What a gorgeous tomato plant! Here’s more about the work that went into this plant and others in an April 29, 2025 news item on ScienceDaily,

When farmers apply pesticides to their crops, 30 to 50 percent of the chemicals end up in the air or soil instead of on the plants. Now, a team of researchers from MIT [Massachusetts Institute of Technology] and Singapore has developed a much more precise way to deliver substances to plants: tiny needles made of silk.

In a study published today in Nature Nanotechnology, the researchers developed a way to produce large amounts of these hollow silk microneedles. They used them to inject agrochemicals and nutrients into plants, and to monitor their health.

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An April 29, 2025 Massachusetts Institute of Technology (MIT) news release (also on EurekAlert) by Zach Winn, which originated the news item, delves further into the research, Note: Links have been removed,

“There’s a big need to make agriculture more efficient,” says Benedetto Marelli, the study’s senior author and an associate professor of civil and environmental engineering at MIT. “Agrochemicals are important for supporting our food system, but they’re also expensive and bring environmental side effects, so there’s a big need to deliver them precisely.”

Yunteng Cao PhD ’22, currently a postdoc [at?] Yale University, and Doyoon Kim, a former postdoc in the Marelli lab, led the study, which included a collaboration with the Disruptive and Sustainable Technologies for Agricultural Precision (DiSTAP) interdisciplinary research group at the Singapore-MIT Alliance for Research and Technology (SMART). 

In demonstrations, the team used the technique to give plants iron to treat a disease known as chlorosis, and to add vitamin B12 to tomato plants to make them more nutritious. The researchers also showed the microneedles could be used to monitor the quality of fluids flowing into plants and to detect when the surrounding soil contained heavy metals.

Overall, the researchers believe the microneedles could serve as a new kind of plant interface for real-time health monitoring and biofortification.

“These microneedles could be a tool for plant scientists so they can understand more about plant health and how they grow,” Marelli says. “But they can also be used to add value to crops, making them more resilient and possibly even increasing yields.”

The inner workings of plants

Accessing the inner tissues of living plants requires scientists to get through the plants’ waxy skin without causing too much stress. In previous work, the researchers used silk-based microneedles to deliver agrochemicals to plants in lab environments and to detect pH changes in living plants. But these initial efforts involved small payloads, limiting their applications in commercial agriculture.

“Microneedles were originally developed for the delivery of vaccines or other drugs in humans,” Marelli explains. “Now we’ve adapted it so that the technology can work with plants, but initially we could not deliver sufficient doses of agrochemicals and nutrients to mitigate stressors or enhance crop nutritional values.”

Hollow structures could increase the amount of chemicals microneedles can deliver, but Marelli says creating those structures at scale has historically required clean rooms and expensive facilities like the ones found inside the MIT.nano building.

For this study, Cao and Kim created a new way to manufacture hollow silk microneedles by combining silk fibroin protein with a salty solution inside tiny, cone-shaped molds. As water evaporated from the solution, the silk solidified into the mold while the salt forms crystalline structures inside the molds. When the salt was removed, it left behind in each needle a hollow structure or tiny pores, depending on the salt concentration and the separation of the organic and inorganic phases.

“It’s a pretty simple fabrication process. It can be done outside of a clean room — you could do it in your kitchen if you wanted,” Kim says. “It doesn’t require any expensive machinery.”

The researchers then tested their microneedles’ ability to deliver iron to iron-deficient tomato plants, which can cause a disease known as chlorosis. Chlorosis can decrease yields, but treating it by spraying crops is inefficient and can have environmental side effects. The researchers showed that their hollow microneedles could be used for the sustained delivery of iron without harming the plants.

The researchers also showed their microneedles could be used to fortify crops while they grow. Historically, crop fortification efforts have focused on minerals like zinc or iron, with vitamins only added after the food is harvested.

In each case, the researchers applied the microneedles to the stalks of plants by hand, but Marelli envisions equipping autonomous vehicles and other equipment already used in farms to automate and scale the process.

As part of the study, the researchers used microneedles to deliver vitamin B12, which is primarily found naturally in animal products, into the stalks of growing tomatoes, showing that vitamin B12 moved into the tomato fruits before harvest. The researchers propose their method could be used to fortify more plants with the vitamin.

Co-author Daisuke Urano, a plant scientist with DiSTAP, explains that “through a comprehensive assessment, we showed minimal adverse effects from microneedle injections in plants, with no observed short- or long-term negative impacts.”

“This new delivery mechanism opens up a lot of potential applications, so we wanted to do something nobody had done before,” Marelli explains.

Finally, the researchers explored the use of their microneedles to monitor the health of plants by studying tomatoes growing in hydroponic solutions contaminated with cadmium, a toxic metal commonly found in farms close to industrial and mining sites. They showed their microneedles absorbed the toxin within 15 minutes of being injected into the tomato stalks, offering a path to rapid detection.

Current advanced techniques for monitoring plant health, such as colorimetric and hyperspectral lead analyses, can only detect problems after plants growth is already being stunted. Other methods, such as sap sampling, can be too time-consuming.

Microneedles, in contrast, could be used to more easily collect sap for ongoing chemical analysis. For instance, the researchers showed they could monitor cadmium levels in tomatoes over the course of 18 hours.

A new platform for farming

The researchers believe the microneedles could be used to complement existing agricultural practices like spraying. The researchers also note the technology has applications beyond agriculture, such as in biomedical engineering.

“This new polymeric microneedle fabrication technique may also benefit research in microneedle-mediated transdermal and intradermal drug delivery and health monitoring,” Cao says.

For now, though, Marelli believes the microneedles offer a path to more precise, sustainable agriculture practices.

“We want to maximize the growth of plants without negatively affecting the health of the farm or the biodiversity of surrounding ecosystems,” Marelli says. “There shouldn’t be a trade-off between the agriculture industry and the environment. They should work together.”

This work was supported, in part, by the U.S. Office of Naval Research, the U.S. National Science Foundation, SMART, the National Research Foundation of Singapore, and the Singapore Prime Minister’s Office.

Here’s a link to and a citation for the paper,

Nanofabrication of silk microneedles for high-throughput micronutrient delivery and continuous sap monitoring in plants by Yunteng Cao, Doyoon Kim, Sally Shuxian Koh, Zheng Li, Federica Rigoldi, Julia Eva Fortmueller, Kasey Goh, Yilin Zhang, Eugene J. Lim, Hui Sun, Elise Uyehara, Raju Cheerlavancha, Yangyang Han, Rajeev J. Ram, Daisuke Urano & Benedetto Marelli. Nature Nanotechnology (2025) DOI: https://doi.org/10.1038/s41565-025-01923-2 Published: 29 April 2025

This paper is behind a paywall.