Monthly Archives: February 2022

Fridge-free COVID-19 vaccines?

COVID-19 vaccines require cold storage conditions (in some cases, extraordinarily cold storage), which pose problems with both storage and distribution.

A September 7, 2021 news item on phys.org describes research that may make vaccine distribution and storage problems a thing of the past (Note: A link has been removed),

Nanoengineers at the University of California San Diego have developed COVID-19 vaccine candidates that can take the heat. Their key ingredients? Viruses from plants or bacteria.

The new fridge-free COVID-19 vaccines are still in the early stage of development. In mice, the vaccine candidates triggered high production of neutralizing antibodies against SARS-CoV-2, the virus that causes COVID-19. If they prove to be safe and effective in people, the vaccines could be a big game changer for global distribution efforts, including those in rural areas or resource-poor communities.

A September 7, 2021 University of California at San Diego (UCSD or UC San Diego) news release (also on EurekAlert), which originated the news item, delves further into the research,

“What’s exciting about our vaccine technology is that is thermally stable, so it could easily reach places where setting up ultra-low temperature freezers, or having trucks drive around with these freezers, is not going to be possible,” said Nicole Steinmetz, a professor of nanoengineering and the director of the Center for Nano-ImmunoEngineering at the UC San Diego Jacobs School of Engineering.

The vaccines are detailed in a paper published Sept. 7 [2021] in the Journal of the American Chemical Society.

The researchers created two COVID-19 vaccine candidates. One is made from a plant virus, called cowpea mosaic virus. The other is made from a bacterial virus, or bacteriophage, called Q beta.

Both vaccines were made using similar recipes. The researchers used cowpea plants and E. coli bacteria to grow millions of copies of the plant virus and bacteriophage, respectively, in the form of ball-shaped nanoparticles. The researchers harvested these nanoparticles and then attached a small piece of the SARS-CoV-2 spike protein to the surface. The finished products look like an infectious virus so the immune system can recognize them, but they are not infectious in animals and humans. The small piece of the spike protein attached to the surface is what stimulates the body to generate an immune response against the coronavirus.

The researchers note several advantages of using plant viruses and bacteriophages to make their vaccines. For one, they can be easy and inexpensive to produce at large scales. “Growing plants is relatively easy and involves infrastructure that’s not too sophisticated,” said Steinmetz. “And fermentation using bacteria is already an established process in the biopharmaceutical industry.”

Another big advantage is that the plant virus and bacteriophage nanoparticles are extremely stable at high temperatures. As a result, the vaccines can be stored and shipped without needing to be kept cold. They also can be put through fabrication processes that use heat. The team is using such processes to package their vaccines into polymer implants and microneedle patches. These processes involve mixing the vaccine candidates with polymers and melting them together in an oven at temperatures close to 100 degrees Celsius. Being able to directly mix the plant virus and bacteriophage nanoparticles with the polymers from the start makes it easy and straightforward to create vaccine implants and patches. 

The goal is to give people more options for getting a COVID-19 vaccine and making it more accessible. The implants, which are injected underneath the skin and slowly release vaccine over the course of a month, would only need to be administered once. And the microneedle patches, which can be worn on the arm without pain or discomfort, would allow people to self-administer the vaccine.

“Imagine if vaccine patches could be sent to the mailboxes of our most vulnerable people, rather than having them leave their homes and risk exposure,” said Jon Pokorski, a professor of nanoengineering at the UC San Diego Jacobs School of Engineering, whose team developed the technology to make the implants and microneedle patches.

“If clinics could offer a one-dose implant to those who would have a really hard time making it out for their second shot, that would offer protection for more of the population and we could have a better chance at stemming transmission,” added Pokorski, who is also a founding faculty member of the university’s Institute for Materials Discovery and Design.

In tests, the team’s COVID-19 vaccine candidates were administered to mice either via implants, microneedle patches, or as a series of two shots. All three methods produced high levels of neutralizing antibodies in the blood against SARS-CoV-2.

Potential Pan-Coronavirus Vaccine

These same antibodies also neutralized against the SARS virus, the researchers found.

It all comes down to the piece of the coronavirus spike protein that is attached to the surface of the nanoparticles. One of these pieces that Steinmetz’s team chose, called an epitope, is almost identical between SARS-CoV-2 and the original SARS virus.

“The fact that neutralization is so profound with an epitope that’s so well conserved among another deadly coronavirus is remarkable,” said co-author Matthew Shin, a nanoengineering Ph.D. student in Steinmetz’s lab. “This gives us hope for a potential pan-coronavirus vaccine that could offer protection against future pandemics.”

Another advantage of this particular epitope is that it is not affected by any of the SARS-CoV-2 mutations that have so far been reported. That’s because this epitope comes from a region of the spike protein that does not directly bind to cells. This is different from the epitopes in the currently administered COVID-19 vaccines, which come from the spike protein’s binding region. This is a region where a lot of the mutations have occurred. And some of these mutations have made the virus more contagious.

Epitopes from a nonbinding region are less likely to undergo these mutations, explained Oscar Ortega-Rivera, a postdoctoral researcher in Steinmetz’s lab and the study’s first author. “Based on our sequence analyses, the epitope that we chose is highly conserved amongst the SARS-CoV-2 variants.”

This means that the new COVID-19 vaccines could potentially be effective against the variants of concern, said Ortega-Rivera, and tests are currently underway to see what effect they have against the Delta variant, for example.

Plug and Play Vaccine

Another thing that gets Steinmetz really excited about this vaccine technology is the versatility it offers to make new vaccines. “Even if this technology does not make an impact for COVID-19, it can be quickly adapted for the next threat, the next virus X,” said Steinmetz.

Making these vaccines, she says, is “plug and play:” grow plant virus or bacteriophage nanoparticles from plants or bacteria, respectively, then attach a piece of the target virus, pathogen, or biomarker to the surface.

“We use the same nanoparticles, the same polymers, the same equipment, and the same chemistry to put everything together. The only variable really is the antigen that we stick to the surface,” said Steinmetz.

The resulting vaccines do not need to be kept cold. They can be packaged into implants or microneedle patches. Or, they can be directly administered in the traditional way via shots.

Steinmetz and Pokorski’s labs have used this recipe in previous studies to make vaccine candidates for diseases like HPV and cholesterol. And now they’ve shown that it works for making COVID-19 vaccine candidates as well.

Next Steps

The vaccines still have a long way to go before they make it into clinical trials. Moving forward, the team will test if the vaccines protect against infection from COVID-19, as well as its variants and other deadly coronaviruses, in vivo.

Here’s a link to and a citation for the paper,

Trivalent Subunit Vaccine Candidates for COVID-19 and Their Delivery Devices by Oscar A. Ortega-Rivera, Matthew D. Shin, Angela Chen, Veronique Beiss, Miguel A. Moreno-Gonzalez, Miguel A. Lopez-Ramirez, Maria Reynoso, Hong Wang, Brett L. Hurst, Joseph Wang, Jonathan K. Pokorski, and Nicole F. Steinmetz. J. Am. Chem. Soc. 2021, XXXX, XXX, XXX-XXX DOI: https://doi.org/10.1021/jacs.1c06600 Publication Date:September 7, 2021 © 2021 American Chemical Society

This paper is behind a paywall.

Smart dental implant resists bacterial growth and generates own electricity

A “smart” dental implant could improve upon current devices by employing biofilm-resisting nanoparticles and a light powered by biomechanical forces to promote health of the surrounding gum tissue. (Image: Courtesy of Albert Kim)

A September 9, 2021 news item on ScienceDaily announces research into ‘smart’ dental implants,

More than 3 million people in America have dental implants, used to replace a tooth lost to decay, gum disease, or injury. Implants represent a leap of progress over dentures or bridges, fitting much more securely and designed to last 20 years or more.

But often implants fall short of that expectation, instead needing replacement in five to 10 years due to local inflammation or gum disease, necessitating a repeat of a costly and invasive procedure for patients.

“We wanted to address this issue, and so we came up with an innovative new implant,” says Geelsu Hwang, an assistant professor in the University of Pennsylvania School of Dental Medicine, who has a background in engineering that he brings to his research on oral health issues.

The novel implant would implement two key technologies, Hwang says. One is a nanoparticle-infused material that resists bacterial colonization. And the second is an embedded light source to conduct phototherapy, powered by the natural motions of the mouth, such as chewing or toothbrushing. In a paper in the journal ACS Applied Materials & Interfaces and a 2020 paper in the journal Advanced Healthcare Materials, Hwang and colleagues lay out their platform, which could one day be integrated not only into dental implants but other technologies, such as joint replacements, as well.

A September 9, 2021 University of Pennsylvania news release (also on EurekAlert), which originated the news item, provides more technical details about the proposed technology,

“Phototherapy can address a diverse set of health issues,” says Hwang. “But once a biomaterial is implanted, it’s not practical to replace or recharge a battery. We are using a piezoelectric material, which can generate electrical power from natural oral motions to supply a light that can conduct phototherapy, and we find that it can successfully protect gingival tissue from bacterial challenge.”

In the paper, the material the researchers explored was barium titanate (BTO), which has piezoelectric properties that are leveraged in applications such as capacitators and transistors, but has not yet been explored as a foundation for anti-infectious implantable biomaterials. To test its potential as the foundation for a dental implant, the team first used discs embedded with nanoparticles of BTO and exposed them to Streptococcus mutans, a primary component of the bacterial biofilm responsible for tooth decay commonly known as dental plaque. They found that the discs resisted biofilm formation in a dose-dependent manner. Discs with higher concentrations of BTO were better at preventing biofilms from binding.

While earlier studies had suggested that BTO might kill bacteria outright using reactive oxygen species generated by light-catalyzed or electric polarization reactions, Hwang and colleagues did not find this to be the case due to the short-lived efficacy and off-target effects of these approaches. Instead, the material generates enhanced negative surface charge that repels the negatively charged cell walls of bacteria. It’s likely that this repulsion effect would be long-lasting, the researchers say.

“We wanted an implant material that could resist bacterial growth for a long time because bacterial challenges are not a one-time threat,” Hwang says.

The power-generating property of the material was sustained and in tests over time the material did not leach. It also demonstrated a level of mechanical strength comparable to other materials used in dental applications.

Finally, the material did not harm normal gingival tissue in the researchers’ experiments, supporting the idea that this could be used without ill effect in the mouth.

The technology is a finalist in the Science Center’s research accelerator program, the QED Proof-of-Concept program. As one of 12 finalists, Hwang and colleagues will receive guidance from experts in commercialization. If the project advances to be one of three finalists, the group has the potential to receive up to $200,000 in funding.

In future work, the team hopes to continue to refine the “smart” dental implant system, testing new material types and perhaps even using assymetric properties on each side of the implant components, one that encourages tissue integration on the side facing the gums and one that resists bacterial formation on the side facing the rest of the mouth.

“We hope to further develop the implant system and eventually see it commercialized so it can be used in the dental field,” Hwang says.

Here’s a link to and a citation for the paper,

Bimodal Nanocomposite Platform with Antibiofilm and Self-Powering Functionalities for Biomedical Applications by Atul Dhall, Sayemul Islam, Moonchul Park, Yu Zhang, Albert Kim, and Geelsu Hwang. ACS Appl. Mater. Interfaces 2021, 13, 34, 40379–40391 DOI: https://doi.org/10.1021/acsami.1c11791 Publication Date:August 18, 2021 Copyright © 2021 American Chemical Society

This paper is behind a paywall.

The work from 2020, mentioned in the news release, laid groundwork for the latest paper.

Human Oral Motion-Powered Smart Dental Implant (SDI) for In Situ Ambulatory Photo-biomodulation Therapy by Moonchul Park, Sayemul Islam, Hye-Eun Kim, Jonathan Korosto, Markus B. Blatz, Geelsu Hwang, and Albert Kim. Adv. Healthcare Mater. 2020, 9, 2000658 DOI: 10.1002/adhm.202000658 First published: 01 July 2020 © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimHuman

This paper is behind a paywall.

Nobel Laureates write science articles for children

Caption: Frontiers for Young Minds Nobel Collection. Credit: Frontiers Media

A September 7, 2021 Frontiers news release (also on EurekAlert) describes the company’s latest initiative to engage children in science (Note: I have a bit more about one of the Nobel Laureates, Dan [Daniel] Schechtman at the end of this posting),

Young people everywhere now have access to a free collection of scientific articles written by winners of science’s most coveted honor, the Nobel Prize. The Nobel Collection, published by Frontiers, aims to improve young people’s access to learning material about science’s role in addressing today’s global challenges. The collection will connect young minds with some of today’s most distinguished scientists through engaging learning material steeped in some of the most groundbreaking research from over the last twenty years.

Written for young people aged eight to 15, the collection has been published in the journal Frontiers for Young Minds. With the help of a science mentor, each article in the Nobel Collection has been reviewed by kids themselves to ensure it is understandable, fun, and engaging before publication. By sparking an interest in science from a young age, the Nobel Collection aims to improve young people’s scientific worldview. Its objective is to equip them with a scientific mindset and appreciation of the central role of science in finding solutions to today’s growing catalogue of global challenges.

A keen 13-year-old reviewer from Switzerland shared his experience, “I’m very interested in science and it is fascinating to review papers from the real scientists who know so much about their specialized fields! Many of the papers explain dangerous illnesses to children, and I think such information is so important!”

May-Britt Moser, awarded The Nobel Prize in Physiology or Medicine 2014, said, “I’m honored to contribute to the journal Frontiers for Young Minds. Children are born curious, with passion for questions and with light in their eyes. As a scientist, I feel privileged to be able to ask questions that I think are important. I hope the papers in this journal may help nurture and reinforce children’s passion and curiosity for science – what a gift to humanity that would be!”

Commenting on the Collection, Aaron Ciechanover who was awarded The Nobel Prize in Chemistry 2004, said, “Prizes and recognition are not targets that one should aim for. Breakthrough achievements that expand our knowledge of the world and benefit mankind are. Reading about science was my hobby as a kid and, doubtless, the seed of my curiosity into scientific discovery.”

Currently, the Nobel Collection comprises of contributions including:

How do we find our way? Grid cells in the brain, written by May-Britt Moser, awarded The Nobel Prize in Physiology or Medicine 2014.

Computer Simulations in Service of Biology, written by Michael Levitt, awarded The Nobel Prize in Chemistry 2013.

Quasi-Crystal, Not Quasi-Scientist, written by Dan Shechtman, awarded The Nobel Prize in Chemistry 2011.

The Transcription of Life: from DNA to RNA, written by Roger D. Kornberg, awarded The Nobel Prize in Chemistry 2006.

Targeted Degradation of Proteins – the Ubiquitin System, written by Aaron Ciechanover, The Nobel Prize in Chemistry 2004.

The Nobel Collection’s co-editor Idan Segev, professor of computational neuroscience at the Hebrew University, said: “What we want to achieve with this collection, beyond improving kids’ understanding of the scientific process and the particular Nobel recognized breakthroughs, is to acquaint kids with scientific role models – someone for young people to look up to. The beauty of these articles is that the Nobel Laureates share their life experience with kids, their failures and passions, and provide personal advice for the young minds.

“The kids that we worked with to review the articles were amazed by what they were reading and left the classes with a real sense of admiration for the humanistic as well as the scientific facet of Nobel prize winners. It is an incredible learning resource that can be accessed by anyone with an internet connection worldwide, which in context of the disruption created by the COVID-19 pandemic makes it particularly important.”  

UN Sustainable Development Goals – Quality Education

The initiative is also part of Frontiers’ commitment to the United Nations Sustainable Development Goals [SDGs], particularly Goal 4 – Quality Education. Disruption to access to quality education has been exacerbated by the COVID-19 pandemic, potentially jeopardizing some of the hard-won gains in recent years.

Frontiers, who funds the Frontiers for Young Minds journal as part of its philanthropy program, intends to work with at least five more Nobel Laureates later this year to grow the resource. All the articles are free to read, download, and share. Plans are also in place to translate the Nobel Collection into a portfolio of languages so even more young people from around the world can make use of it.

Dr. Fred Fenter, chief executive editor of Frontiers, said: “From fighting climate change to disease to poverty, science saves lives. What better role models to inspire future generations of scientists than Nobel Prize winners themselves. Our hope is the Nobel Collection will act as a catalyst, both motivating young people and improving their appreciation of the central role science will play in creating a sustainable future for people and planet.”

The Frontiers for Young Minds initiative

The Frontiers for Young Minds journal launched in 2013. Since then, Frontiers has engaged with around 3,500 young reviewers, each of whom has been guided by one of around 600 science mentors. To date, the journal has received more than ten million views and downloads of its 750 articles, which include English, Hebrew, and Arabic versions. The Frontiers for Young Minds editorial board currently consists of scientists and researchers from more than 64 countries.

Topics included in the journal range from astronomy and space science to biodiversity, neuroscience, pollution prevention, and mental health. Although written and edited for a younger audience, all the research published in Frontiers for Young Minds is based on solid evidence-based scientific research. 

I found the Schechtman story in my December 24, 2013 posting,

I suggested earlier that this achievement has a fabulous quality and the Daniel Schechtman backstory is the reason. The winner of the 2011 Nobel Prize for Chemistry, Schechtman was reviled for years within his scientific community as Ian Sample notes in his Oct. 5, 2011 article on the announcement of Schechtman’s Nobel win written for the Guardian newspaper (Note: A link has been removed),

“A scientist whose work was so controversial he was ridiculed and asked to leave his research group has won the Nobel Prize in Chemistry.

Daniel Shechtman, 70, a researcher at Technion-Israel Institute of Technology in Haifa, received the award for discovering seemingly impossible crystal structures in frozen gobbets of metal that resembled the beautiful patterns seen in Islamic mosaics.

Images of the metals showed their atoms were arranged in a way that broke well-establised rules of how crystals formed, a finding that fundamentally altered how chemists view solid matter.

On the morning of 8 April 1982, Shechtman saw something quite different while gazing at electron microscope images of a rapidly cooled metal alloy. The atoms were packed in a pattern that could not be repeated. Shechtman said to himself in Hebrew, “Eyn chaya kazo,” which means “There can be no such creature.”

The bizarre structures are now known as “quasicrystals” and have been seen in a wide variety of materials. Their uneven structure means they do not have obvious cleavage planes, making them particularly hard.

In an interview this year with the Israeli newspaper, Haaretz, Shechtman said: “People just laughed at me.” He recalled how Linus Pauling, a colossus of science and a double Nobel laureate, mounted a frightening “crusade” against him. After telling Shechtman to go back and read a crystallography textbook, the head of his research group asked him to leave for “bringing disgrace” on the team. “I felt rejected,” Shachtman said.”

It takes a lot to persevere when most, if not all, of your colleagues are mocking and rejecting your work so bravo to Schechtman! And,bravo to the Japan-UK project researchers who have persevered to help solve at least part of a complex problem requiring that our basic notions of matter be rethought.

I encourage you to read Sample’s article in its entirety as it is well written and I’ve excerpted only bits of the story as it relates to a point I’m making in this post, i.e., perseverance in the face of extreme resistance.

Shechtman’s quasi-crystal story for Frontiers provides clear explanations and a little inspiration while not flinching away from the difficulties posed when shaking up established theories.

BTW, I like reading material written for children as there are often useful explanations that aren’t included in material intended for adults.

Sticky tape, hackers, and quantum communications

I always appreciate a low technology solution to a problem. In this case, it’s a piece of sticky tape which halts compute hackers in their tracks. Here’s more from an August 30, 2021 University of Technology Sydney press release (also on EurekAlert but published August 26, 2021), Note: Links have been removed,

Researchers from the University of Technology Sydney (UTS) and TMOS, an Australian Research Council Centre of Excellence [specifically, the Australian Research Council Centre of Excellence for Transformative Meta-Optical Systems (TMOS)], have taken the fight to online hackers with a giant leap towards realizing affordable, accessible quantum communications, a technology that would effectively prevent the decryption of online activity. Everything from private social media messaging to banking could become more secure due to new technology created with a humble piece of adhesive tape.

Quantum communication is still in its early development and is currently feasible only in very limited fields due to the costs associated with fabricating the required devices. The TMOS researches have developed new technology that integrates quantum sources and waveguides on chip in a manner that is both affordable and scalable, paving the way for future everyday use.

The development of fully functional quantum communication technologies has previously been hampered by the lack of reliable quantum light sources that can encode and transmit the information.

In a paper published today in ACS Photonics, the team describes a new platform to generate these quantum emitters based on hexagonal boron nitride, also known as white graphene. Where current quantum emitters are created using complex methods in expensive clean rooms, these new quantum emitters can be created using $20 worth of white graphene pressed on to a piece of adhesive tape.

These 2D materials can be pressed onto a sticky surface such as the [sic] adhesive tape [emphasis mine] and exfoliated, which is essentially peeling off the top layer to create a flex. Multiple layers of this flex can then be assembled in a Lego-like style, offering a new bottom up approach as a substitute for 3D systems.

TMOS Chief Investigator Igor Aharonovich said: “2D materials, like hexagonal boron nitride, are emerging materials for integrated quantum photonics, and are poised to impact the way we design and engineer future optical components for secured communication.”

In addition to this evolution in photon sources, the team has developed a high efficiency on-chip waveguide, a vital component for on-chip optical processing.

Lead author Chi Li said: “Low signal levels have been a significant barrier preventing quantum communications from evolving into practical, workable models. We hope that with this new development, quantum comms will become an everyday technology that improves people’s lives in new and exciting ways.”

Here’s a link to and a citation for the paper,

Integration of hBN Quantum Emitters in Monolithically Fabricated Waveguides by Chi Li, Johannes E. Fröch, Milad Nonahal, Thinh N. Tran, Milos Toth, Sejeong Kim, and Igor Aharonovich. ACS Photonics 2021, XXXX, XXX, XXX-XXX DOI: https://doi.org/10.1021/acsphotonics.1c00890 Publication Date:August 20, 2021 © 2021 American Chemical Society

This paper is behind a paywall.

Sticky or adhesive tape is part of graphene lore and seems to exert a great fascination for scientists as I note in my June 12, 2018 posting.

Phytoremediation with lupin and arsenic

Is anyone else reminded of Arsène Lupin? (More about Lupin later in this posing)

An August 24, 2021 news item on ScienceDaily describes research on soils and phytoremediation (decontamination by plants),

Pollution of soils with highly toxic arsenic is a worldwide problem generating substantial risks to human health and the environment.

In Canada, over 7000 sites contaminated with metals such as arsenic are considered ‘highly concerning’ by the government, with some past and recent mining operations and wood preservative facilities having left their mark on the environment by increasing soil arsenic levels by up to 1000 times the maximum regulatory health limits.

One way in which arsenic contaminated soils could be rejuvenated is to exploit natural mechanisms which have evolved in certain plants for contamination tolerance.

“The legume crop white lupin (L. albus) is one such arsenic tolerant plant species being studied as for sustainable remediation,” explains Adrien Frémont, lead author of the study and a doctoral student in biological sciences at the Université de Montréal. “The mechanism behind arsenic tolerance in white lupin is thought to be the release of plant chemicals directly into soil by roots, but the nature of these compounds is unknown and hard to study due to the complexity of these belowground interactions.”

Caption: The legume crop white lupin (L. albus) is one such arsenic tolerant plant species being studied as for sustainable remediation. Credit: UMONTREAL

An August 24, 2021 University of Montreal (Université de Montréal) news release (also on EurekAlert), which originated the news item, describes the work in more detail,

Root chemicals an undiscovered country

To study this, the team developed nylon pouches which could be placed close to roots in soil to capture exuded molecules without damaging the root system. The complex mix of molecules collected from these pouches were analysed using advanced (metabolomic) chemical profiling to identify the compounds capable of binding metals produced by the Lupin plants in response to high concentrations of arsenic. Some of these metal-binding molecules, phytochelatins, are known to be used internally by plants to deal with metal stress but have never before been captured as exuded into polluted soils.

“We’re really excited to see how matching new root-soil sampling approaches with advanced metabolomic profiling can yield such unexpected discoveries”, notes Frémont. “We know that plants can drastically change soil properties and can transform or immobilise soil pollution, but the chemistry underlying how they achieve this, and in particular the makeup and function of root-exuded compounds, is still very much an undiscovered country.”
 

Plant roots directly altering polluted soils

The next steps of the research are to branch out into more detailed analysis of the precise chemical reactions taking place at the root-soil interface, including exploration of different plant species, interactions with microorganisms and the challenge of diverse soil pollution.

As Dr. Nicholas Brereton, University of Montreal and the study’s senior author, mentions: “It can be a real challenge to research the complex interactions going on belowground between plants and soil, but these findings are rewarding in telling us that natural mechanisms have evolved in plants to deal with this type of pollution. Although we’re still only just beginning to scratch below the surface of how these plant root strategies work, as we learn more, we can potentially utilise these natural processes to improve soil health and help to alleviate some of the most persistent anthropogenic damage to our environment.”

Here’s a link to and a citation for the paper,

Phytochelatin and coumarin enrichment in root exudates of arsenic-treated white lupin by Adrien Frémont, Eszter Sas, Mathieu Sarrazin, Emmanuel Gonzalez, Jacques Brisson, Frédéric Emmanuel Pitre, Nicholas James Beresford Brereton. Plant Cell & Environment DOI: https://doi.org/10.1111/pce.14163 First published: 15 August 2021

This paper is behind a paywall.

For anyone interested in phytoremediation, I have a March 30, 2012 posting about it and there’s this Wikipedia entry. Depending on the circumstances, you might also consider phytoremediation as a form of phyto-mining, i.e., using plants to recover metals from mine tailings (see my March 5, 2013 posting).

Arsène Lupin

There are two of them (the first and the latest ones) being mentioned here; but there are many versions of Arsène Lupin in manga, anime, movies, etc.

The first fictional Arsène Lupin was created in 1905 by Maurice Leblanc. Here’s a description (on the Normandie tourisme website) of the first Lupin in an article about the latest Lupin, a series streamed on Netflix.

Maurice Leblanc was born in Rouen in 1864. Fascinated by legends of witches, Celts, Romans and the Vikings growing up, Leblanc would go on to develop a deep knowledge of and love for the region around Rouen, known as the Pays de Caux. After working in a factory in Rouen by day, writing only in his spare time, Leblanc eventually left his home town to study in Paris, where he then started working as a journalist for several publications including Le Figaro. Fate then struck, as publisher Pierre Lafitte launched the magazine Je sais tout and commissioned Leblanc to write a series of new crime stories where the hero would be a sort of French anti-Sherlock Holmes.

Who was the original Lupin? Not unlike Lupin in the TV series, the Arsène Lupin of the books was a thief, a master of disguise, a rascal but never a killer, a hit with the ladies and a righter of wrongs who takes from the rich, a French Robin Hood if you like. He takes on a multitude of personas in the books, constantly changing his looks and his name – examples include Prince Paul Sernine, Raoul d’Andrésy, Horace Velmont and Don Luis Perenna. In the [Lupin] series [2021], this is echoed by Assane’s alter-egos Paul Sernine, Luis Perenna and Salvatore813, as well as his choice of name for his son, Raoul. Yet superman Lupin, both in the books and on screen, always manages to triumph somehow over his enemies, even when all seems lost, through bending the rules, outsmarting the police and sheer self-belief.

You can find out more about the latest Lupin in its IMDb entry,

Inspired by the adventures of Arsène Lupin, gentleman thief Assane Diop sets out to avenge his father for an injustice inflicted by a wealthy family.

The television series starring Omar Sy was a huge hit in France and has been seen worldwide.

Nanopore-tal enables cells to talk to computers?

An August 25, 2021 news item on ScienceDaily announced research that will allow more direct communication between cells and computers,

Genetically encoded reporter proteins have been a mainstay of biotechnology research, allowing scientists to track gene expression, understand intracellular processes and debug engineered genetic circuits.

But conventional reporting schemes that rely on fluorescence and other optical approaches come with practical limitations that could cast a shadow over the field’s future progress. Now, researchers at the University of Washington and Microsoft have created a “nanopore-tal” into what is happening inside these complex biological systems, allowing scientists to see reporter proteins in a whole new light.

The team introduced a new class of reporter proteins that can be directly read by a commercially available nanopore sensing device. The new system ― dubbed “Nanopore-addressable protein Tags Engineered as Reporters” or “NanoporeTERs” ― can detect multiple protein expression levels from bacterial and human cell cultures far beyond the capacity of existing techniques.

An August 12, 2021 University of Washington news release (also on EurekAlert but published August 24, 2021), which originated the news item, provides more detail (Note: Links have been removed),

“NanoporeTERs offer a new and richer lexicon for engineered cells to express themselves and shed new light on the factors they are designed to track. They can tell us a lot more about what is happening in their environment all at once,” said co-lead author Nicolas Cardozo, a doctoral student with the UW Molecular Engineering and Sciences Institute. “We’re essentially making it possible for these cells to ‘talk’ to computers about what’s happening in their surroundings at a new level of detail, scale and efficiency that will enable deeper analysis than what we could do before.”

For conventional labeling methods, researchers can track only a few optical reporter proteins, such as green fluorescent protein, simultaneously because of their overlapping spectral properties. For example, it’s difficult to distinguish between more than three different colors of fluorescent proteins at once. In contrast, NanoporeTERs were designed to carry distinct protein “barcodes” composed of strings of amino acids that, when used in combination, allow at least ten times more multiplexing possibilities. 

These synthetic proteins are secreted outside of a cell into the surrounding environment, where researchers can collect and analyze them using a commercially available nanopore array. Here, the team used the Oxford Nanopore Technologies MinION device. 

The researchers engineered the NanoporeTER proteins with charged “tails” so that they can be pulled into the nanopore sensors by an electric field. Then the team uses machine learning to classify the electrical signals for each NanoporeTER barcode in order to determine each protein’s output levels.

“This is a fundamentally new interface between cells and computers,” said senior author Jeff Nivala, a UW research assistant professor in the Paul G. Allen School of Computer Science & Engineering. “One analogy I like to make is that fluorescent protein reporters are like lighthouses, and NanoporeTERs are like messages in a bottle. 

“Lighthouses are really useful for communicating a physical location, as you can literally see where the signal is coming from, but it’s hard to pack more information into that kind of signal. A message in a bottle, on the other hand, can pack a lot of information into a very small vessel, and you can send many of them off to another location to be read. You might lose sight of the precise physical location where the messages were sent, but for many applications that’s not going to be an issue.”

As a proof of concept, the team developed a library of more than 20 distinct NanoporeTERs tags. But the potential is significantly greater, according to co-lead author Karen Zhang, now a doctoral student in the UC Berkeley-UCSF bioengineering graduate program.

“We are currently working to scale up the number of NanoporeTERs to hundreds, thousands, maybe even millions more,” said Zhang, who graduated this year from the UW with bachelor’s degrees in both biochemistry and microbiology. “The more we have, the more things we can track.

“We’re particularly excited about the potential in single-cell proteomics, but this could also be a game-changer in terms of our ability to do multiplexed biosensing to diagnose disease and even target therapeutics to specific areas inside the body. And debugging complicated genetic circuit designs would become a whole lot easier and much less time-consuming if we could measure the performance of all the components in parallel instead of by trial and error.”

These researchers have made novel use of the MinION device before, when they developed a molecular tagging system to replace conventional inventory control methods. That system relied on barcodes comprising synthetic strands of DNA that could be decoded on demand using the portable reader. 

This time, the team went a step farther.

“This is the first paper to show how a commercial nanopore sensor device can be repurposed for applications other than the DNA and RNA sequencing for which they were originally designed,” said co-author Kathryn Doroschak, a computational biologist at Adaptive Biotechnologies who completed this work as a doctoral student at the Allen School. “This is exciting as a precursor for nanopore technology becoming more accessible and ubiquitous in the future. You can already plug a nanopore device into your cell phone. I could envision someday having a choice of ‘molecular apps’ that will be relatively inexpensive and widely available outside of traditional genomics.”

Additional co-authors of the paper are Aerilynn Nguyen at Northeastern University and Zoheb Siddiqui at Amazon, both former UW undergraduate students; Nicholas Bogard at Patch Biosciences, a former UW postdoctoral research associate; Luis Ceze, an Allen School professor; and Karin Strauss, an Allen School affiliate professor and a senior principal research manager at Microsoft. This research was funded by the National Science Foundation, the National Institutes of Health and a sponsored research agreement from Oxford Nanopore Technologies. 

Here’s a link to and a citation for the paper,

Multiplexed direct detection of barcoded protein reporters on a nanopore array by Nicolas Cardozo, Karen Zhang, Kathryn Doroschak, Aerilynn Nguyen, Zoheb Siddiqui, Nicholas Bogard, Karin Strauss, Luis Ceze & Jeff Nivala. Nature Biotechnology (2021) DOI: https://doi.org/10.1038/s41587-021-01002-6 Published: 12 August 2021

This paper is behind a paywall.

The how and why of nanopores

An August 19, 2021 Universidade NOVA de Lisboa ITQB NOVA press release (also on EurekAlert) explains what nanopores are while describing research into determining how their locations can be controlled,

At the simplest of levels, nanopores are (nanometre-sized) holes in an insulating membrane. The hole allows ions to pass through the membrane when a voltage is applied, resulting in a measurable current. When a molecule passes through a nanopore it causes a change in the current, this can be used to characterize and even identify individual molecules. Nanopores are extremely powerful single-molecule biosensing devices and can be used to detect and sequence DNA, RNA, and even proteins. Recently, it has been used in the SARS-CoV-2 virus sequencing.  

Solid-state nanopores are an extremely versatile type of nanopore formed in ultrathin membranes (less than 50 nanometres), made from materials such as silicon nitride (SiNx). Solid-state nanopores can be created with a range of diameters and can withstand a multitude of conditions (discover more about solid-state nanopore fabrication techniques here). One of the most appealing techniques with which to fabricate nanopores is Controlled Breakdown (CBD). This technique is quick, reduces fabrication costs, does not require specialized equipment, and can be automated.

CBD is a technique in which an electric field is applied across the membrane to induce a current. At some point, a spike in the current is observed, signifying pore formation. The voltage is then quickly reduced to ensure the fabrication of a single, small nanopore.

The mechanisms underlying this process have not been fully elucidated thus an international team involving ITQB NOVA decided to further investigate how electrical conduction through the membrane occurs during breakdown, namely how oxidation and reduction reactions (also called redox reactions, they imply electron loss or gain, respectively) influence the process. To do this, the team created three devices in which the electric field is applied to the membrane (a silicon-rich SiNx membrane) in different ways: via metal electrodes on both sides of the membrane; via electrolyte solutions on both sides of the membrane; and via a mixed device with a metal electrode on one side and an electrolyte solution on the other.

Results showed that redox reactions must occur at the membrane-electrolyte interface, whilst the metal electrodes circumvent this need. The team also demonstrated that, because of this phenomenon, nanopore fabrication could be localized to certain regions by performing CBD with metal microelectrodes on the membrane surface. Finally, by varying the content of silicon in the membrane, the investigators demonstrated that conduction and nanopore formation is highly dependent on the membrane material since it limits the electrical current in the membrane.

“Controlling the location of nanopores has been of interest to us for a number of years”, says James Yates. Pedro Sousa adds that “our findings suggest that CBD can be used to integrate pores with complementary micro or nanostructures, such as tunneling electrodes or field-effect sensors, across a range of different membrane materials.”  These devices may then be used for the detection of specific molecules, such as proteins, DNA, or antibodies, and applied to a wide array of scenarios, including pandemic surveillance or food safety.

This project was developed by a research team led by ITQB NOVA’s James Yates and has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement No 724300 and 875525). Co-author Pedro Miguel Sousa is also from ITQB NOVA. The other consortium members are from the University of Oxford, Oak Ridge National Laboratory, Imperial College London and Queen Mary University of London. The authors would like to thank Andrew Briggs for providing financial support.

Here’s a link to and a citation for the paper,

Understanding Electrical Conduction and Nanopore Formation During Controlled Breakdown by Jasper P. Fried, Jacob L. Swett, Binoy Paulose Nadappuram, Aleksandra Fedosyuk, Pedro Miguel Sousa, Dayrl P. Briggs, Aleksandar P. Ivanov, Joshua B. Edel, Jan A. Mol, James R. Yates. Small DOI: https://doi.org/10.1002/smll.202102543 First published: 01 August 2021

This paper is open access.

Iran nanotechnology sector update

It’s been a long time but last August I stumbled across a number of stories about Iran’s nanotechnology efforts.

First up, there’s an August 29, 2021 news item in Tehran Times,

As of the start of a national plan to develop the nanotechnology sector 15 years ago, more than 5,283 billion rials (about $125.7 million [US?] at the official exchange rate of 42,000 rials) has been allocated to nanotechnology projects, IRNA [Islamic Republic News Agency] reported on Saturday [August 28, 2021].

Nanotechnology’s trend of development is growing in Iran, as the number of nanoproducts and equipment developed in the previous [Iranian calendar] year (ended March 20 [2021]) increased to 750, compared with 647 a year before.

Some 223 product manufacturing companies and 59 equipment manufacturing companies are active in the field of nanotechnology and by the end of last year, which developed a total of 750 products and equipment.

Of the 750 products and equipment registered in the nanotechnology product database, 535 were related to nano-products and 215 were related to nano-equipment, both of which have experienced a growing trend over the past few years, although nano-products have grown more significantly.

Saeed Sarkar, the head of Iran’s Nanotechnology Innovation Council, said in June that Iran has created centers in six Asian countries for exporting nanotechnology products.

China, India, Indonesia, Syria, Turkey, and Iraq have received Iranian nanotechnology products and services.

Iranian-made nanotechnology products are currently exported to 49 countries in five continents, he added.

Iran has been introduced as the 4th leading country in the world in the field of nanotechnology, publishing 11,546 scientific articles in 2020.

The country held a 6 percent share of the world’s total nanotechnology articles, according to StatNano’s monthly evaluation accomplished in WoS [Web of Science] databases.

Then a national contest was announced in a September 1, 2021 news item in the Tehran Times, Note: Some of the information in this news item has been repeated from the August 31, 2021 news item],

The second round of the 10th National Nanotech Contest will be held on Friday [September 3, 2021] with 308 university students competing in 21 centers nationwide.

As the most significant academic event in the country, the national contest is held annually in the five fields of basic concepts, synthesis, analysis, applications, and commercialization of domestically-made products, ISNA reported.

The first round of the event was held July 31-August 2 [2021].

Finally, there’s a September 5, 2021 article by Faranak Bakhtiari about nanotechnology and drought abatement measures for the Tehran Times,

Iran is located in an arid and semi-arid region, and Iranians have long sought to make the most of water.

In recent years, the drought has intensified making water resources fragile and it can be said that we have reached water bankruptcy in Iran.

However, water stress will continue this fall (September 23-December 21 [2021]), and the season is expected to be relatively hot and short of rain, according to Ahad Vazifeh, head of the national center for drought and crisis management.

In such a situation, officials and experts propose various solutions for optimal water management.

Alireza Qazizadeh, a water and environment expert, referring to 80 percent of the arid regions in the country, said that “Iran has one percent of the earth’s area and receives only 36 percent of renewable resources.

The country receives 250 mm of rainfall annually, which is about 400 billion cubic meters, considering 70 percent evaporation, there is only 130 billion cubic meters of renewable water and 13 billion cubic meters of input from border waters.”

Referring to 800 ml of average rainfall and 700 mm of global evaporation, he noted that 70 percent of rainfall in Iran occurs in only 25 percent of the country and only 25 percent rains in irrigation seasons.

Pointing to the need for 113 billion cubic meters of water in the current year (began on March 21), he stated that “of this amount, 102 billion is projected for agricultural use, 7 percent for drinking and 2 percent for industry, and at this point water stress occurs.

In 2001, 5.5 billion cubic meters of underground resources were withdrawn annually, and if we consider this amount as 20 years from that year until now, it means that we have withdrawn an equivalent of one year of water consumption from non-renewable resources, which is alarming.”

Rasoul Sarraf, the Faculty of Materials at Shahid Modarres University, suggests a different solution and states that “To solve ease water stress, we have no choice but to use nanotechnology and solar power plants.

A recent report by Nature Scientific Journal on Iran’s water crisis indicates that from 2002 to 2015, over 74 billion cubic meters have been extracted from aquifers, which is unprecedented and its revival takes thousands of years along with urgent action.

Bakhtiari’s article does not mention how nanotechnology can be a factor in mitigating water shortages, rather it focuses on the urgency of the situation.

For anyone who thinks that droughts and water shortages do not affect Canada, take a good look at the Canadian Drought Monitor map (as of July 31, 2021). In an area known internationally for its rainfall (Vancouver and Vancouver Island in British Columbia), we had drought conditions ranging from severe to extreme. As for the rest of Canada, the prairie provinces seemed to have experienced the driest conditions with Manitoba experiencing some of the most extreme conditions in the country. From all reports, this will not be unusual in the future.

Salmon science camps

This story led me to a much larger international story about funding, which is usually not an exciting topic but this time, it was different.

First, there are the Salmon Science Camps.

A January 25, 2022 University of British Columbia (UBC) news release (also on EurekAlert and received via email) announces new funding for a STEM (science, technology, engineering, and mathematics) education initiative that focuses on Indigenous youth, salmon, and science,

Imagine a summer camp where you can watch grizzly bears catch salmon in streams, while learning about the migration and preparation of the fish hovering in the water at your feet.

Welcome to the Salmon Science Camp for Nisga’a youth, run by Dr. Andrea Reid (she/her), principal investigator of the Centre for Indigenous Fisheries at UBC. With new funding from the multi-institutional $24 million Ărramăt Project, Dr. Reid plans to expand these camps and open doors to scientific learning.

What are the Salmon Science camps?

We started these camps in 2016, with funding from the Gingolx Village Government Education Department and NSERC [Natural Sciences and Engineering Research Council of Canada] Indigenous Science Ambassadors Program, focusing on Nisga’a Nation youth aged four to 17 years old in Gingolx, my grandmother’s home village in British Columbia, at the base of the Alaska Panhandle. Each summer since, we charter boats and hire buses to get young people out onto the land and water where they follow the salmon life cycle, through all parts of the watershed from spawning grounds to the ocean and back again.

They learn to identify plants and animals, meet technicians working for the Nisga’a fisheries and wildlife department, learn from Elders who carry important stories about hoon (salmon) and how we care for them, and get to play and experiment with different scientific tools, from radio telemetry technology to underwater drones to water testing toolkits!

The Gingolx Village Government education manager Renee Garner said youth return from a day on the water feeling connected to one another. One student told her they had learned how the spirit bear got its name: fish cannot see their paws in the water, making them like ghosts and great hunters, something she would never forget.

What will the Ărramăt Project allow you to do?

Led by the University of Alberta, the Ărramăt Project is focused on strengthening human health and well-being through conservation and sustainable relationships with biodiversity. As one of 51 co-applicants from around the world on the recent New Frontiers in Research Fund Transformation grant awarded to this Indigenous-led project, my work will include expanding the camps to involve youth from the three other Nisga’a Nation villages: Gitlaxt’aamiks, Gitwinksihlkw, and Laxgalts’ap. We also want to create exchanges with neighbouring Nations, so camp attendees can learn about their different relationships with fish, including preparation methods and how they differ across cultures and environmental contexts. These exchanges will also promote cross-cultural learning and relationship building, bringing Indigenous youth together from across the province. All our activities build on the fundamental idea that salmon health and human well-being are inextricably linked, and we all need to do our part to ensure a better future for us all.

Why are these camps important?

These camps open a door to science and immersive learning experiences for Indigenous youth that might not necessarily be available due to the location of Gingolx, and they get to see a whole range of Nisga’a citizens as experts and scientists. This might mean they begin to see science as a future avenue for themselves, and view caring for salmon in the way Nisga’a have always done as not only an act of stewardship, but a truly scientific practice that is based on observation, experimentation, and other systematic ways of building knowledge about the world in which we all live. The camps demonstrate for youth that Indigenous science is science – it’s just as valid and important as conventional academic knowledge.

Interview language(s): English (Reid)

Congratulations to Dr. Reid!

Funding—have patience, it gets more interesting

Anyone who reads my postings with regularity will know I don’t often give compliments to funding agencies or the Canadian federal government for that matter. This time I have to offer kudos.

Breaking it down

As the news release notes, the salmon science camps got their start in 2016 with the Gingolx Village Government Education Department and the NSERC (Natural Sciences and Engineering Research Council of Canada) Indigenous Science Ambassadors Program.

(I found two different webpages for the Gingolx (Village Government) Education Department, this and this.)

NSERC has two programmes, the NSERC Student Ambassadors which was started in 2018 according to their webpage and the NSERC Indigenous Student Ambassadors, which does not include any history on its webpage.

It’s not clear as to whether the salmon science camps will continue getting the Gingolx/NSERC money now that a new agency and a new funding programme have become involved.

New agency

As noted in the news release, the Ărramăt Project (led by the University of Alberta) is funded under the New Frontiers in Research Fund, which itself was launched in 2018. From the About the New Frontiers in Research Fund webpage, Note: Links have been removed,

Launched in 2018, the New Frontiers in Research Fund (NFRF) funds interdisciplinary, high-risk / high-reward, transformative research led by Canadian researchers working with Canadian and international partners. The NFRF is designed to support world-leading innovation and enhance Canada’s competitiveness and expertise in the global, knowledge-based economy.

This fund seeks to inspire innovative research projects that push boundaries into exciting new areas and that have the potential to deliver game-changing impacts.

To meet its goals, the NFRF program is innovative in its design and implementation. Its novel merit review processes reflect the objectives of each funding opportunity, and the program offers flexibility in the use of grant funds to support international collaboration.

The NFRF is under the strategic direction of the Canada Research Coordinating Committee. It is administered by the Tri-agency Institutional Programs Secretariat, which is housed within the Social Sciences and Humanities Research Council (SSHRC), on behalf of Canada’s three federal research funding agencies: SSHRC, the Canadian Institutes of Health Research [CIHR] and the Natural Sciences and Engineering Research Council.

The NFRF has a budget of $275 million over five years (2018-19 to 2022-23), and will grow to have an annual budget of $124 million beginning in 2023-24.

The NFRF is split into four streams: Exploration, Transformation, International, and Special Calls. The Ărramăt Project has been funded as part of the Transformation stream. (For more about the Canada Research Coordinating Committee, the NFRF, and funding opportunities, go here, scroll down and you’ll see what you’re looking for on the right side of the screen.)

Fanfare: the Ărramăt Project

There’s a brief January 12, 2022 announcement on the Denakayeh website and here’s a PDF version of the announcement,

“There are very few places left on earth where nature and Indigenous Peoples
are not under stress. We urgently need solutions that can ensure health and well-being for future generations.” (Danika Billie Littlechild)

Biodiversity decline is a major issue in Canada and globally. Species extinctions, along with problems of land and water quality, are not just environmental issues. These losses are also leading to impacts on human health and well-being, particularly for Indigenous Peoples. As more and more lands, rivers, plants, and animals are lost and degraded, disease risks and food insecurity will become more common. Indigenous cultural practices, languages, and knowledges are threatened; however, they can also guide us towards necessary transformation.

“Conventional policy approaches don’t help us understand and address the linkages between environmental losses and human health problems like zoonotic diseases (e.g., COVID19). We have to get out of our disciplinary and bureaucratic silos and recognize that these ecological losses are interconnected to human health. They also cause economic and social stresses on families and communities.” (Brenda Parlee)

Ărramăt is a new project funded for 2021-2027 by the New Frontiers Research Fund Transformations Program (NFRF-T) in Canada, that is being launched in response to this global biodiversity and health crisis.

“The Ărramăt Project is about respecting the inherent dignity and interconnectedness of peoples and Mother Earth, life and livelihood, identity and expression, biodiversity and sustainability, and stewardship and well-being. Arramăt is a word from the Tamasheq language spoken by the Tuareg people of the Sahel and Sahara regions which reflects this holistic worldview.” (Mariam Wallet Aboubakrine)

Over 150 Indigenous organizations, universities, and other partners will work together to highlight the complex problems of biodiversity loss and its implications for health and well-being. The project Team will take a broad approach and be inclusive of many different worldviews and methods for research (i.e., intersectionality, interdisciplinary, transdisciplinary). Activities will occur in 70 different kinds of ecosystems that are also spiritually, culturally, and economically important to Indigenous Peoples.

The project is led by Indigenous scholars and activists Danika Billie Littlechild (Carleton University), Mariam Wallet Aboubakrine (former President of the United Nations Permanent Forum on Indigenous Issues), and Sherry Pictou (Dalhousie University). John O’Neil (former Dean of the Faculty of Health Sciences at Simon Fraser University) and Murray Humphries (Co-Director for the Centre for Indigenous Peoples’ Nutrition, and Environment at McGill University), are also Co- Principal Investigators of the project. The University of Alberta is the lead institution for the project (led by Brenda Parlee, Nominated Principal Investigator).

“The research builds on the momentum and opportunities created in Treaties, by the Truth and Reconciliation Commission (TRC), the National Inquiry into Missing and Murdered Indigenous Women, Girls and Two-Spirit People (MMIWG2S), and the United Nations Declaration on the Rights of Indigenous Peoples (UNDRIP). We want to harness that momentum in ways that can create fundamental change to the status quo around biodiversity and health.” (Sherry Pictou)

Over half of the $24 mil research budget will go directly to Indigenous governments and organizations to lead their own work in ways that respect, protect, and elevate the knowledges and Indigenous ways of life. Cultural security and social justice for women and those of the 2SLGBTQQIA+ and ancestral gender diverse communities, will be central to the work of this Team as they address fundamental questions of common concern. How can food security be strengthened for Indigenous Peoples? What are Indigenous-led approaches to conservation that support wild species and agrobiodiversity? What are the best practices for decolonizing education and science? How can we include the voices of Indigenous youth? How can we address the widespread and recurring violence against Mother Earth and Indigenous Peoples? Can we foster healthier relationships to nature? How can we emotionally and spiritually heal from the stresses and losses caused by colonial practices (e.g., residential schools), land and resource development, and climate change?

The diversity of Indigenous Peoples, knowledges, and interdisciplinary Team expertise will be mobilized through the project to produce action at local to global scales of decision-making. Dene, Nisga’a (Canada), and Batwa (Uganda) aim to produce new models of conservation for ‘species at risk’ [emphasis mine]. Other groups such as the peoples of Treaty 8 and Treaty 3 (Canada), Yawanawà (Brazil), and Aymara (Bolivia) will focus on improving land and water security. Alternative economic and livelihood strategies (e.g., Indigenous Guardians) that benefit people and nature will be a focus for Indigenous Peoples in regions such as northern Canada, the Sahara and Sahel regions, and Thailand. The knowledge and customary strategies of Māori (Aotearoa-New Zealand) will contribute to the reconnection communities to their land and seascapes and regeneration of their cultural-ecological systems. The knowledges of Nêhiyawak (Cree), Sámi, and Tribal Peoples of India will be a foundation for action to rewild or restore cultural values and uses of other degraded landscapes. More than 140 projects will be funded on these and other themes over the 6 years.

“It is an honour and a profound responsibility to be part of this Indigenous-led project. It is unique from many other large projects in its embrace of governance models like ethical space, Indigenous research methodologies, and Indigenous Knowledges.” (John O’Neil)

“I am excited to see the work reveal how Indigenous Knowledges and stewardship practices define both the origins and contemporary centres of ecological research, biodiversity science, and conservation biology.” (Murray Humphries)

By 2027, the project will have produced a diversity of holistic and actionable solutions for improved stewardship and care for people and the planet.

“Strategies for biodiversity conservation have not historically been positive for Indigenous Peoples. They have a very small voice, if any, at the tables of decision-making. We don’t just want to be token members of the colonial structures that currently exist, we want to decolonize and Indigenize decisions about nature and health. Everyone needs to be accountable. We will not give up on Mother Earth and the possibility of renewing, strengthening, and elevating the health and well-being of Indigenous Peoples, their lands and waters, and all beings who rely upon them.” (Danika Billie Littlechild)

The compliments and getting back to the salmon science camps

The Ărramăt Project’s scope is breathtaking and necessary. Bravo!

I want to recognize the funding agencies (SSHRC, NSERC, and CIHR). Bravo!

Plus the Gingolx Village Government Education Department. Bravo!

And, I want to acknowledge one other group (from the Acknowledging New Frontiers in Research Fund Support and Communicating the Value of your Research webpage),

Federal support for research is an investment by the people of Canada [emphasis mine]. It is important for taxpayers to know how research dollars are being spent. By demonstrating the value of your research, New Frontiers in Research Fund (NFRF) award recipients help strengthen public understanding of and support for high-risk, high-reward, interdisciplinary and international research.

Finally, Brava Dr. Reid! I don’t imagine it was easy to start your project and keep it running.

Canadians and their government have a great deal to grapple with in regard to indigenous people and much of it quite ugly. This funding doesn’t negate the past or absolve anyone of their sins but it does point to new possibilities for our relationships with each other and with our planet. (For anyone unfamiliar with the history of the relationship between the Canadian government and its Indigenous peoples there’s this essay on Wikipedia. Also, here’s the Residential Schools in Canada essay in the Canadian Encyclopedia and and there’s more here on the federal government’s Residential schools in Canada webpage.)

Not to get too carried away with grand visions, here’s a science salmon camp video,

Small steps, eh?