When discussing DNA (deoxyribonucleic acid) one doesn’t usually expect to encounter gems as one does in a Nov. 28, 2013 news item on Azonano,
Nature builds flawless diamonds, sapphires and other gems. Now a Northwestern University [located in Chicago, Illinois, US] research team is the first to build near-perfect single crystals out of nanoparticles and DNA, using the same structure favored by nature.
The Nov. 27, 2013 Northwestern University news release by Megan Fellman (also on EurekAlert), which originated the news item,, explains why single crystals are of such interest,
“Single crystals are the backbone of many things we rely on — diamonds for beauty as well as industrial applications, sapphires for lasers and silicon for electronics,” said nanoscientist Chad A. Mirkin. “The precise placement of atoms within a well-defined lattice defines these high-quality crystals.
“Now we can do the same with nanomaterials and DNA, the blueprint of life,” Mirkin said. “Our method could lead to novel technologies and even enable new industries, much as the ability to grow silicon in perfect crystalline arrangements made possible the multibillion-dollar semiconductor industry.”
His research group developed the “recipe” for using nanomaterials as atoms, DNA as bonds and a little heat to form tiny crystals. This single-crystal recipe builds on superlattice techniques Mirkin’s lab has been developing for nearly two decades.
(I wrote about Mirkin’s nanoparticle DNA work in the context of his proposed periodic table of modified nucleic acid nanoparticles in a July 5, 2013 posting.) The news release goes on to describe Mirkin’s most recent work,
In this recent work, Mirkin, an experimentalist, teamed up with Monica Olvera de la Cruz, a theoretician, to evaluate the new technique and develop an understanding of it. Given a set of nanoparticles and a specific type of DNA, Olvera de la Cruz showed they can accurately predict the 3-D structure, or crystal shape, into which the disordered components will self-assemble.
The general set of instructions gives researchers unprecedented control over the type and shape of crystals they can build. The Northwestern team worked with gold nanoparticles, but the recipe can be applied to a variety of materials, with potential applications in the fields of materials science, photonics, electronics and catalysis.
A single crystal has order: its crystal lattice is continuous and unbroken throughout. The absence of defects in the material can give these crystals unique mechanical, optical and electrical properties, making them very desirable.
In the Northwestern study, strands of complementary DNA act as bonds between disordered gold nanoparticles, transforming them into an orderly crystal. The researchers determined that the ratio of the DNA linker’s length to the size of the nanoparticle is critical.
“If you get the right ratio it makes a perfect crystal — isn’t that fun?” said Olvera de la Cruz, who also is a professor of chemistry in the Weinberg College of Arts and Sciences. “That’s the fascinating thing, that you have to have the right ratio. We are learning so many rules for calculating things that other people cannot compute in atoms, in atomic crystals.”
The ratio affects the energy of the faces of the crystals, which determines the final crystal shape. Ratios that don’t follow the recipe lead to large fluctuations in energy and result in a sphere, not a faceted crystal, she explained. With the correct ratio, the energies fluctuate less and result in a crystal every time.
“Imagine having a million balls of two colors, some red, some blue, in a container, and you try shaking them until you get alternating red and blue balls,” Mirkin explained. “It will never happen.
“But if you attach DNA that is complementary to nanoparticles — the red has one kind of DNA, say, the blue its complement — and now you shake, or in our case, just stir in water, all the particles will find one another and link together,” he said. “They beautifully assemble into a three-dimensional crystal that we predicted computationally and realized experimentally.”
To achieve a self-assembling single crystal in the lab, the research team reports taking two sets of gold nanoparticles outfitted with complementary DNA linker strands. Working with approximately 1 million nanoparticles in water, they heated the solution to a temperature just above the DNA linkers’ melting point and then slowly cooled the solution to room temperature, which took two or three days.
The very slow cooling process encouraged the single-stranded DNA to find its complement, resulting in a high-quality single crystal approximately three microns wide. “The process gives the system enough time and energy for all the particles to arrange themselves and find the spots they should be in,” Mirkin said.
The researchers determined that the length of DNA connected to each gold nanoparticle can’t be much longer than the size of the nanoparticle. In the study, the gold nanoparticles varied from five to 20 nanometers in diameter; for each, the DNA length that led to crystal formation was about 18 base pairs and six single-base “sticky ends.”
“There’s no reason we can’t grow extraordinarily large single crystals in the future using modifications of our technique,” said Mirkin, who also is a professor of medicine, chemical and biological engineering, biomedical engineering and materials science and engineering and director of Northwestern’s International Institute for Nanotechnology.
Here’s a link to and a citation for the published paper,
DNA-mediated nanoparticle crystallization into Wulff polyhedra by Evelyn Auyeung, Ting I. N. G. Li, Andrew J. Senesi, Abrin L. Schmucker, Bridget C. Pals, Monica Olvera de la Cruz, & Chad A. Mirkin. Nature (2013) doi:10.1038/nature12739 Published online 27 November 2013
This article is behind a paywall.
Points to anyone who recognized the song title (Bewitched, Bothered and Bewildered) embedded in the head for this posting.