Tag Archives: salmon

AI for salmon recovery

Hopefully you won’t be subjected to a commercial prior to this 3 mins. 49 secs. video about the salmon and how artificial intelligence (AI) could make a difference in theirs and our continued survival,

Video caption: Wild Salmon Center is partnering with First Nations to pilot the Salmon Vision technology. (Credit: Olivia Leigh Nowak/Le Colibri Studio.)

An October 19, 2023 news item on phys.org announces this research, Note: Links have been removed,

Scientists and natural resource managers from Canadian First Nations, governments, academic institutions, and conservation organizations published the first results of a unique salmon population monitoring tool in Frontiers in Marine Science.

This groundbreaking new technology, dubbed “Salmon Vision,” combines artificial intelligence with age-old fishing weir technology. Early assessments show it to be remarkably adept at identifying and counting fish species, potentially enabling real-time salmon population monitoring for fisheries managers.

An October 19, 2023 Wild Salmon Center news release on EurekAlert, which originated the news item, provides more detail about the work,

“In recent years, we’ve seen the promise of underwater video technology to help us literally see salmon return to rivers,” says lead author Dr. Will Atlas, Senior Watershed Scientist with the Portland-based Wild Salmon Center. “That dovetails with what many of our First Nations partners are telling us: that we need to automate fish counting to make informed decisions while salmon are still running.” 

The Salmon Vision pilot study annotates more than 500,000 individual video frames captured at two Indigenous-run fish counting weirs on the Kitwanga and Bear Rivers of B.C.’s Central Coast. 

The first-of-its-kind deep learning computer model, developed in data partnership with the Gitanyow Fisheries Authority and Skeena Fisheries Commission, shows promising accuracy in identifying salmon species. It yielded mean average precision rates of 67.6 percent in tracking 12 different fish species passing through custom fish-counting boxes at the two weirs, with scores surpassing 90 and 80 percent for coho and sockeye salmon: two of the principal fish species targeted by First Nations, commercial, and recreational fishers. 

“When we envisioned providing fast grants for projects focused on Indigenous futurism and climate resilience, this is the type of project that we hoped would come our way,” says Dr. Keolu Fox, a professor at the University of California-San Diego, and one of several reviewers in an early crowdfunding round for the development of Salmon Vision. 

Collaborators on the model, funded by the British Columbia Salmon Recovery and Innovation Fund, include researchers and fisheries managers with Simon Fraser University and Douglas College computing sciences, the Pacific Salmon Foundation, Gitanyow Fisheries Authority, and the Skeena Fisheries Commission. Following these exciting early results, the next step is to expand the model with partner First Nations into a half-dozen new watersheds on B.C.’s North and Central Coast.

Real-time data on salmon returns is critical on several fronts. According to Dr. Atlas, many fisheries in British Columbia have been data-poor for decades. That leaves fisheries managers to base harvest numbers on early-season catch data, rather than the true number of salmon returning. Meanwhile, changing weather patterns, stream flows, and ocean conditions are creating more variable salmon returns: uncertainty that compounds the ongoing risks of overfishing already-vulnerable populations.

“Without real-time data on salmon returns, it’s extremely difficult to build climate-smart, responsive fisheries,” says Dr. Atlas. “Salmon Vision data collection and analysis can fill that information gap.” 

It’s a tool that he says will be invaluable to First Nation fisheries managers and other organizations both at the decision-making table—in providing better information to manage conservation risks and fishing opportunities—and in remote rivers across salmon country, where on-the-ground data collection is challenging and costly. 

The Salmon Vision team is implementing automated counting on a trial basis in several rivers around the B.C. North and Central Coasts in 2023. The goal is to provide reliable real-time count data by 2024.

This October 18, 2023 article by Ramona DeNies for the Wild Salmon Center (WSC) is nicely written although it does cover some of the same material seen in the news release, Note: A link has been removed,

Right now, in rivers across British Columbia’s Central Coast, we don’t know how many salmon are actually returning. At least, not until fishing seasons are over.

And yet, fisheries managers still have to make decisions. They have to make forecasts, modeled on data from the past. They have to set harvest targets for commercial and recreational fisheries. And increasingly, they have to make the call on emergency closures, when things start looking grim.

“On the north and central coast of BC, we’ve seen really wildly variable returns of salmon over the last decade,” says Dr. Will Atlas, Wild Salmon Center Senior Watershed Scientist. “With accelerating climate change, every year is unprecedented now. Yet from a fisheries management perspective, we’re still going into most seasons assuming that this year will look like the past.”

One answer, Dr. Atlas says, is “Salmon Vision.” Results from this first-of-its-kind technology—developed by WSC in data partnership with the Gitanyow Fisheries Authority and Skeena Fisheries Commission—were recently published in Frontiers in Marine Science.

There are embedded images in DeNies’ October 18, 2023 article; it’s where I found the video.

Here’s a link to and a citation for the paper,

Wild salmon enumeration and monitoring using deep learning empowered detection and tracking by William I. Atlas, Sami Ma, Yi Ching Chou, Katrina Connors, Daniel Scurfield, Brandon Nam, Xiaoqiang Ma, Mark Cleveland, Janvier Doire, Jonathan W. Moore, Ryan Shea, Jiangchuan Liu. Front. Mar. Sci., 20 September 2023 Volume 10 – 2023 DOI: https://doi.org/10.3389/fmars.2023.1200408

This paper appears to be open access.

Agriculture and gene editing … shades of the AquAdvantage salmon

Salmon are not the only food animals being genetically altered (more about that later in this post) we can now add cows, pigs, and more.

This November 15, 2018 article by Candice Choi on the Huffington Post website illustrates some of the excitement and terror associated with gene editing farm animals,

A company wants to alter farm animals by adding and subtracting genetic traits in a lab. It sounds like science fiction, but Recombinetics sees opportunity for its technology in the livestock industry.

But first, it needs to convince regulators that gene-edited animals are no different than conventionally bred ones. To make the technology appealing and to ease any fears that it may be creating Franken-animals, [emphasis mine] Recombinetics isn’t starting with productivity. Instead, it’s introducing gene-edited traits as a way to ease animal suffering.

“It’s a better story to tell,” said Tammy Lee, CEO of the St. Paul, Minnesota-based company.

For instance, animal welfare advocates have long criticized the way farmers use caustic paste or hot irons to dehorn dairy cows so the animals don’t harm each other. Recombinetics snips out the gene for growing horns so the procedure is unnecessary. [emphases mine]

Last year, a bull gene-edited by Recombinetics to have the dominant hornless trait sired several offspring. All were born hornless as expected, and are being raised at the University of California, Davis. Once the female offspring starts lactating, its milk will be tested for any abnormalities.

Another Recombinetics project: castration-free pigs.

When male piglets go through puberty, their meat can take on an unpleasant odour, something known as “boar taint.” To combat it, farmers castrate pigs, a procedure animal welfare advocates say is commonly performed without painkillers. Editing genes so that pigs never go through puberty would make castration unnecessary.

Also in development are dairy cows that could withstand higher temperatures, so the animals don’t suffer in hotter climates. [emphasis mine]

..

Before food from gene-edited animals can land on dinner tables, however, Recombinetics has to overcome any public unease about the technology.

Beyond worries about “playing God,” it may be an uncomfortable reminder of how modern food production already treats animals, said Paul Thompson, a professor of agriculture at Michigan State University.

“There’s an ethical question that’s been debated for at least the last 20 years, of whether you need to change the animal or change the system,” Thompson said.

Support for gene editing will also likely depend on how the technology is used: whether it’s for animal welfare, productivity or disease resistance. In August, a Pew study found 43 per cent of Americans supported genetically engineered animals for more nutritious meat.

Choi has written an interesting article, which includes a picture of the hornless cows embedded in the piece. One note: Choi makes reference to a milk glut. As far as I’m aware that’s not the case in Canada (at this time) but it is a problem in the US where in 2015 (?) farmers dumped some 43  million gallons of milk (October 12, 2016 article by Martha C. White for Money magazine).

As for the salmon, I’ve covered that story a few times during its journey to being approved for human consumption i Canada (my May 20, 2016 posting) to the discovery in 2017 that the genetically modified product, AquAdvantage salmon, had been introduced into the market, (from my Sept. 13, 2017 posting; scroll down about 40R of the way),

“Since the 2016 approval, AquAdvantage salmon, 4.5M tonnes has been sold in Canada according to an Aug. 8, 2017 article by Sima Shakeri for Huffington Post …”

After decades of trying to get approval by in North America, genetically modified Atlantic salmon has been sold to consumers in Canada.

AquaBounty Technologies, an American company that produces the Atlantic salmon, confirmed it had sold 4.5 tonnes of the modified fish on August 4 [2017], the Scientific American reported.

The fish have been engineered with a growth hormone gene from Chinook salmon to grow faster than regular salmon and require less food. They take about 18 months to reach market size, which is much quicker than the 30 months or so for conventional salmon.

The Washington Post wrote AquaBounty’s salmon also contains a gene from the ocean pout that makes the salmon produce the growth hormone gene all-year-round.

The company produces the eggs in a facility in P.E.I. [Prince Edward Island; a province in Canada], which is currently being expanded, and then they’re shipped to Panama where the fish are raised.

….

There was a bit of a kerfuffle about the whole affair but it seems Canadians have gone on to embrace the genetically modified product. At least that’s Christine Blank’s perspective in her Sept. 13, 2018 article (Canada, US embrace AquAdvantage GMO salmon, Brazil and China may be next) for the Genetic Literacy Project website,

Genetically modified salmon firm AquaBounty has found “very enthusiastic” buyers in Canada, according to president and CEO Ronald Stotish.

The first sale of the Maynard, Massachusetts, U.S.A.-based firm’s AquAdvantage salmon was made last June [2017], when unnamed buyers in Canada bought five metric tons at the going rate of traditional farmed Atlantic salmon, according to the company. Since then, AquaBounty has sold 10 additional metric tons of its AquAdvantage salmon to buyers in Canada

Meanwhile, Stotish revealed that AquAdvantage will be sold in the U.S. through established distributors.

“Once [AquaBounty salmon] is established in the market, the option for branding as a ‘sustainably produced’ food item can be considered,” he told investors.

Alex Gillis’ June 5, 2018 article for Macleans magazine suggests that Canadians may be a bit more doubtful about GM (genetically modified) salmon than Stotish seems to be believe,

An Ipsos Reid poll conducted for the Canadian Biotechnology Action Network in 2015 suggested that Canadians are concerned about GM foods, in spite of government assurances that they’re safe. About 60 per cent of respondents opposed genetically modifying crops and animals for food; nearly half supported a ban on all GM food. More than 20 years of surveys indicate that the vast majority of Canadians want to know when they’re eating GMOs. Fully 88 per cent of those polled in the 2015 survey said they want mandatory labelling.

Their concern hasn’t escaped the notice of those who raise and sell much of the salmon consumed in this country. Five years ago, Marine Harvest, one of the world’s largest producers of farmed salmon, called for labelling of GMOs. Today, it says that it doesn’t grow, sell or research GM salmon, a policy it shares with major salmon producers in Canada. And most big grocery retailers have stated they don’t want GM salmon. When contacted by Maclean’s for this story, Metro, Sobeys, Wal-Mart and Loblaws—four of Canada’s five largest food retailers—declared that none of AquaBounty’s GM salmon from 2017 was sold in their stores, saying neither Sea Delight Canada nor Montreal Fish Co. supplied them with Atlantic salmon at the time.

“I’m happy to report that we don’t source salmon from these two companies,” says Geneviève Grégoire, communications adviser with Metro Richelieu Inc., which operates or supplies 948 food stores in Quebec and Ontario, including Metro, Super C, Food Basics, Adonis and Première Moisson. “As we said before, we didn’t and will not sell GM Atlantic salmon.”

If you’re looking for a more comprehensive and critical examination of the issue, read Lucy Sharratt’s Sept. 1, 2018 article for the Canadian Centre for Policy Alternatives (CCPA).

DNA sunscreen: the longer you wear it, the better it gets due to its sacrificial skin

Using this new sunscreen does mean slathering on salmon sperm, more or lees, (read the Methods section of the academic paper cited later in this post). Considering that you’ve likely eaten (insect parts in chocolate) and slathered on more discomfiting stuff already and this development gives you access to an all natural, highly effective sunscreen, if it ever makes its way out of the laboratory, it might not be so bad. From a July 26, 2017 article by Sarah Knapton for The Telegraph,

Sunscreen made from DNA [deoxyribonucleic acid] which acts like a second skin to prevent sun damage is on the horizon.

Scientists in the US have developed a film from the DNA of salmon which gets better at protecting the skin from ultraviolet light the more it is exposed to the Sun.

It also helps lock in moisture beneath the surface which is usually lost during tanning.

Exciting, yes? A July 27, 2017 Binghamton University news release (also on EurekAlert but dated July 26, 2017) provides more detail,

“Ultraviolet (UV) light can actually damage DNA, and that’s not good for the skin,” said Guy German, assistant professor of biomedical engineering at Binghamton University. “We thought, let’s flip it. What happens instead if we actually used DNA as a sacrificial layer? So instead of damaging DNA within the skin, we damage a layer on top of the skin.”

German and a team of researchers developed thin and optically transparent crystalline DNA films and irradiated them with UV light. They found that the more they exposed the film to UV light, the better the film got at absorbing it.

“If you translate that, it means to me that if you use this as a topical cream or sunscreen, the longer that you stay out on the beach, the better it gets at being a sunscreen,” said German.

As an added bonus, the DNA coatings are also hygroscopic, meaning that skin coated with the DNA films can store and hold water much more than uncoated skin. When applied to human skin, they are capable of slowing water evaporation and keeping the tissue hydrated for extended periods of time.

German intends to see next if these materials might be good as a wound covering for hostile environments where 1) you want to be able to see the wound healing without removing the dressing, 2) you want to protect the wound from the sun and 3) you want to keep the wound in a moist environment, known to promote faster wound healing rates.

“Not only do we think this might have applications for sunscreen and moisturizers directly, but if it’s optically transparent and prevents tissue damage from the sun and it’s good at keeping the skin hydrated, we think this might be potentially exploitable as a wound covering for extreme environments,” he said.

Here’s a link to and a citation for the paper,

Non-ionising UV light increases the optical density of hygroscopic self assembled DNA crystal films by Alexandria E. Gasperini, Susy Sanchez, Amber L. Doiron, Mark Lyles & Guy K. German. Scientific Reports 7, Article number: 6631 (2017) doi:10.1038/s41598-017-06884-8 Published online: 26 July 2017

This paper is open access.

Injectable and more powerful* batteries for live salmon

Today’s live salmon may sport a battery for monitoring purposes and now scientists have developed one that is significantly more powerful according to a Feb. 17, 2014 Pacific Northwest National Laboratory (PNNL) news release (dated Feb. 18, 2014 on EurekAlert),

Scientists have created a microbattery that packs twice the energy compared to current microbatteries used to monitor the movements of salmon through rivers in the Pacific Northwest and around the world.

The battery, a cylinder just slightly larger than a long grain of rice, is certainly not the world’s smallest battery, as engineers have created batteries far tinier than the width of a human hair. But those smaller batteries don’t hold enough energy to power acoustic fish tags. The new battery is small enough to be injected into an organism and holds much more energy than similar-sized batteries.

Here’s a photo of the battery as it rests amongst grains of rice,

The microbattery created by Jie Xiao and Daniel Deng and colleagues, amid grains of rice. Courtesy PNNL

The microbattery created by Jie Xiao and Daniel Deng and colleagues, amid grains of rice. Courtesy PNNL

The news release goes on to explain why scientists are developing a lighter battery for salmon and how they achieved their goal,

For scientists tracking the movements of salmon, the lighter battery translates to a smaller transmitter which can be inserted into younger, smaller fish. That would allow scientists to track their welfare earlier in the life cycle, oftentimes in the small streams that are crucial to their beginnings. The new battery also can power signals over longer distances, allowing researchers to track fish further from shore or from dams, or deeper in the water.

“The invention of this battery essentially revolutionizes the biotelemetry world and opens up the study of earlier life stages of salmon in ways that have not been possible before,” said M. Brad Eppard, a fisheries biologist with the Portland District of the U.S. Army Corps of Engineers.

“For years the chief limiting factor to creating a smaller transmitter has been the battery size. That hurdle has now been overcome,” added Eppard, who manages the Portland District’s fisheries research program.

The Corps and other agencies use the information from tags to chart the welfare of endangered fish and to help determine the optimal manner to operate dams. Three years ago the Corps turned to Z. Daniel Deng, a PNNL engineer, to create a smaller transmitter, one small enough to be injected, instead of surgically implanted, into fish. Injection is much less invasive and stressful for the fish, and it’s a faster and less costly process.

“This was a major challenge which really consumed us these last three years,” said Deng. “There’s nothing like this available commercially, that can be injected. Either the batteries are too big, or they don’t last long enough to be useful. That’s why we had to design our own.”

Deng turned to materials science expert Jie Xiao to create the new battery design.

To pack more energy into a small area, Xiao’s team improved upon the “jellyroll” technique commonly used to make larger household cylindrical batteries. Xiao’s team laid down layers of the battery materials one on top of the other in a process known as lamination, then rolled them up together, similar to how a jellyroll is created. The layers include a separating material sandwiched by a cathode made of carbon fluoride and an anode made of lithium.

The technique allowed her team to increase the area of the electrodes without increasing their thickness or the overall size of the battery. The increased area addresses one of the chief problems when making such a small battery — keeping the impedance, which is a lot like resistance, from getting too high. High impedance occurs when so many electrons are packed into a small place that they don’t flow easily or quickly along the routes required in a battery, instead getting in each other’s way. The smaller the battery, the bigger the problem.

Using the jellyroll technique allowed Xiao’s team to create a larger area for the electrons to interact, reducing impedance so much that the capacity of the material is about double that of traditional microbatteries used in acoustic fish tags.

“It’s a bit like flattening wads of Play-Doh, one layer at a time, and then rolling them up together, like a jelly roll,” says Xiao. “This allows you to pack more of your active materials into a small space without increasing the resistance.”

The new battery is a little more than half the weight of batteries currently used in acoustic fish tags — just 70 milligrams, compared to about 135 milligrams — and measures six millimeters long by three millimeters wide. The battery has an energy density of about 240 watt hours per kilogram, compared to around 100 for commercially available silver oxide button microbatteries.

The battery holds enough energy to send out an acoustic signal strong enough to be useful for fish-tracking studies even in noisy environments such as near large dams. The battery can power a 744-microsecond signal sent every three seconds for about three weeks, or about every five seconds for a month. It’s the smallest battery the researchers know of with enough energy capacity to maintain that level of signaling.

The batteries also work better in cold water where salmon often live, sending clearer signals at low temperatures compared to current batteries. That’s because their active ingredients are lithium and carbon fluoride, a chemistry that is promising for other applications but has not been common for microbatteries.

Last summer in Xiao’s laboratory, scientists Samuel Cartmell and Terence Lozano made by hand more than 1,000 of the rice-sized batteries. It’s a painstaking process, cutting and forming tiny snippets of sophisticated materials, putting them through a flattening device that resembles a pasta maker, binding them together, and rolling them by hand into tiny capsules. Their skilled hands rival those of surgeons, working not with tissue but with sensitive electronic materials.

A PNNL team led by Deng surgically implanted 700 of the tags into salmon in a field trial in the Snake River last summer. Preliminary results show that the tags performed extremely well. The results of that study and more details about the smaller, enhanced fish tags equipped with the new microbattery will come out in a forthcoming publication. Battelle, which operates PNNL, has applied for a patent on the technology.

I notice that while the second paragraph of the news release (in the first excerpt) says the battery is injectable, the final paragraph (in the second excerpt) says the team “surgically implanted” the tags with their new batteries into the salmon.

Here’s a link to and a citation for the newly published article in Scientific Reports,

Micro-battery Development for Juvenile Salmon Acoustic Telemetry System Applications by Honghao Chen, Samuel Cartmell, Qiang Wang, Terence Lozano, Z. Daniel Deng, Huidong Li, Xilin Chen, Yong Yuan, Mark E. Gross, Thomas J. Carlson, & Jie Xiao. Scientific Reports 4, Article number: 3790 doi:10.1038/srep03790 Published 21 January 2014

This paper is open access.

* I changed the headline from ‘Injectable batteries for live salmon made more powerful’ to ‘Injectable and more powerful batteries for live salmon’  to better reflect the information in the news release. Feb. 19, 2014 at 11:43 am PST.

ETA Feb. 20, 2014: Dexter Johnson has weighed in on this very engaging and practical piece of research in a Feb. 19, 2014 posting on his Nanoclast blog (on the IEEE [Institute of Electrical and Electronics Engineers]) website (Note: Links have been removed),

There’s no denying that building the world’s smallest battery is a notable achievement. But while they may lay the groundwork for future battery technologies, today such microbatteries are mostly laboratory curiosities.

Developing a battery that’s no bigger than a grain of rice—and that’s actually useful in the real world—is quite another kind of achievement. Researchers at Pacific Northwest National Laboratory (PNNL) have done just that, creating a battery based on graphene that has successfully been used in monitoring the movements of salmon through rivers.

The microbattery is being heralded as a breakthrough in biotelemetry and should give researchers never before insights into the movements and the early stages of life of the fish.

The battery is partly made from a fluorinated graphene that was described last year …