin. 

At the time, Blackiston was conducting plasticity research to restore vision in blind African clawed frog tadpoles, Xenopus laevis, a model organism used to understand development. Blackiston transplanted the eyes to unusual places, such as the back of the head or even the tail, to test the integration of transplanted sensory organs.1 The eye axons extended to either the gut or spinal cord. In a display of dynamic plasticity, transplanted eyes on the tail that extended an optic nerve into the spinal cord restored the tadpoles’ vision.2 

…

In a similar vein, Josh Bongard, an evolutionary roboticist at the University of Vermont and Levin’s longtime colleague, pondered how robots could evolve like animals. He wanted to apply biological evolution to a machine by tinkering with the brains and bodies of robots and explored this idea with Sam Kriegman, then a graduate student in Bongard’s group and now an assistant professor at Northwestern University. Kriegman used evolutionary algorithms and artificial intelligence (AI) to simulate biological evolution in a virtual creature before teaming up with engineers to construct a physical version. 

…

i have two stories about the Xenobots. I was a little late to the party, so, the June 21, 2021 posting is about xenobots 2.0 and their ability to move and the June 8, 2022 posting is about their ability to reproduce.

Tran’s June 14, 2024 article provides the latest update, Note: Links have been removed,

Evolving Beyond the Xenobot

“People thought this was a one-off froggy-specific result, but this is a very profound thing,” emphasized Levin. To demonstrate its translatability in a non-frog model, he wondered, “What’s the furthest from an embryonic frog? Well, that would be an adult human.”

He enlisted the help of Gizem Gumuskaya, a synthetic biologist with an architectural background in Levin’s group, to tackle this challenge of creating biological robots using human cells to create anthrobots.8 While Gumuskaya was not involved with the development of xenobots, she drew inspiration from their design. By using adult human tracheal cells, she found that adult cells still displayed morphologic plasticity.

…

There are several key differences between xenobots and anthrobots: species, cell source (embryonic or adult), and the anthrobots’ ability to self-assemble without manipulation. “When considering applications, as a rule of thumb, xenobots are better suited to the environment. They exhibit higher durability, require less maintenance, and can coexist within the environment,” said Gumuskaya.

Meanwhile, there is greater potential for the use of mammalian-derived biobots in biomedical applications. This could include localized drug delivery, deposition into the arteries to break up plaque buildup, or deploying anthrobots into tissue to act as biosensors. “[Anthrobots] are poised as a personalized agent with the same DNA but new functionality,” remarked Gumuskaya.

…

Here’s a link to and a citation for the team’s latest paper,

Motile Living Biobots Self-Construct from Adult Human Somatic Progenitor Seed Cells by Gizem Gumuskaya, Pranjal Srivastava, Ben G. Cooper, Hannah Lesser, Ben Semegran, Simon Garnier, Michael Levin. Advanced Science Volume 11, Issue 4 January 26, 2024 2303575 DOI: https://doi.org/10.1002/advs.202303575 First published: 30 November 2023

This paper is open access.

Bio-hybrid robotics (living robots) needs public debate and regulation

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A July 23, 2024 University of Southampton (UK) press release (also on EurekAlert but published July 22, 2024) describes the emerging science/technology of bio-hybrid robotics and a recent study about the ethical issues raised, Note 1: bio-hybrid may also be written as biohybrid; Note 2: Links have been removed,

Development of ‘living robots’ needs regulation and public debate

Researchers are calling for regulation to guide the responsible and ethical development of bio-hybrid robotics – a ground-breaking science which fuses artificial components with living tissue and cells.

In a paper published in Proceedings of the National Academy of Sciences [PNAS] a multidisciplinary team from the University of Southampton and universities in the US and Spain set out the unique ethical issues this technology presents and the need for proper governance.

Combining living materials and organisms with synthetic robotic components might sound like something out of science fiction, but this emerging field is advancing rapidly. Bio-hybrid robots using living muscles can crawl, swim, grip, pump, and sense their surroundings. Sensors made from sensory cells or insect antennae have improved chemical sensing. Living neurons have even been used to control mobile robots.

Dr Rafael Mestre from the University of Southampton, who specialises in emergent technologies and is co-lead author of the paper, said: “The challenges in overseeing bio-hybrid robotics are not dissimilar to those encountered in the regulation of biomedical devices, stem cells and other disruptive technologies. But unlike purely mechanical or digital technologies, bio-hybrid robots blend biological and synthetic components in unprecedented ways. This presents unique possible benefits but also potential dangers.”

Research publications relating to bio-hybrid robotics have increased continuously over the last decade. But the authors found that of the more than 1,500 publications on the subject at the time, only five considered its ethical implications in depth.

The paper’s authors identified three areas where bio-hybrid robotics present unique ethical issues: Interactivity – how bio-robots interact with humans and the environment, Integrability – how and whether humans might assimilate bio-robots (such as bio-robotic organs or limbs), and Moral status.

In a series of thought experiments, they describe how a bio-robot for cleaning our oceans could disrupt the food chain, how a bio-hybrid robotic arm might exacerbate inequalities [emphasis mine], and how increasing sophisticated bio-hybrid assistants could raise questions about sentience and moral value.

“Bio-hybrid robots create unique ethical dilemmas,” says Aníbal M. Astobiza, an ethicist from the University of the Basque Country in Spain and co-lead author of the paper. “The living tissue used in their fabrication, potential for sentience, distinct environmental impact, unusual moral status, and capacity for biological evolution or adaptation create unique ethical dilemmas that extend beyond those of wholly artificial or biological technologies.”

The paper is the first from the Biohybrid Futures project led by Dr Rafael Mestre, in collaboration with the Rebooting Democracy project. Biohybrid Futures is setting out to develop a framework for the responsible research, application, and governance of bio-hybrid robotics.

The paper proposes several requirements for such a framework, including risk assessments, consideration of social implications, and increasing public awareness and understanding.

Dr Matt Ryan, a political scientist from the University of Southampton and a co-author on the paper, said: “If debates around embryonic stem cells, human cloning or artificial intelligence have taught us something, it is that humans rarely agree on the correct resolution of the moral dilemmas of emergent technologies.

“Compared to related technologies such as embryonic stem cells or artificial intelligence, bio-hybrid robotics has developed relatively unattended by the media, the public and policymakers, but it is no less significant. We want the public to be included in this conversation to ensure a democratic approach to the development and ethical evaluation of this technology.”

In addition to the need for a governance framework, the authors set out actions that the research community can take now to guide their research.

“Taking these steps should not be seen as prescriptive in any way, but as an opportunity to share responsibility, taking a heavy weight away from the researcher’s shoulders,” says Dr Victoria Webster-Wood, a biomechanical engineer from Carnegie Mellon University in the US and co-author on the paper.

“Research in bio-hybrid robotics has evolved in various directions. We need to align our efforts to fully unlock its potential.”

Here’s a link to and a citation for the paper,

Ethics and responsibility in biohybrid robotics research by Rafael Mestre, Aníbal M. Astobiza, Victoria A. Webster-Wood, Matt Ryan, and M. Taher A. Saif. PNAS 121 (31) e2310458121 July 23, 2024 DOI: https://doi.org/10.1073/pnas.2310458121

This paper is open access.

Cyborg or biohybrid robot?

Earlier, I highlighted “… how a bio-hybrid robotic arm might exacerbate inequalities …” because it suggests cyborgs, which are not mentioned in the press release or in the paper, This seems like an odd omission but, over the years, terminology does change although it’s not clear that’s the situation here.

I have two ‘definitions’, the first is from an October 21, 2019 article by Javier Yanes for OpenMind BBVA, Note: More about BBVA later,

…

The fusion between living organisms and artificial devices has become familiar to us through the concept of the cyborg (cybernetic organism). This approach consists of restoring or improving the capacities of the organic being, usually a human being, by means of technological devices. On the other hand, biohybrid robots are in some ways the opposite idea: using living tissues or cells to provide the machine with functions that would be difficult to achieve otherwise. The idea is that if soft robots seek to achieve this through synthetic materials, why not do so directly with living materials?

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In contrast, there’s this from “Biohybrid robots: recent progress, challenges, and perspectives,” Note 1: Full citation for paper follows excerpt; Note 2: Links have been removed,

…

2.3. Cyborgs

Another approach to building biohybrid robots is the artificial enhancement of animals or using an entire animal body as a scaffold to manipulate robotically. The locomotion of these augmented animals can then be externally controlled, spanning three modes of locomotion: walking/running, flying, and swimming. Notably, these capabilities have been demonstrated in jellyfish (figure 4(A)) [139, 140], clams (figure 4(B)) [141], turtles (figure 4(C)) [142, 143], and insects, including locusts (figure 4(D)) [27, 144], beetles (figure 4(E)) [28, 145–158], cockroaches (figure 4(F)) [159–165], and moths [166–170].

….

The advantages of using entire animals as cyborgs are multifold. For robotics, augmented animals possess inherent features that address some of the long-standing challenges within the field, including power consumption and damage tolerance, by taking advantage of animal metabolism [172], tissue healing, and other adaptive behaviors. In particular, biohybrid robotic jellyfish, composed of a self-contained microelectronic swim controller embedded into live Aurelia aurita moon jellyfish, consumed one to three orders of magnitude less power per mass than existing swimming robots [172], and cyborg insects can make use of the insect’s hemolymph directly as a fuel source [173].

…

So, sometimes there’s a distinction and sometimes there’s not. I take this to mean that the field is still emerging and that’s reflected in evolving terminology.

Here’s a link to and a citation for the paper,

Biohybrid robots: recent progress, challenges, and perspectives by Victoria A Webster-Wood, Maria Guix, Nicole W Xu, Bahareh Behkam, Hirotaka Sato, Deblina Sarkar, Samuel Sanchez, Masahiro Shimizu and Kevin Kit Parker. Bioinspiration & Biomimetics, Volume 18, Number 1 015001 DOI 10.1088/1748-3190/ac9c3b Published 8 November 2022 • © 2022 The Author(s). Published by IOP Publishing Ltd

This paper is open access.

A few notes about BBVA and other items

BBVA is Banco Bilbao Vizcaya Argentaria according to its Wikipedia entry, Note: Links have been removed,

Banco Bilbao Vizcaya Argentaria, S.A. (Spanish pronunciation: [ˈbaŋko βilˈβao βiθˈkaʝa aɾxenˈtaɾja]), better known by its initialism BBVA, is a Spanish multinational financial services company based in Madrid and Bilbao, Spain. It is one of the largest financial institutions in the world, and is present mainly in Spain, Portugal, Mexico, South America, Turkey, Italy and Romania.[2]

BBVA’s OpenMind is, from their About us page,

OpenMind: BBVA’s knowledge community

OpenMind is a non-profit project run by BBVA that aims to contribute to the generation and dissemination of knowledge about fundamental issues of our time, in an open and free way. The project is materialized in an online dissemination community.

“Sharing knowledge for a better future“.

At OpenMind we want to help people understand the main phenomena affecting our lives; the opportunities and challenges that we face in areas such as science, technology, humanities or economics. Analyzing the impact of scientific and technological advances on the future of the economy, society and our daily lives is the project’s main objective, which always starts on the premise that a broader and greater quality knowledge will help us to make better individual and collective decisions.

As for other items, you can find my latest (biorobotic, cyborg, or bionic depending what terminology you what to use) jellyfish story in this June 6, 2024 posting, the Biohybrid Futures project mentioned in the press release here, and also mentioned in the Rebooting Democracy project (unexpected in the context of an emerging science/technology) can be found here on this University of Southampton website.

Finally, you can find more on these stories (science/technology announcements and/or ethics research/issues) here by searching for ‘robots’ (tag and category), ‘cyborgs’ (tag), ‘machine/flesh’ (tag), ‘neuroprosthetic’ (tag), and human enhancement (category).

Enhance sunscreen without harming the environment by using octopus and squid pigments

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These days it seems experts are encouraging people wear sunscreen all year round. Anyway, that’s my excuse for claiming that this is a timely announcement, from a July 22, 2024 news item on phys.org,

When Northeastern [Northeastern University; Boston, Massachusetts] graduate Camille Martin and associate professor Leila Deravi co-founded Seaspire, a skincare ingredients company inspired by pigment in octopus and squid, their goal was to create a product that is good for your skin and the environment.

New research shows that they are on the right track.

…

A July 19, 2024 Northeastern University news release by Cynthia McCormick Hibbert, which originated the news item, reveals more about the research, Note: Links have been removed,

A paper published in the International Journal of Cosmetic Science says that Xanthochrome, a synthesized version of a molecule found in cephalopods such as squid, octopus and cuttlefish, boosts levels of sunscreen protection in combination with zinc oxide while having no adverse effects on coral cuttings.

The marine safety findings are important because “there’s a lot of toxicities involved with (traditional) UV filters in sunscreens,” says Deravi, who is Seaspire’s scientific adviser and an associate professor of chemistry and chemical biology.

“Some of the chemical UV-filters in particular are known to create reactive oxygen species that are not only bad for the environment but can also seep into our skin and cause systemic toxicities,” she says.

The result is a pressing need for environmentally friendly ingredients, says Martin, who got her Ph.D. in chemistry from Northeastern in 2019 and has served as Seaspire’s CEO since its founding that year.

“The industry is really excited about new materials innovations,” she says. “Everything we do as a biotechnology company is centered around leveraging marine animals as a source of inspiration for the next generation of skin care ingredients.”

From lab to market

The goal of Seaspire, Martin says, is to make Xanthochrome available to skin care product manufacturers and distributors up and down the supply chain so that it ends up in a wide range of ski care and personal care products including sunscreens, anti-aging applications and functional color cosmetics.

“We are just wrapping up the research and development on it now and actively looking for partnerships to bring this to market,” Deravi says.

Produced as a brown, textured powder, Xanthochrome has potent antioxidant and skin restorative properties as well as having light scattering qualities that provide protection against photoaging, Martin and Deravi say.

Martin says Xanthochrome is the trade name for a chemically synthesized version of xanthommatin, which is found in the skin of cuttlefish, octopus and squid and in insects as well.

“The secret to the cephalopods’ unique coloration is derived from its multifunctional chemical compounds, which we identified in our lab at Northeastern,” Deravi says.

“Camille’s Ph.D. work was the first to show that these small molecules inside cephalopod skin that contribute to camouflage in the animal also have really interesting antioxidant properties,” Deravi says.

“They’re free radical scavengers, which are very important for skin health and skin barrier function,” she says.

“And then they also have pretty important optical properties protecting against exposure to sunlight, which is the main function of some UV filters and sunscreens,” Deravi says.

“We didn’t create a new molecule,” Martin says. “We were able to isolate and characterize the properties of the biomolecules found within cephalopods, engineer a bio-identical version of the naturally occurring material and position Xanthochrome as a new active ingredient that provides a wide range of skin care benefits.”

“It’s a really interesting space where you have a single molecule that can have so many functions,” she says.

Previous research showed Xanthochrome, unlike the parabens that often go into sunscreens, is not an endocrine disruptor.

The most recent study shows that it boosts the ultraviolet protection of zinc oxide, which the U.S. Food and Drug Administration considers a safe and effective ingredient in sunscreen, by 28% and the blocking potential of visible light by 45%.

It also showed Xanthochrome did not have an adverse effect on coral cuttings even at concentrations five times higher than what are used in typical formulations.

Martin and Deravi hope that skincare product manufacturers see Xanthochrome as a next-generation ingredient on the heels of retinoids and vitamin C and hyaluronic acid.

“We’re creating products that can really be applied and adopted across a wide range of users,” Martin says. “We are creating something that is not only safe for all people, but also the environment.”

“You have to prove the new raw materials are safe for humans and also for the ocean, where ultimately every product is going to get washed into,” Deravi says.

Here’s a link to and a citation for the paper,

Using cephalopod-inspired chemistry to extend long-wavelength ultraviolet and visible light protection of mineral sunscreens by Leila F. Deravi, Isabel Cui, Camille A. Martin. International Journal of Cosmetic Science (2024) DOI: https://doi.org/10.1111/ics.12993 First published: 19 July 2024

This paper is behind a paywall.

The Seaspire Skincare website does not have any information about where you might access products with Xanthochrome. I’ll be keeping watch hoping to see some products in the not too distant future.

Painless, wearable patch for continuous smartphone monitoring of critical health data from Canadian researchers

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A June 18, 2024 McMaster University news release also on EurekAlert and on the University of Waterloo news website) by Wade Hemsworth describes the ‘Wearable Aptalyzer’, Note: A link has been removed,

Researchers at two Ontario universities have developed a pain-free, wearable sensor that can continuously monitor levels of blood sugar, lactates and other critical health indicators for weeks at a time, sending results to a smartphone or other device.

The Wearable Aptalyzer, created by a team featuring researchers from McMaster University and the University of Waterloo, uses an array of tiny hydrogel needles that penetrate just deeply enough to reach the interstitial fluid beneath the skin, but not far enough to reach the blood vessels or nerves.

The patch gathers and sends information about markers in the fluid to an electronic device such as a smart phone, creating an ongoing record of patterns in the rise and fall of critical biomarkers.

Once developed for clinical use, it will allow health professionals to access current medical information that today is available only retrospectively after blood tests and lab work.

The new technology could make monitoring the markers of specific diseases and conditions as simple as tracking pulse, blood pressure and other vital signs. The researchers describe the work in a new paper published today [version of record published May 16, 2024] in the journal Advanced Materials.

“This technology can provide real-time information about both chronic and acute health conditions, allowing caregivers to act more quickly and with greater certainty when they see trouble,” says one of the paper’s two corresponding authors, McMaster’s Leyla Soleymani,  professor of Engineering Physics who holds the Canada Research Chair in Miniaturized Biomedical Devices.

“The Wearable Aptalyzer is a general platform, meaning it can measure any biomarkers of interest, ranging from diabetes to cardiac biomarkers,” says corresponding author Mahla Poudineh, an assistant professor and director of the IDEATION Lab in the Department of Electrical and Computer Engineering at Waterloo. “Continuous health monitoring doesn’t just help catch diseases early and track how treatments are working. It also helps us understand how diseases happen, filling in important gaps in our knowledge that need attention.”

A user would apply and remove the patch much like a small bandage held in place with barely visible, soft hooks. The convenience is likely to appeal to diabetics and others who test themselves by drawing samples of blood or by using solid monitoring patches with metal needles that penetrate deeper and rely on less specific electrodes.

The greatest promise of the technology, though, may lie in its ability to produce weeks’ worth of meaningful results at a time, and to transmit data to electronic devices experts can read without sophisticated equipment.

Among the other potential applications, the Wearable Aptalyzer can make it possible to read and send data that signals cardiac events in real time, making it a potentially valuable tool for monitoring patients in ambulances and emergency rooms, and during treatment. The same technology can readily be adapted to monitor the progress and treatment of many chronic illnesses, including cancers, the researchers say.

The technology holds promise for improving care use in remote care settings, such as northern Indigenous communities set far from hospitals, or on space flights. Data from the Wearable Aptalyzer can signal trouble before symptoms become apparent, making it more likely patients can receive timely care.

The next steps in developing the technology for broad use include human trials and regulatory approvals. The researchers are seeking partners to help commercialize the technology.

The paper’s lead authors are Fatemeh Bakhshandeh of McMaster and Hanjia Zheng of Waterloo. Together with Soleymani and Poudineh, their co-authors are Waterloo’s Sadegh Sadeghzadeh, Irfani Ausri, Fatemeh Keyvani, Fasih Rahman, Joe Quadrilatero, and Juewen Liu, and McMaster’s Nicole Barra, Payel Sen, and Jonathan Schertzer.

Caption: The monitoring patch as compared to a 25-cent coin for scale. Credit: University of Waterloo

Here’s a link to and a citation for the paper,

Wearable Aptalyzer Integrates Microneedle and Electrochemical Sensing for In Vivo Monitoring of Glucose and Lactate in Live Animals by Fatemeh Bakhshandeh, Hanjia Zheng, Nicole G. Barra, Sadegh Sadeghzadeh, Irfani Ausri, Payel Sen, Fatemeh Keyvani, Fasih Rahman, Joe Quadrilatero, Juewen Liu, Jonathan D. Schertzer, Leyla Soleymani, Mahla Poudineh. Advanced Materials 2313743 DOI: https://doi.org/10.1002/adma.202313743 First online version of record published: 16 May 2024

This paper is open access.

Designers make dissolvable textiles from gelatin

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Am I the only one wondering what happens if your textiles start dissolving early? This excerpt from a June 17, 2024 news item on ScienceDaily announcing the research does not address my quandary,

Introducing the fashion of the future: A T-shirt that you can wear a few times, then, when you get bored with it, dissolve and recycle to make a new shirt.

Researchers at the ATLAS Institute at the University of Colorado Boulder are now one step closer to that goal. In a new study, the team of engineers and designers developed a DIY machine that spins textile fibers made of materials like sustainably sourced gelatin. The group’s “biofibers” feel a bit like flax fiber and dissolve in hot water in minutes to an hour.

…

The quandary is addressed in a manner of speaking in a June 17, 2024 University of Colorado at Boulder news release (also on EurekAlert) by Daniel Strain, which originated the news item, that also gives more context for the research and explains what the researchers are hoping to achieve, Note: A link has been removed,

“When you don’t want these textiles anymore, you can dissolve them and recycle the gelatin to make more fibers,” said Michael Rivera, a co-author of the new research and assistant professor in the ATLAS Institute and Department of Computer Science.

The study tackles a growing problem around the world: In 2018 alone, people in the United States added more than 11 million tons of textiles to landfills, according to the Environmental Protection Agency—nearly 8% of all municipal solid waste produced that year. 

The researchers envision a different path for fashion.

Their machine is small enough to fit on a desk and cost just $560 to build. Lázaro Vásquez [Eldy Lázaro Vásquez, doctoral student in the ATLAS Institute,] hopes the device will help designers around the world experiment with making their own biofibers.

“You could customize fibers with the strength and elasticity you want, the color you want,” she said. “With this kind of prototyping machine, anyone can make fibers. You don’t need the big machines that are only in university chemistry departments.”

Spinning threads

The study arrives as fashionistas, roboticists and more are embracing a trend known as “smart textiles.” Levi’s Trucker Jacket with Jacquard by Google, for example, looks like a denim coat but includes sensors that can connect to your smartphone. 

But such clothing of the future comes with a downside, Rivera said:

“That jacket isn’t really recyclable. It’s difficult to separate the denim from the copper yarns and the electronics.”

To imagine a new way of making clothes, the team started with gelatin. This springy protein is common in the bones and hooves of many animals, including pigs and cows. Every year, meat producers throw away large volumes of gelatin that doesn’t meet requirements for cosmetics or food products like Jell-O. (Lázaro Vásquez bought her own gelatin, which comes as a powder, from a local butcher shop).

She and her colleagues decided to turn that waste into wearable treasure.

The group’s machine uses a plastic syringe to heat up and squeeze out droplets of a liquid gelatin mixture. Two sets of rollers in the machine then tug on the gelatin, stretching it out into long, skinny fibers—not unlike a spider spinning a web from silk. In the process, the fibers also pass through liquid baths where the researchers can introduce bio-based dyes or other additives to the material. Adding a little bit of genipin, an extract from fruit, for example, makes the fibers stronger.

Dissolving duds

Lázaro Vásquez said designers may be able to do anything they can imagine with these sorts of textiles.

As a proof of concept, the researchers made small sensors out of gelatin fibers and cotton and conductive yarns, similar to the makeup of a Jacquard jacket. The team then submerged these patches in warm water. The gelatin dissolved, releasing the yarns for easy recycling and reuse.

Designers could tweak the chemistry of the fibers to make them a little more resilient, Lázaro Vásquez said—you wouldn’t want your jacket to disappear in the rain. [emphases mine] They could also play around with spinning similar fibers from other natural ingredients. Those materials include chitin, a component of crab shells, or agar-agar, which comes from algae.

“We’re trying to think about the whole lifecycle of our textiles,” Lázaro Vásquez said. “That begins with where the material is coming from. Can we get it from something that normally goes to waste?”

Here’s a link to and a citation for the paper,

Desktop Biofibers Spinning: An Open-Source Machine for Exploring Biobased Fibers and Their Application Towards Sustainable Smart Textile Design by Eldy S. Lazaro Vasquez, Mirela Alistar, Laura Devendorf, and Michael L. Rivera. CHI ’24: Proceedings of the CHI Conference on Human Factors in Computing Systems May 2024 Article No.: 856, Pages 1 – 18 DOI: https://doi.org/10.1145/3613904.3642387 Published: 11 May 2024

This paper is behind a paywall.

Let’s hope somebody (researcher or designer or ???) take a more extensive approach to solving the problem of fabrics that could dissolve prematurely.

Fashion, sustainability, and the protein threads that bind textiles and cosmetics

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I’m starting with a somewhat enthusiastic overview of the role synthetic biology is playing in the world of clothing and cosmetics in The Scientist and following it up with some stories about fish leather, no synthetic biology involved but all of these stories are about sustainability and fashion and, in one case, cosmetics.

Fashionable synthetic biology

Meenakshi Prabhune’s June 14, 2024 article in The Scientist, in addition to the overview, provides information that explains how some of the work on textiles and leather is being used in the production of cosmetics. She starts with a little history/mythology and then launches into the synthetic biology efforts to produce silk and leather suitable for consumer use, Note: Links have been removed,

Once upon a time, circa 2700 BC in China, empress Xi Ling Shi was enjoying her afternoon tea under a mulberry tree, when a silkworm cocoon fell from the tree into her tea. She noticed that on contact with the hot beverage, the cocoon unraveled into a long silky thread. This happy accident inspired her to acquire these threads in abundance and fashion them into an elegant fabric. 

So goes the legend, according to the writings of Confucius, about the discovery of silk and the development of sericulture in ancient China. Although archaeological evidence from Chinese ruins dates the presence of silk to 8500 years ago, hinting that the royal discovery story was spun just like the silk fabric, one part of the legend rings true.1 The Chinese royals played a pivotal role in popularizing silk as a symbol of status and wealth. By 130 BC, emperors in the Ancient Civilizations across the world desired to be clad in silken garments, paving the Silk Road that opened trade routes from China to the West. 

While silk maintained its high-society status over the next thousands of years, the demand for easy-to-use materials grew among mass consumers. In the early 20th century, textile developers applied their new-found technological prowess to make synthetic materials: petrochemical-based polymer blended textiles with improved durability, strength, and convenience. 

…

In their quest to make silk powerful again, not by status but rather by thread strength, scientists turned to an arachnoid. Dragline silk, the thread by which the spider hangs itself from the web, is one of the strongest fibers; its tensile strength—a measure of how much a polymer deforms when strained—is almost thrice that of silkworm silk.2 

Beyond durable fashion garments, tough silk fibers are coveted in parachutes, military protective gear, and automobile safety belts, among other applications, so scientists are keen to pull on these threads. While traditional silk production relies on sericulture, arachnophobes can relax: spider farms are not a thing.

“Spiders make very little silk and are quite territorial. So, the only way to do it is to make microbes that make the protein,” said David Breslauer, cofounder and chief technology officer at Bolt Threads, a bio apparel company. 

For decades, researchers have coaxed microbes into churning their metabolites in large fermentation tanks, which they have harvested to solve dire crises in many areas. For instance, when pharmaceuticals struggled to meet the growing demand for insulin through the traditional methods of extraction from animal pancreas, researchers at Genentech sought the aid of E. coli to generate recombinant insulin for mass production in 1978.3  [emphases mine]

…

Prabhune’s June 14, 2024 article notes some difficulties with spider silk, Note: Links have been removed,

… researchers soon realized that producing spider silk in microbes was no easy feat. The spider silk protein, spidroin, is larger than 300 kDa in size—a huge jump from the small 6 kDa recombinant insulin. Bulky proteins impose a heavy metabolic load on the microbes and their production yield tanks. Also, spidroin consists of repeating regions of glycine and alanine amino acids that impart strength and elasticity to the material, but the host microbes struggle with protein folding and overexpression of the corresponding tRNA molecules.4  

…

… researchers had gotten close, but they hadn’t been able to synthesize the full spidroin protein. Since the molecular weight of the silk protein correlates with the strength of the silk thread, Zhang [Fuzhong Zhang, a synthetic biologist at Washington University in St. Louis] was determined to produce the entire protein to mimic the silk’s natural properties.5

To achieve this goal without pushing the metabolic limits of the bacteria, Zhang and his team literally broke down the problem. In 2018, they devised a recombinant spidroin by constructing two protein halves with split inteins—peptides known to catalyze ligation between proteins while splicing out their own residues—tagged at their ends. They synthesized the halves in separate E. coli cultures, mixed the two cultures, and ligated the proteins to yielded a recombinant spidroin of 556 kDa—a size that was previously considered unobtainable.6 The resulting silk fiber made from these recombinant spidroins matched the mechanical properties of natural spider silk fiber.

…

While synthesizing the high molecular weight protein validated their technical prowess and strategy, Zhang knew that the yield with this approach was going to be unavoidably low. “It was not even enough to make a simple shirt,” he said.

…

Zhang and his team did solve the problem of getting a higher yield but that led to another problem, from Prabhune’s June 14, 2024 article,

…

Breslauer echoed the importance of this step. He recalled how scaling up was the biggest challenge when he and his cofounder Dan Widmaier, chief executive officer at Bolt Threads, first set up shop in 2009. The duo met during their graduate studies. Breslauer, a material science student at the University of California, Berkeley, was fascinated by spider silk and sought help for synthesizing the protein in microbes. Luckily, he met Widmaier, a synthetic biology graduate student who was optimizing systems to study complex proteins.

When their collaboration to produce recombinant spider silk proteins in yeast yielded promising results, the duo decided to challenge the status quo in the textile industry by commercially producing bio-silk apparel, and Bolt Threads was born. The market transition, however, was not as smooth as the threads they produced. 

“There was so little innovation in the textile space, and brands were really eager to talk about innovation. It felt like there was demand there. Turns out, the desire for storytelling outweighed the desire for actual innovation with those brands,” Breslauer said. “We didn’t realize how adverse [sic] people were going to be to the idea because it was so unfamiliar.”

…

Prabhune’s June 14, 2024 article also covers leather and cosmetics, Note: Links have been removed,

David Williamson, a chemist and the chief operations officer at Modern Meadow and his team wanted to separate themselves from the herd. In their quest for sustainable alternatives, they went back to the basic biology and chemistry of the material. As leather is made from animal skin, it is rich in collagen, a structural protein abundant in the extracellular matrix of connective tissues. If the team could produce this primary component protein at scale, they would be able to process it into leather downstream. 

In about 2017, Williamson and his team developed a fermentation-based approach to produce collagen from yeast. While they achieved scalable production, there was one small hiccup. The protein properties of collagen alone did not yield the mechanical properties they needed for their leather-like material. 

…

The team went to the drawing board and analyzed the amino acid residues that contributed to collagen’s characteristics to look for a substitute protein. They found an alternative that had the desirable functional elements of collagen but was also sustainable and cost effective for industrial scale up: soy protein isolate. While tinkering with their recipes, they found the perfect combination for material strength by mixing in a bio-based polyurethane polymer with the protein to yield a refined bioalloy called Bio-VERA. 

…

As natural textiles are derived from animal skin, hair, or proteins, it is no surprise that many synthetic biologists in the textile space have also found a niche in cosmetics. Even as the Modern Meadow team transitioned away from their protein fermentation strategies to innovate Bio-VERA, they realized that they could still apply their expertise in skincare. While leathery is not an adjective one desires to associate with skin, collagen is an integral component in both. “When our bodies make collagen and build our extracellular matrices, one of the first proteins that they deposit is type three collagen. So, you can think of type three collagen almost like the structure or scaffold of a building,” explained Williamson.

…

To cater to the increasing demand for solutions to achieve younger looking skin, Williamson and his team engineered a recombinant collagen type three protein containing part of the protein sequence that is rich in binding domains for fibroblast interactions.9,10  “After you expose the extracellular matrix to this protein, it stimulates the fibroblasts to make more type three collagen. That type three collagen lays down type one collagen and elastin and fibronectin in a way that actually helps to turn back time, so to speak, to increase the ratio of type three collagen relative to type one collagen,” Williamson said. 

The Modern Meadow team are not the only ones to weave their textile strands into cosmetic applications. When Artur Cavaco-Paulo, a biological engineer at the University of Minho [Portugal], was studying wool fibers, he was struck by their structural similarities to human hair. “We decided that it would be a really good idea to transfer some of the knowledge that we had in wool textiles to human hair,” said Cavaco-Paulo. Particularly, he was interested in investigating solutions to fix hair strands damaged by highly alkaline chemical products. 

…

Over the next few years, Cavaco-Paulo developed […] shortlisted peptides into the K18 peptide product, which is now part of a commercially available leave-in conditioner. Cavaco-Paulo serves as the chief scientific officer at the biotech company K18. 

Although he started his career with textile research, Cavaco-Paulo favors the cosmetics sector with regards to returns on research and technology investment. “The personal care market is much more accustomed to innovation and has a much better and more fluid pipeline on innovation,” seconded Breslauer. “Whereas, [in] apparel, you really have to twist arms to get people to work with your material.” Bolt Threads ventured into the personal care space when Breslauer and his team serendipitously stumbled upon an alternative use for one of their textile proteins. 

…

While it’s not mentioned in Prabhune’s June 14, 2024 article, sustainability is mentioned on two of the company websites,

Bolt Threads

Bolt Threads is a material solutions company. With nature as our inspiration, we invent cutting-edge materials for the fashion and beauty industries to put us on a path toward a more sustainable future.

Through innovative collaborations with world-class brands and supply chain partners, we are on a mission to create way better materials for a way better world. Join us.

…

Modern Meadow

Modern Meadow is a climate-tech pioneer creating the future of materials through innovations in biology and material science.

​Our bio-materials technology platform with nature-inspired protein solutions delivers better performance, sustainability, scalability, and cost while reducing reliance on petrochemical and animal-based inputs.​

…

K18 has not adopted a ‘sustainability’ approach to marketing its hair care products.

Sustainability without synthetic biology: fish leather

In a January 3, 2022 posting I featured fish leather/skin in a story about the “Futures exhibition/festival” held at the Smithsonian Institute from November 20, 2021 to July 6, 2022.

Before getting to Futures, here’s a brief excerpt from a June 11, 2021 Smithsonian Magazine exhibition preview article by Gia Yetikyel about one of the contributors, Elisa Palomino-Perez (Note: A link has been removed),

Elisa Palomino-Perez sheepishly admits to believing she was a mermaid as a child. Growing up in Cuenca, Spain in the 1970s and ‘80s, she practiced synchronized swimming and was deeply fascinated with fish. Now, the designer’s love for shiny fish scales and majestic oceans has evolved into an empowering mission, to challenge today’s fashion industry to be more sustainable, by using fish skin as a material.

Luxury fashion is no stranger to the artist, who has worked with designers like Christian Dior, John Galliano and Moschino in her 30-year career. For five seasons in the early 2000s, Palomino-Perez had her own fashion brand, inspired by Asian culture and full of color and embroidery. It was while heading a studio for Galliano in 2002 that she first encountered fish leather: a material made when the skin of tuna, cod, carp, catfish, salmon, sturgeon, tilapia or pirarucu gets stretched, dried and tanned.

…

The history of using fish leather in fashion is a bit murky. The material does not preserve well in the archeological record, and it’s been often overlooked as a “poor person’s” material due to the abundance of fish as a resource. But Indigenous groups living on coasts and rivers from Alaska to Scandinavia to Asia have used fish leather for centuries. Icelandic fishing traditions can even be traced back to the ninth century. While assimilation policies, like banning native fishing rights, forced Indigenous groups to change their lifestyle, the use of fish skin is seeing a resurgence. Its rise in popularity in the world of sustainable fashion has led to an overdue reclamation of tradition for Indigenous peoples.

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Brendan Jones provides an update of sorts in his Alaska-forward take in his February 22, 2024 article “Fish Leather Is Incredibly Strong and Beautiful. Can Makers ‘Scale Up’? Meet artisans in Alaska and BC who are sustaining, and advancing, an ancient art.” for The Tyee,

Fish leather artist June Pardue began her journey into the craft not knowing where to start. Which was a problem, considering that she had been given the job of demonstrating for tourists how to tan fish skin at the Alaska Native Heritage Center in Anchorage. “I couldn’t find anyone to teach me,” Pardue said with a laugh.

“One day a guy from Mississippi noticed me fumbling around. He kindly waited until everyone had left. Then he said, ‘Do you want me to share my grandpappy’s recipe for tanning snake skins?’”

His cocktail of alcohol and glycerin allowed her to soften the skins — as tourists looked on — for future use in clothing and bags. This worked fine until she began to grow uncomfortable dumping toxins down the drain. Now she uses plant-based tannins like those found in willow branches after the season’s first snowmelt. She harvests the branches gingerly, allowing the trees to survive for the next generation of fish tanners.

Pardue, who teaches at the University of Alaska, was born on Kodiak Island, off the southern coast of the state, in Old Harbor village. Alutiiq and Iñupiaq, she was raised in Akhiok, population about 50, and Old Harbor.

Following her bumpy start at the heritage center, Pardue has since gone on to become one of Alaska’s and Canada’s most celebrated instructors and practitioners in the field of fish leather, lighting the way for others in Alaska and Canada.

Among the people Pardue has advised is CEO and founder of 7 Leagues tannery Tasha Nathanson, who is based in Vancouver. She met with Pardue to share her idea of creating a business built on making fish leather into boots and other items for a large customer base.

…

Before making her move to open a business, Nathanson spent a year running the numbers, she said. In 2022, the global fish leather market was valued at US$36.22 million. As fish tanneries open their doors and fashion houses take notice, the number is expected to grow 16 per cent annually, topping $100 million by 2030.

“Salmon certainly don’t come to mind when you think of tanning, but people are catching on,” said Judith Lehmann, a Sitka-based expert in fish leather, who took Pardue’s class. (The Tyee reached Lehmann in Panama, where she was experimenting with skins of bonito and mahi mahi.)

Growing numbers of buyers are willing to pay for not only the beauty but also the remarkable durability fish leather can offer. California-based eco-fashion designer Hailey Harmon’s company Aitch Aitch sells the Amelia, a teal backpack made of panelled salmon leather, for $795.

One company in France has started to collect fish skins from restaurants — material that would otherwise end up in trash cans — to make luxury watch bands and accessories. Designers like Prada, Louis Vuitton and Christian Dior have incorporated fish leather into their lines. Even Nike introduced running shoes made of perch skin.

Whether they know it or not, today’s trendsetters are rooted in ancient history. “People have been working with fish skins for thousands of years,” Pardue said. “Ireland, Iceland, Norway, China, Japan — it’s an age-old practice.”

…

“On a molecular level, fibres in fish leather are cross-hatched, as opposed to cow leather, which is just parallel,” Nathanson explained. “So, pound for pound, this leather is stronger, which is great for shoes. And it’s more available, and eco-conscious. It’s a win across the board.”

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Jones’s February 22, 2024 article has some wonderful embedded pictures and Beth Timmins’s May 1, 2019 article for the BBC (British Broadcasting Corporation), while a little dated, offers more information about the international scene.

Synthetic biology is a scientific practice that I find disconcerting at times. That said, I’m glad to see more work on sustainable products however they are derived. On that note I have a couple of recent stories:

  • “Three century long development of a scientific idea: body armor made from silk” is the title of my July 11, 2024 posting
  • “Grown from bacteria: plastic-free vegan leather that dyes itself” is the title of my June 26, 2024 posting

Enjoy!

Better (safer, cheaper) battery invented for wearable tech

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A June 5, 2024 news item on phys.org announces new research into ‘aqueous’ wearable batteries,

Researchers have developed a safer, cheaper, better performing and more flexible battery option for wearable devices. A paper describing the “recipe” for their new battery type was published in the journal Nano Research Energy on June 3 [2024].

Fitness trackers. Smart watches. Virtual-reality headsets. Even smart clothing and implants. Wearable smart devices are everywhere these days. But for greater comfort, reliability and longevity, these devices will require greater levels of flexibility and miniaturization of their energy storage mechanisms, which are often frustratingly bulky, heavy and fragile. On top of this, any improvements cannot come at the expense of safety.

As a result, in recent years, a great deal of battery research has focused on the development of “micro” flexible energy storage devices, or MFESDs. A range of different structures and electrochemical foundations have been explored, and among them, aqueous micro batteries offer many distinct advantages.

…

A June 5, 2024 Tsinghua University press release on EurekAlert, which originated the news item, provides more detail,

Aqueous batteries—those that use a water-based solution as an electrolyte (the medium that allows transport of ions in the battery and thus creating an electric circuit) are nothing new. They have been around since the late 19th century. However, their energy density—or the amount of energy contained in the battery per unit of volume—is too low for use in things like electric vehicles as they would take up too much space. Lithium-ion batteries are far more appropriate for such uses.

At the same time, aqueous batteries are much less flammable, and thus safer, than lithium-ion batteries. They are also much cheaper. As a result of this more robust safety and low cost, aqueous options have increasingly been explored as one of the better options for MFESDs. These are termed aqueous micro batteries, or just AMBs.

“Up till now, sadly, AMBs have not lived up to their potential,” said Ke Niu, a materials scientist with the Guangxi Key Laboratory of Optical and Electronic Materials and Devices at the Guilin University of Technology—one of the lead researchers on the team. “To be able to be used in a wearable device, they need to withstand a certain degree of real-world bending and twisting. But most of those explored so far fail in the face of such stress.”

To overcome this, any fractures or failure points in an AMB would need to be self-healing following such stress. Unfortunately, the self-healing AMBs that have been developed so far have tended to depend on metallic compounds as the carriers of charge in the battery’s electric circuit. This has the undesirable side-effect of strong reaction between the metal’s ions and the materials that the electrodes (the battery’s positive and negative electrical conductors) are made out of. This in turn reduces the battery’s reaction rate (the speed at which the electrochemical reactions at the heart of any battery take place), drastically limiting performance.

“So we started investigating the possibility of non-metallic charge carriers, as these would not suffer from the same difficulties from interaction with the electrodes,” added Junjie Shi, another leading member of the team and a researcher with the School of Physics and Center zfor Nanoscale Characterization & Devices (CNCD) at the Huazhong University of Science and Technology in Wuhan.

The research team alighted upon ammonium ions, derived from abundantly available ammonium salts, as the optimal charge carriers. They are far less corrosive than other options and have a wide electrochemical stability window.

“But ammonium ions are not the only ingredient in the recipe needed to make our batteries self-healing,” said Long Zhang, the third leading member of the research team, also at CNCD.

For that, the team incorporated the ammonium salts into a hydrogel—a polymer material that can absorb and retain a large amount of water without disturbing its structure. This gives hydrogels impressive flexibility—delivering precisely the sort of self-healing character needed. Gelatin is probably the most well-known hydrogel, although the researchers in this case opted for a polyvinyl alcohol hydrogel (PVA) for its great strength and low cost.

To optimize compatibility with the ammonium electrolyte, titanium carbide—a ‘2D’ nanomaterial with only a single layer of atoms—was chosen for the anode (the negative electrode) material for its excellent conductivity. Meanwhile manganese dioxide, already commonly used in dry cell batteries, was woven into a carbon nanotube matrix (again to improve conductivity) for the cathode (the positive electrode).

Testing of the prototype self-healing battery showed it exhibited excellent energy density, power density, cycle life, flexibility, and self-healing even after ten self-healing cycles.

The team now aims to further develop and optimise their prototype in preparation for commercial production.

About Nano Research Energy

Nano Research Energy is launched by Tsinghua University Press and exclusively available via SciOpen, aiming at being an international, open-access and interdisciplinary journal. We will publish research on cutting-edge advanced nanomaterials and nanotechnology for energy. It is dedicated to exploring various aspects of energy-related research that utilizes nanomaterials and nanotechnology, including but not limited to energy generation, conversion, storage, conservation, clean energy, etc. Nano Research Energy will publish four types of manuscripts, that is, Communications, Research Articles, Reviews, and Perspectives in an open-access form.

About SciOpen

SciOpen is a professional open access resource for discovery of scientific and technical content published by the Tsinghua University Press and its publishing partners, providing the scholarly publishing community with innovative technology and market-leading capabilities. SciOpen provides end-to-end services across manuscript submission, peer review, content hosting, analytics, and identity management and expert advice to ensure each journal’s development by offering a range of options across all functions as Journal Layout, Production Services, Editorial Services, Marketing and Promotions, Online Functionality, etc. By digitalizing the publishing process, SciOpen widens the reach, deepens the impact, and accelerates the exchange of ideas.

Here’s a link to and a citation for the paper,

A self-healing aqueous ammonium-ion micro batteries based on PVA-NH4Cl hydrogel electrolyte and MXene-integrated perylene anode by Ke Niu, Junjie Shi, Long Zhang, Yang Yue, Mengjie Wang, Qixiang Zhang, Yanan Ma, Shuyi Mo, Shaofei Li, Wenbiao Li, Li Wen, Yixin Hou, Fei Long, Yihua Gao. Nano Research Energy (2024)DOI: https://doi.org/10.26599/NRE.2024.9120127 Published: 03 June 2024

This paper is open access by means of a “Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.”

Back to school: Stanford University (California) brings nanoscience to teachers and Ingenium brings STEAM to school

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I have two stories that fit into the ‘back to school’ theme, one from Stanford University and one from Ingenium (Canada’s Museums of Science and Innovation).

Stanford, nanoscience, and middle school teachers

h/t to Google Alert of August 27, 2024 (received via email) for information about a Stanford University programme for middle school teachers. From an August 27, 2024 article in the Stanford Report, Note: Links have been removed,

Crafting holographic chocolate, printing with the power of the sun, and seeing behind the scenes of cutting-edge research at the scale of one-billionth of a meter, educators participating in the Nanoscience Summer Institute for Middle School Teachers (NanoSIMST) got to play the role of students, for a change.

Teachers hailed from the Bay Area and Southern California – one had even come all the way from Arkansas – for the professional development program. NanoSIMST, run by nano@stanford, is designed to connect middle school teachers with activities, skills, and knowledge about science at the scale of molecules and atoms so they can incorporate it into their curriculum. NanoSIMST also prioritizes teachers from Title I schools, which are low-income schools with low-income student populations that receive federal funding to improve academic achievement.

…

Debbie Senesky, the site investigator and principal researcher on the nano@stanford project, highlighted the importance of nanoscience at the university. “It’s not just about focusing on research – we also have bigger impacts on entrepreneurs, start-ups, community colleges, and other educators who can use these facilities,” said Senesky, who is also an associate professor of aeronautics and astronautics and of electrical engineering. “We’re helping to train the next generation of people who can be a workforce in the nanotechnology and semiconductor industry.”

The program also supports education and outreach, including through NanoSIMST, which uniquely reaches out to middle school teachers due to the STEM education outcomes that occur at that age. According to a 2009 report by the Lemelson-MIT InvenTeam Initiative, even among teens who were interested in and felt academically prepared in their STEM studies, “nearly two-thirds of teens indicated that they may be discouraged from pursuing a career in science, technology, engineering or mathematics because they do not know anyone who works in these fields (31%) or understand what people in these fields do (28%).”

A teacher from the Oakland Unified School District, Thuon Chen, connected several other teachers from OUSD to attend NanoSIMST as a first-time group. He emphasized that young kids, especially in middle school, have a unique way of approaching new technologies. “Kids have this sense where they’re always pushing things and coming up with completely new uses, so introducing them to a new technology can give them a lot to work with.”

Over the course of four days in the summer, NanoSIMST provides teachers with an understanding of extremely small science and technology: they go through tours of the nano facilities, speak with scientists, perform experiments that can be conducted in the classroom, and learn about careers in nanotechnology and the semiconductor industry.

Tara Hodge, the teacher who flew all the way from Arkansas, was thrilled about bringing what she learned back with her. “I’m not a good virtual learner, honestly. That’s why I came here. And I’m really excited to learn about different hands-on activities. Anything I can get excited about, I know I can get my students excited about.”

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They have provided a video,

One comment regarding the host, Daniella Duran, the director of education and outreach for nano@stanford, she comments about nano being everywhere and, then, says “… everything has a microchip in it.” I wish she’d been a little more careful with the wording. Granted those microchips likely have nanoscale structures.

Ingenium’s STEAM (science, technology, engineering, arts, and mathematics) programmes for teachers across Canada

An August 27, 2024 Ingenium newsletter (received via email) lists STEAM resources being made available for teachers across the country.

There appears to be a temporary copy of the August 27, 2024 Ingenium newsletter here,

STEAM lessons made simple!

Another school year is about to begin, and whether you’re an experienced teacher or leading your first class, Ingenium has what you need to make your STEAM (science, technology, engineering, arts and math) lessons fun! With three museums of science and innovation – the Canada Agriculture and Food Museum, the Canada Aviation and Space Museum and the Canada Science and Technology Museum – under one umbrella, we are uniquely positioned to help your STEAM lessons come to life.

Embark on an exciting adventure with our bilingual virtual field trips and meet the animals in our barns, explore aviation technology, and conduct amazing science experiments.

Or take advantage of our FREE lesson plans, activities and resources to simplify and animate your classroom, all available in English and French. With Ingenium, innovation is at your fingertips!

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Bring the museum to your classroom with a virtual field trip!

Can’t visit in person? Don’t worry, Ingenium will bring the museum to you! All of our virtual field trips are led by engaging guides who will animate each subject with an entertaining and educational approach. Choose from an array of bilingual programs designed for all learners that cover the spectrum of STEAM subjects, including the importance of healthy soil, the genetic considerations of a dairy farm operation, the science of flight, simple machines, climate change and the various states of matter. There is so much to discover with Ingenium. Book your virtual field trip today!

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Here’s a video introduction to Ingenium’s offerings,

To get a look at all the resources, check out this temporary copy of the August 27, 2024 Ingenium newsletter here.

Systemic gene silencing in crops with engineered nanocomplexes

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Ultimately, the researchers are working on ways to make agriculture more sustainable but, in the meantime, there’s this June 7, 2024 news item on ScienceDaily describing this work,

Gene silencing in plants has faced significant challenges, primarily due to the difficulty of transporting RNA molecules across plant cell membranes and achieving systemic effects. Traditional genetic engineering methods are time-consuming and often limited by plant genotype. Due to these challenges, there is a pressing need for innovative solutions to facilitate efficient gene silencing and enhance crop productivity.

…

A June 7, 2024 news release, from Nanjing Agricultural University The Academy of Science (publisher of Horticulture Research), on EurekAlert, which originated the news item, goes on to describe the challenges and the proposed solution, Note: Links have been removed,

Gene silencing in plants has faced significant challenges, primarily due to the difficulty of transporting RNA molecules across plant cell membranes and achieving systemic effects. Traditional genetic engineering methods are time-consuming and often limited by plant genotype. Due to these challenges, there is a pressing need for innovative solutions to facilitate efficient gene silencing and enhance crop productivity.

Researchers from the University of Connecticut and Oak Ridge National Laboratory have developed an innovative method using cationized bovine serum albumin (cBSA) and double-stranded RNA (dsRNA) nanocomplexes to achieve effective systemic gene silencing in plants. Published (DOI: 10.1093/hr/uhae045) in Horticulture Research on February 22, 2024, this study demonstrates the potential of these nanocomplexes to overcome the limitations of traditional RNA delivery methods, offering a new tool for plant biotechnology.

The study presents the development of cBSA/dsRNA nanocomplexes for systemic gene silencing in tobacco and poplar plants. By modifying bovine serum albumin to carry a positive charge, researchers created nanocomplexes that bind dsRNA molecules, facilitating their transport and systemic gene silencing. Experiments demonstrated successful silencing of the DR5-GUS and 35S-GUS genes, achieving significant reductions in gene expression. This technology proved effective in delivering RNA molecules across plant cell membranes, overcoming the negative charge barrier of naked RNA applications. Offering a convenient, fast, and non-transgenic approach, this method holds promise for gene function characterization, crop improvement, and large-scale agricultural applications due to its scalability and cost-effectiveness.

Dr. Yi Li, a lead researcher on the project, stated, “The development of cBSA/dsRNA nanocomplexes represents a significant advancement in plant biotechnology. This technology not only facilitates efficient gene silencing but also offers a practical and scalable solution for improving crop productivity. We believe this method will pave the way for new applications in gene editing and agricultural research.”

The implications of this research are vast, offering a potential solution for transient gene silencing in field-grown crops, including orchard trees. This technology could enhance crop productivity by targeting genes that influence drought tolerance, fruit development, and stress resistance, all without the need for genetic modification. The scalable and inexpensive nature of this method could make it a game-changer for sustainable agriculture.

The research and the journal where it is published both have interesting pedigrees. From the June 7, 2024 news release,

Funding information

This work was supported by the USDA National Institute of Food and Agriculture SCRI (grant no. 2015-70016-23027) and the Connecticut-Storrs Agriculture Experimental Station.

About Horticulture Research

Horticulture Research is an open access journal of Nanjing Agricultural University and ranked number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. The journal is committed to publishing original research articles, reviews, perspectives, comments, correspondence articles and letters to the editor related to all major horticultural plants and disciplines, including biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.

You can add the UK to the US/China mix since the website hosting Horticulture Research is Oxford Academic,

Oxford Academic is Oxford University Press’s academic research platform, providing access to over 50,000 books and 500 journals

…

Finally, here’s a link to and a citation for the paper,

Engineered dsRNA–protein nanoparticles for effective systemic gene silencing in plants by Huayu Sun, Ankarao Kalluri, Dan Tang, Jingwen Ding, Longmei Zhai, Xianbin Gu, Yanjun Li, Huseyin Yer, Xiaohan Yang, Gerald A Tuskan, Zhanao Deng, Frederick G Gmitter Jr, Hui Duan, Challa Kumar, Yi Li. Horticulture Research, Volume 11, Issue 4, April 2024, uhae045, DOI: https://doi.org/10.1093/hr/uhae045
Published online: 22 February 2024

This paper is open access.