A July 27, 2020 news item on Nanowerk announces research into gut health described as seminal (Note: A link has been removed),
An international team of scientists has completed the first ever study into the potential impact of naturally occurring and man-made nanoparticles on the health of all types of the major living species of animals.
Conceived by researchers at the University of Plymouth, as part of the EU [European Union] Nanofase project, the study assessed how the guts of species from honey bees to humans could protect against the bioaccumulation and toxicological effects of engineered nanomaterials (ENMs) found within the environment.
It showed that the digestive systems of many species have evolved to act as a barrier guarding against the absorption of potentially damaging particles.
However, invertebrates such as earthworms also have roving cells within their guts, which can take up ENMs and transfer them to the gut wall.
This represents an additional risk for many invertebrate species where the particles can be absorbed via these roving cells, with consequent effects on internal organs having the potential to cause lasting damage.
Fortunately, this process is not replicated in humans and other vertebrate animals, however there is still the potential for nanomaterials to have a negative impact through the food chain.
The study, published in the July  edition of Environmental Science: Nano, involved scientists from the UK, the Netherlands, Slovenia and Portugal and focused on particles measuring up to 100 nanometres (around 1/10 millionth of a metre).
It combined existing and new research into species including insects and other invertebrates, fish, birds, and mammals, as well as identifying knowledge gaps on reptiles and amphibians. The study provides the first comprehensive overview of how differences in gut structure can affect the impact of ENMs across the animal kingdom.
Richard Handy, Professor of Environmental Toxicology at the University of Plymouth and the study’s senior author, said:
“This is a seminal piece work that combines nearly 100 years of zoology research with our current understanding of nanotechnology.
“The threats posed by engineered nanomaterials are becoming better known, but this study provides the first comprehensive and species-level assessment of how they might pose current and future threats. It should set the foundations for understanding the dietary hazard in the animal kingdom.”
Nanomaterials come in three forms – naturally occurring, incidentally occurring from human activities, and deliberately manufactured – and their use has increased exponentially in the last decade.
They have consistently found new applications in a wide variety of industrial sectors, including electrical appliances, medicines, cleaning products and textiles.
Professor Handy, who has advised organisations including the Organisation for Economic Co-operation and Development and the United States National Nanotechnology Initiative, added:
“Nanoparticles are far too small for the human eye to see but that doesn’t mean they cannot cause harm to living species. The review element of this study has shown they have actually been written about for many decades, but it is only recently that we have begun to understand the various ways they occur and now the extent to which they can be taken up. Our new EU project, NanoHarmony, looks to build on that knowledge and we are currently working with Public Health England and others to expand our method for detecting nanomaterials in tissues for food safety and other public health matters.”
More and more, this resembles a public relations campaign. First, CRISPR (clustered regularly interspersed short palindromic repeats) gene editing is going to be helpful with COVID-19 and now it can help us to deal with conservation issues. (See my May 26, 2020 posting about the latest CRISPR doings as of May 7, 2020; included is a brief description of the patent dispute between Broad Institute and UC Berkeley and musings about a public relations campaign.)
The gene-editing technology CRISPR has been used for a variety of agricultural and public health purposes — from growing disease-resistant crops to, more recently, a diagnostic test for the virus that causes COVID-19. Now a study involving fish that look nearly identical to the endangered Delta smelt finds that CRISPR can be a conservation and resource management tool, as well. The researchers think its ability to rapidly detect and differentiate among species could revolutionize environmental monitoring.
The study, published in the journal Molecular Ecology Resources, was led by scientists at the University of California, Davis, and the California Department of Water Resources in collaboration with MIT Broad Institute [emphasis mine].
As a proof of concept, it found that the CRISPR-based detection platform SHERLOCK (Specific High-sensitivity Enzymatic Reporter Unlocking) [emphasis mine] was able to genetically distinguish threatened fish species from similar-looking nonnative species in nearly real time, with no need to extract DNA.
“CRISPR can do a lot more than edit genomes,” said co-author Andrea Schreier, an adjunct assistant professor in the UC Davis animal science department. “It can be used for some really cool ecological applications, and we’re just now exploring that.”
WHEN GETTING IT WRONG IS A BIG DEAL
The scientists focused on three fish species of management concern in the San Francisco Estuary: the U.S. threatened and California endangered Delta smelt, the California threatened longfin smelt and the nonnative wakasagi. These three species are notoriously difficult to visually identify, particularly in their younger stages.
Hundreds of thousands of Delta smelt once lived in the Sacramento-San Joaquin Delta before the population crashed in the 1980s. Only a few thousand are estimated to remain in the wild.
“When you’re trying to identify an endangered species, getting it wrong is a big deal,” said lead author Melinda Baerwald, a project scientist at UC Davis at the time the study was conceived and currently an environmental program manager with California Department of Water Resources.
For example, state and federal water pumping projects have to reduce water exports if enough endangered species, like Delta smelt or winter-run chinook salmon, get sucked into the pumps. Rapid identification makes real-time decision making about water operations feasible.
FROM HOURS TO MINUTES
Typically to accurately identify the species, researchers rub a swab over the fish to collect a mucus sample or take a fin clip for a tissue sample. Then they drive or ship it to a lab for a genetic identification test and await the results. Not counting travel time, that can take, at best, about four hours.
SHERLOCK shortens this process from hours to minutes. Researchers can identify the species within about 20 minutes, at remote locations, noninvasively, with no specialized lab equipment. Instead, they use either a handheld fluorescence reader or a flow strip that works much like a pregnancy test — a band on the strip shows if the target species is present.
“Anyone working anywhere could use this tool to quickly come up with a species identification,” Schreier said.
OTHER CRYPTIC CRITTERS
While the three fish species were the only animals tested for this study, the researchers expect the method could be used for other species, though more research is needed to confirm. If so, this sort of onsite, real-time capability may be useful for confirming species at crime scenes, in the animal trade at border crossings, for monitoring poaching, and for other animal and human health applications.
“There are a lot of cryptic species we can’t accurately identify with our naked eye,” Baerwald said. “Our partners at MIT are really interested in pathogen detection for humans. We’re interested in pathogen detection for animals as well as using the tool for other conservation issues.”
SHERLOCK is an evolution of CRISPR technology, which others use to make precise edits in genetic code. SHERLOCK can detect the unique genetic fingerprints of virtually any DNA or RNA sequence in any organism or pathogen. Developed by our founders and licensed exclusively from the Broad Institute, SHERLOCK is a method for single molecule detection of nucleic acid targets and stands for Specific High Sensitivity Enzymatic Reporter unLOCKing. It works by amplifying genetic sequences and programming a CRISPR molecule to detect the presence of a specific genetic signature in a sample, which can also be quantified. When it finds those signatures, the CRISPR enzyme is activated and releases a robust signal. This signal can be adapted to work on a simple paper strip test, in laboratory equipment, or to provide an electrochemical readout that can be read with a mobile phone.
Ensuring the SHERLOCK diagnostic platform is easily accessible, especially in the developing world, where the need for inexpensive, reliable, field-based diagnostics is the most urgent
SHERLOCK (Specific High-sensitivity Enzymatic Reporter unLOCKing) is a CRISPR-based diagnostic tool that is rapid, inexpensive, and highly sensitive, with the potential to have a transformative effect on research and global public health. The SHERLOCK platform can detect viruses, bacteria, or other targets in clinical samples such as urine or blood, and reveal results on a paper strip — without the need for extensive specialized equipment. This technology could potentially be used to aid the response to infectious disease outbreaks, monitor antibiotic resistance, detect cancer, and more. SHERLOCK tools are freely available [emphasis mine] for academic research worldwide, and the Broad Institute’s licensing framework [emphasis mine] ensures that the SHERLOCK diagnostic platform is easily accessible in the developing world, where inexpensive, reliable, field-based diagnostics are urgently needed.
Here’s what I suspect. as stated, the Broad Institute has free SHERLOCK licenses for academic institutions and not-for-profit organizations but Sherlock Biosciences, a Broad Institute spinoff company, is for-profit and has trademarked SHERLOCK for commercial purposes.
This looks like a relatively subtle campaign to influence public perceptions. Genetic modification or genetic engineering as exemplified by the CRISPR gene editing technique is a force for the good of all. It will help us in our hour of need (COVID-19 pandemic) and it can help us save various species and better manage our resources.
This contrasts greatly with the publicity generated by the CRISPR twins situation where a scientist claimed to have successfully edited the germline for twins, Lulu and Nana. This was done despite a voluntary, worldwide moratorium on germline editing of viable embryos. (Search the terms [either here or on a standard search engine] ‘CRISPR twins’, ‘Lulu and Nana’, and/or ‘He Jiankui’ for details about the scandal.
In addition to presenting CRISPR as beneficial in the short term rather than the distant future, this publicity also subtly positions the Broad Institute as CRISPR’s owner.
Nanofibre magic turns fish skins into wrinkle busting skin care
Sanford partners with kiwi nanotech experts to help develop a wrinkle-busting skincare product made from Hoki skins.
New Zealand’s biggest and oldest seafood company is moving into the future of skincare and medicine by becoming supporting partner to West Auckland nanofibre producer Revolution Fibres, which is launching a potentially game-changing nanotech face mask.
The actiVLayr face masks use collagen extracted from fish skins as a base ingredient which is then combined with elements such as fruit extracts and hyaluronic acid to make a 100 percent natural and sustainably sourced product.
They have achieved stunning results in third party tests which show that the nanofiber masks can reduce wrinkles by up to 31.5%.*
Revolution Fibres CEO Iain Hosie says it is no exaggeration to say the masks could be revolutionary.
“The wayactiVLayr is produced, and the unique application method of placing it onto wet skin like a mask, means ingredients are absorbed quickly and efficiently into the skin to maximise the repair and protection of the skin.”
Sanford is delighted to support the work that Revolution Fibres is doing by supplying hoki fish skins. Hoki is a sustainably caught fish and its skin has some unique properties.
Sanford’s General Manager of Innovation, Andrew Stanley, says these properties make it ideal for the actiVLayr technology. “Hoki skins are rich in collagen, which is an essential part of our bodies. But their marine collagen is unique – it has a very low melt point, so when placed on the skin, it can dissolve completely and be absorbed in a way that collagen f rom other animals cannot.”
Sanford’s Chief Customer Officer, Andre Gargiulo, says working with the team at Revolution Fibres is a natural fit, because both company’s think about innovation and sustainability in the same way.
“We hope actiVLayr gets the global attention it deserves, and we’re delighted that our sustainably caught Hoki is part of this fantastic New Zealand product. It’s exactly what we’re all about at Sanford – making the most of the precious resources from the sea, working in a sustainable way and getting the most value out of the goodness we harvest from nature.”
Sanford’s Business Development Manager Adrian Grey says the focus on sustainability and value creation are so important for the seafood company.
“Previously we have been making use of these hoki skins, which is great, but they were being used only for fish meal or pet food products. Being able to supply and support a high tech company that is going to earn increased export revenue for New Zealand is just fantastic. And the product created is completely natural, harvested from a globally certified sustainable fishery.”
Sanford provides the hoki skins and then turns these skins into pure collagen using the science and skills of the team at Plant and Food in Nelson [New Zealand for those of us who associate Nelson with British Columbia]. Revolution Fibres transforms the Sanford product into nanofibre using a technique called electrospinning of which Revolution Fibres are the New Zealand pioneers.
During the electrospinning process natural ingredients known as “bioactives” (such as kiwifruit and grapes) and hyaluronic acid (an ingredient to help the skin retain moisture) are bonded to the nanofibres to create sheets of actiVLayr. When it is exposed to wet skin the nanofibres dissolve rapidly and release the bioactives deep into the skin.
The product is being launched at the China Beauty Fair in Shanghai on May 22  and will go on sale in China this month followed by Hong Kong and New Zealand later in the year. Revolution Fibres CEO Iain Hosie says there is big demand for unique delivery systems of natural skin and beauty products such as actiVLayr in Asia, which was the key reason to launch the product in China. But his view of the future is even bigger.
“There are endless uses for actiVLayr and the one we’re most proud of is in the medical area with the ability for drug compounds or medicines to be added to the actiVLayr formula. It will enable a controlled dose to be delivered to a patient with skin lesions, burns or acne.”
Revolution Fibres is presenting at Techweek NZ as part of The Fourth Revolution event on May 25  in Christchurch which introduces high tech engineers who are building a better place.
*Testing conducted by Easy Care using VISIA Complexion Analysis
The media advisory also includes some ‘fascinating ‘facts’,
1kg of hoki skin produces 400 square meters of nanofibre material
Nanofibres are 1/500th the width of a human hair
Revolution Fibres is the only nanofibre producer in the world to meet aerospace industry standards with its AS9100d quality assurance certification
The marine collagen found in hoki skins is unique because of its relatively low melt point, meaning it can dissolve at a lower temperature which makes it perfect for human use
Revolution Fibres is based in West Auckland and employs 12 people, of which 4 have P hDs in science related to nanotechnology. There are also a number of employees with strong engineering backgrounds to complement the company’s Research & Development expertise
Sanford is New Zealand’s oldest and biggest seafood company. It was founded by Albert Sanford in Auckland in 1904
New Zealand’s hoki fishery is certified as sustainable by the London-based Marine Stewardship Council, which audits fisheries all over the world
For some perspective on the business side of things, there’s a May 21, 2018 article by Nikki Mandow for newsroom.co.nz,
Revolution Fibres first started talking about the possibility of a collagen nanofibre made from hoki almost a decade ago, as part of a project with Plant & Food’s Seafood Research Centre in Nelson, Hosie [Revolution Fibres CEO Iain Hosie] said, and the company got serious about making a product in 2013.
Previously, the hoki waste skins were used for fish meal and pet food, said Sanford business development manager Adrian Grey.
“Being able to supply and support a high tech company that is going to earn increased export revenue for New Zealand is just fantastic.”
Revolution Fibres also manufactures nanofibres for a number of other uses. These include anti-dust mite pillow coverings, anti-pollution protective face masks, filters for pumps for HRV’s home ventilation systems, and reinforcing material for carbon fibre for fishing rods. The latter product is made from recycled fishing nets collected from South America.
He [Revolution Fibres CEO Iain Hosie] said the company could be profitable, but instead has chosen to continue to invest heavily in research and development.
About 75 percent of revenue comes from selling proprietary products, but increasingly Hosie said the company is working on “co-innovation” projects, where Revolution Fibres manufactures bespoke materials for outside companies.
Revolution Fibres completed its first external funding round last year, raising $1.5 million from the US, and it has just completed another round worth approximately $1million. Hosie, one of the founders, still holds around 20 percent of the company.
He said he hopes to keep the intellectual property in New Zealand, although manufacturing of some products is likely to move closer to their markets – China and the US potentially. However, he said actiVLayr manufacture will remain in New Zealand, because that’s where the raw hoki comes from.
I wonder if we’ll see this product in Canada.
One other thing, I was curious about this ” … the nanofiber masks can reduce wrinkles by up to 31.5%” and Visia Complexion Analysis, which is a product from Canfield Scientific, a company specializing in imaging. Here’s some of what Visia can do (from the Visia product page),
VISIA’s patented comparison to norms analysis uses the world’s largest skin feature database to grade your patient’s skin relative to others of the same age and skin type. Measure spots, wrinkles, texture, pores, UV spots, brown spots, red areas, and porphyrins.
Compare results side by side for any combination of views, features or time points, including graphs and numerical data. Zoom and pan images in tandem for clear and easy comparisons.
And, there’s my personal favourite (although it has nothing to do with the topic of this posting0,
Evaluates the results of lash improvement treatments with numerical assessments and graphic visualizations.
For anyone who wondered about why the press release has both ‘nanofibre’ and ‘nanofiber’, It’s the difference between US and UK spelling. Perhaps the complexion analysis information came from a US company or one that uses US spellings.
Cryopreservation and the promise of animal revivification is not one of my favourite topics, from a July 13, 2017 news item on Nanowerk (Note: A link has been removed),
Scientists report for the first time the ability to both deep freeze and reanimate zebrafish embryos. The method, appearing in the journal ACS Nano (“Gold Nanorod Induced Warming of Embryos from the Cryogenic State Enhances Viability”), could potentially be used to bank larger aquatic and other vertebrate oocytes and embryos, too, for a life in the future.
Cryopreservation has been used to save sperm, oocytes and even embryos of many species, including humans, cattle and lab animals. Preserving the embryos of most fishes, however, has remained an elusive goal. The embryos are relatively large with big yolks and are divided by multiple compartments. These traits make the embryos difficult to cool and warm uniformly without damage and ice formation. A few techniques, including microinjection of cryoprotectants and laser irradiation for re-warming, have shown promise toward achieving this long-sought goal. John Bischof and colleagues wanted to tweak the methods to see if they could finally make cryopreserving fish a reality.
The researchers injected a cryoprotectant, along with plasmonic gold nanoparticles to serve as a laser absorber, directly into zebrafish embryos. Plunging the embryos in liquid nitrogen rapidly cooled them to a cryogenically stable state in less than a second, according to modeling results. The researchers then used laser irradiation to heat up the nanoparticles, which were uniformly distributed inside the embryos, at an ultra-fast rate (1.4 x 107 degrees Celsius per minute). Not all of the embryos made it, but many were revived –a feat that is currently not possible by other techniques. Their hearts, eyes and nervous systems developed through at least the next 28 hours — and they started to wiggle. As more fish populations shrink and become threatened, the researchers say the cryopreservation method could help establish banks of frozen fish germ cells and embryos that could one day help replenish the oceans’ biodiversity. The technique could also be applied to amphibian, reptile and bird species with similar embryonic sizes and structures.
Here’s a video describing the work,
After watching that a video, I feel that I should revise my opinion of cryopreservation,
Preserving a smell? It’s an intriguing idea and forms the research focus for scientists at the University College London’s (UCL) Institute for Sustainable Heritage according to an April 6, 2017 Biomed Central news release on EurekAlert,
A ‘Historic Book Odour Wheel’ which has been developed to document and archive the aroma associated with old books, is being presented in a study in the open access journal Heritage Science. Researchers at UCL Institute for Sustainable Heritage created the wheel as part of an experiment in which they asked visitors to St Paul’s Cathedral’s Dean and Chapter library in London to characterize its smell.
The visitors most frequently described the aroma of the library as ‘woody’ (selected by 100% of the visitors who were asked), followed by ‘smoky’ (86%), ‘earthy'(71%) and ‘vanilla’ (41%). The intensity of the smells was assessed as between ‘strong odor’ and ‘very strong odor’. Over 70% of the visitors described the smell as pleasant, 14% as ‘mildly pleasant’ and 14% as ‘neutral’.
In a separate experiment, the researchers presented visitors to the Birmingham Museum and Art Gallery with an unlabelled historic book smell – sampled from a 1928 book they obtained from a second-hand bookshop in London – and collected the terms used to describe the smell. The word ‘chocolate’ – or variations such as ‘cocoa’ or ‘chocolatey’ – was used most often, followed by ‘coffee’, ‘old’, ‘wood’ and ‘burnt’. Participants also mentioned smells including ‘fish’, ‘body odour’, ‘rotten socks’ and ‘mothballs’.
Cecilia Bembibre, heritage scientist at UCL and corresponding author of the study said: “Our odour wheel provides an example of how scientists and historians could begin to identify, analyze and document smells that have cultural significance, such as the aroma of old books in historic libraries. The role of smells in how we perceive heritage has not been systematically explored until now.”
Attempting to answer the question of whether certain smells could be considered part of our cultural heritage and if so how they could be identified, protected and conserved, the researchers also conducted a chemical analysis of volatile organic compounds (VOCs) which they sampled from books in the library. VOCs are chemicals that evaporate at low temperatures, many of which can be perceived as scents or odors.
Combining their findings from the VOC analysis with the visitors’ characterizations, the authors created their Historic Book Odour wheel, which shows the chemical description of a smell (such as acetic acid) together with the sensory descriptions provided by the visitors (such as ‘vinegar’).
Cecilia Bembibre said: “By documenting the words used by the visitors to describe a heritage smell, our study opens a discussion about developing a vocabulary to identify aromas that have cultural meaning and significance.”
She added: “The Historic Book Odour Wheel also has the potential to be used as a diagnostic tool by conservators, informing on the condition of an object, for example its state of decay, through its olfactory profile.”
The authors suggest that, in addition to its use for the identification and conservation of smells, the Historic Book Odour Wheel could potentially be used to recreate smells and aid the design of olfactory experiences in museums, allowing visitors to form a personal connection with exhibits by allowing them to understand what the past smelled like.
Before this can be done, further research is needed to build on the preliminary findings in this study to allow them to inform and benefit heritage management, conservation, visitor experience design and heritage policy making.
Here’s what the Historic Book Odour Wheel looks like,
Odour wheel of historic book containing general aroma categories, sensory descriptors and chemical information on the smells as sampled (colours are arbitrary) Courtesy: Heritage Science [downloaded from https://heritagesciencejournal.springeropen.com/articles/10.1186/s40494-016-0114-1
I’ve heard of Lake Como in Italy but Como Lake in Minnesota is a new one for me. The Minnesota lake is featured in a March 22, 2017 news item about water and sponges on phys.org,
Mercury is very toxic and can cause long-term health damage, but removing it from water is challenging. To address this growing problem, University of Minnesota College of Food, Agricultural and Natural Sciences (CFANS) Professor Abdennour Abbas and his lab team created a sponge that can absorb mercury from a polluted water source within seconds. Thanks to the application of nanotechnology, the team developed a sponge with outstanding mercury adsorption properties where mercury contaminations can be removed from tap, lake and industrial wastewater to below detectable limits in less than 5 seconds (or around 5 minutes for industrial wastewater). The sponge converts the contamination into a non-toxic complex so it can be disposed of in a landfill after use. The sponge also kills bacterial and fungal microbes.
Think of it this way: If Como Lake in St. Paul was contaminated with mercury at the EPA limit, the sponge needed to remove all of the mercury would be the size of a basketball.
This is an important advancement for the state of Minnesota, as more than two thirds of the waters on Minnesota’s 2004 Impaired Waters List are impaired because of mercury contamination that ranges from 0.27 to 12.43 ng/L (the EPA limit is 2 ng/L). Mercury contamination of lake waters results in mercury accumulation in fish, leading the Minnesota Department of Health to establish fish consumption guidelines. A number of fish species store-bought or caught in Minnesota lakes are not advised for consumption more than once a week or even once a month. In Minnesota’s North Shore, 10 percent of tested newborns had mercury concentrations above the EPA reference dose for methylmercury (the form of mercury found in fish). This means that some pregnant women in the Lake Superior region, and in Minnesota, have mercury exposures that need to be reduced. In addition, a reduced deposition of mercury is projected to have economic benefits reflected by an annual state willingness-to-pay of $212 million in Minnesota alone.
In addition to improving air and water quality, aquatic life and public health, the new technology would have an impact on inspiring new regulations. Technology shapes regulations, which in turn determine the value of the market. The 2015 EPA Mercury and Air Toxics Standards regulation was estimated to cost the industry around of $9.6 billion annually in 2020. The new U of M technology has a potential of bringing this cost down and make it easy for the industry to meet regulatory requirements.
Research by Abbas and his team was funded by the MnDRIVE Global Food Venture, MnDRIVE Environment, and USDA-NIFA. They currently have three patents on this technology. To learn more, visit www.abbaslab.com.
Researchers in India have found a way to make use of fish ‘biowaste’ according to a Sept. 6, 2016 news item on Nanowerk,
Large quantities of fish are consumed in India on a daily basis, which generates a huge amount of fish “biowaste” materials. In an attempt to do something positive with this biowaste, a team of researchers at Jadavpur University in Koltata, India explored recycling the fish byproducts into an energy harvester for self-powered electronics.
Caption: Waste fish scales (upper left corner) are used to fabricate flexible nanogenerator (lower left) that power up more than 50 blue LEDs (lower right). An enlarged microscopic view of a fish scale shows the well-aligned collagen fibrils (upper right). The possibility of making a fish scale transparent (middle) and rollable (extreme left lower corner) is also illustrated. Credit: Sujoy Kuman Ghosh and Dipankar Mandal/Jadavpur University
The basic premise behind the researchers’ work is simple: Fish scales contain collagen fibers that possess a piezoelectric property, which means that an electric charge is generated in response to applying a mechanical stress. As the team reports this week in Applied Physics Letters, from AIP Publishing, they were able to harness this property to fabricate a bio-piezoelectric nanogenerator.
To do this, the researchers first “collected biowaste in the form of hard, raw fish scales from a fish processing market, and then used a demineralization process to make them transparent and flexible,” explained Dipankar Mandal, assistant professor, Organic Nano-Piezoelectric Device Laboratory, Department of Physics, at Jadavpur University.
The collagens within the processed fish scales serve as an active piezoelectric element.
“We were able to make a bio-piezoelectric nanogenerator — a.k.a. energy harvester — with electrodes on both sides, and then laminated it,” Mandal said.
While it’s well known that a single collagen nanofiber exhibits piezoelectricity, until now no one had attempted to focus on hierarchically organizing the collagen nanofibrils within the natural fish scales.
“We wanted to explore what happens to the piezoelectric yield when a bunch of collagen nanofibrils are hierarchically well aligned and self-assembled in the fish scales,” he added. “And we discovered that the piezoelectricity of the fish scale collagen is quite large (~5 pC/N), which we were able to confirm via direct measurement.”
Beyond that, the polarization-electric field hysteresis loop and resulting strain-electric field hysteresis loop — proof of a converse piezoelectric effect — caused by the “nonlinear” electrostriction effect backed up their findings.
The team’s work is the first known demonstration of the direct piezoelectric effect of fish scales from electricity generated by a bio-piezoelectric nanogenerator under mechanical stimuli — without the need for any post-electrical poling treatments.
“We’re well aware of the disadvantages of the post-processing treatments of piezoelectric materials,” Mandal noted.
To explore the fish scale collagen’s self-alignment phenomena, the researchers used near-edge X-ray absorption fine-structure spectroscopy, measured at the Raja Ramanna Centre for Advanced Technology in Indore, India.
Experimental and theoretical tests helped them clarify the energy scavenging performance of the bio-piezoelectric nanogenerator. It’s capable of scavenging several types of ambient mechanical energies — including body movements, machine and sound vibrations, and wind flow. Even repeatedly touching the bio-piezoelectric nanogenerator with a finger can turn on more than 50 blue LEDs.
“We expect our work to greatly impact the field of self-powered flexible electronics,” Mandal said. “To date, despite several extraordinary efforts, no one else has been able to make a biodegradable energy harvester in a cost-effective, single-step process.”
The group’s work could potentially be for use in transparent electronics, biocompatible and biodegradable electronics, edible electronics, self-powered implantable medical devices, surgeries, e-healthcare monitoring, as well as in vitro and in vivo diagnostics, apart from its myriad uses for portable electronics.
“In the future, our goal is to implant a bio-piezoelectric nanogenerator into a heart for pacemaker devices, where it will continuously generate power from heartbeats for the device’s operation,” Mandal said. “Then it will degrade when no longer needed. Since heart tissue is also composed of collagen, our bio-piezoelectric nanogenerator is expected to be very compatible with the heart.”
The group’s bio-piezoelectric nanogenerator may also help with targeted drug delivery, which is currently generating interest as a way of recovering in vivo cancer cells and also to stimulate different types of damaged tissues.
“So we expect our work to have enormous importance for next-generation implantable medical devices,” he added.
“Our end goal is to design and engineer sophisticated ingestible electronics composed of nontoxic materials that are useful for a wide range of diagnostic and therapeutic applications,” said Mandal.
I received a news release from a start-up company, New Wave Foods, which specializes in creating plant-based seafood. The concept looks very interesting and sci fi (Lois McMaster Bujold, and I’m sure others, has featured vat-grown meat and fish in her novels). Apparently, Google has already started using some of the New Wave product in its employee cafeteria. Here’s more from the July 19, 2016 New Wave Foods news release,
New Wave Foods announced today that it has successfully opened a seed round aimed at developing seafood that is healthier for humans and the planet. Efficient Capacity kicked off the round and New Crop Capital provided additional funding.
New Wave Foods uses plant-based ingredients, such as red algae, to engineer new edible materials that replicate the taste and texture of fish and shellfish while improving their nutritional profiles. Its first product, which has already been served in Google’s cafeterias, will be a truly sustainable shrimp. Shrimp is the nation’s most popular seafood, currently representing more than a quarter of the four billion pounds of fish and shellfish consumed by Americans annually. For each pound of shrimp caught, up to 15 pounds of other animals, including endangered dolphins, turtles, and sharks, die.
The market for meat analogs is expected to surpass $5 billion by 2020, and savvy investors are increasingly taking notice. In recent years, millions in venture capital has flowed into plant-based alternatives to animal foods from large food processors and investors like Bill Gates and Li Ka-shing, Asia’s richest businessman.
“The astounding scale of our consumption of sea animals is decimating ocean ecosystems through overfishing, massive death through bycatch, water pollution, carbon emissions, derelict fishing gear, mangrove deforestation, and more,” said New Wave Foods co-founder and CEO Dominique Barnes. “Shrimping is also fraught with human rights abuses and slave labor, so we’re pleased to introduce a product that is better for people, the planet, and animals.”
Efficient Capacity is an investment fund that advises and invests in companies worldwide. Efficient Capacity partners have founded or co-founded more than ten companies and served as advisors or directors to dozens of others.
New Crop Capital is a specialized private venture capital fund that provides early-stage investments to companies that develop “clean,” (i.e., cultured) and plant-based meat, dairy, and egg products or facilitate the promotion and sale of such products.
The current round of investments follows investments from SOS Ventures via IndieBio, an accelerator group funding and building biotech startups. IndieBio companies use technology to solve our culture’s most challenging problems, such as feeding a growing population sustainably. Along with investment, IndieBio offers its startups resources such as lab space and mentorship to help take an idea to a product.
Along with its funding round, New Wave Foods announced the appointment of John Wiest as COO. Wiest brings more than 15 years of senior management experience in food and consumer products, including animal-based seafood companies, to the company. As an executive and consultant, Wiest has helped dozens of food ventures develop new products, expand distribution channels, and create strategic partnerships.
New Wave Foods, founded in 2015, is a leader in plant-based seafood that is healthier and better for the environment. New Wave products are high in clean nutrients and deliver a culinary experience consumers expect without the devastating environmental impact of commercial fishing. Co-founder and CEO Dominique Barnes holds a master’s in marine biodiversity and conservation from Scripps Institution of Oceanography, and co-founder and CTO Michelle Wolf holds a bachelor’s in materials science and engineering and a master’s in biomedical engineering. New Wave Foods’ first products will reach consumers as early as Q4 2016.
… after trying a lab-made “shrimp” made of plant proteins and algae, I’d consider giving it up the real thing. Maybe others will too.
The shrimp I ate came from New Wave Foods, a startup that just graduated from biotech startup accelerator IndieBio. When I first met New Wave’s founders in the fall of 2015, they had been working for eight weeks at IndieBio’s San Francisco lab. …
Barnes and Wolf [marine conservationist Dominique Barnes and materials scientist Michelle Wolf ] ultimately figured out a way to use plant proteins, along with the same algae that shrimp eat — the stuff that helps give the crustaceans their color and flavor — to come up with a substitute that has a similar texture, taste, color, and nutritional value.
The fact that New Wave’s product has the same high protein, low fat content as real shrimp is a big source of differentiation from other shrimp substitutes, according to Barnes.
In early February, I finally tried a breaded version of New Wave’s shrimp. Here’s what it looked like:
Ariel Schwartz/Tech Insider
It was a little hard to judge the taste because of the breading, but the texture was almost perfect. The lab-made shrimp had that springiness and mixture of crunch and chew that you’d expect from the real thing. I could see myself replacing real shrimp with this in some situations.
Whether it could replace shrimp all the time depends on how the product tastes without the breading. “Our ultimate goal is to get to the cocktail shrimp level,” says Barnes.
I’m glad to have stumbled across Ariel Schwartz again as I’ve always enjoyed her writing and it has been a few years.
One final comment, I am not endorsing this company or its products. This is presented as interesting information and, hopefully, I will be hearing more about the company and its products in the future.
One-third to one-half of the researchers getting grants are working on nanotechnology projects. From a March 1, 2016 University of British Columbia (UBC) news release (received via email),
Research into forest renewal, quantum computer nanotechnology, solar power, high-tech manufacturing, forestry products and the Subarctic ocean climate gained a boost today, with the announcement of $3.5 million in funding for six UBC projects from the Natural Sciences and Engineering Research Council of Canada (NSERC).
The funding comes from NSERC’s Strategic Partnership Grants, which support scientific partnerships to strengthen the Canadian economy, society and environment.
Konrad Walus, Associate Professor, Department of Electrical and Computer Engineering
A framework for embedding, simulation and design of computational nanotechnology using a quantum annealing processor [emphasis mine] — $394,500
This project will work with Quantum Silicon Inc. [emphasis mine] to conduct experiments that provide better insight into the potential of quantum computing, and will develop design rules for future designers of the technology.
Alireza Nojeh, Professor, Department of Electrical and Computer Engineering
Thermionic solar energy converter — $510,500
In close collaboration with four Canadian industrial partners, this project will establish a novel approach to solar electricity generation using recent discoveries in nanostructured materials.
This project will investigate novel forest renewal methods, and establish recommendations for best harvesting and regeneration practices under changing climate conditions.
Chadwick Sinclair, Professor, Faculty of Applied Science – Materials Engineering
Through-process modeling for optimized electron beam additive manufacturing — $484,400
Working in collaboration with Canadian electron-beam processor PAVAC Industries Inc. [emphasis mine], this project will develop a through-process model for additive manufacturing that will link machine control to material microstructure and properties.
Philippe Tortell, Professor, Department of Earth, Ocean and Atmospheric Sciences
Quantifying climate-dependent and anthropogenic impacts on ecosystem services in the Subarctic Pacific Ocean; State-of-the-art observational tools to inform policy and management — $707,100
University scientists and Fisheries and Oceans Canada will use field-based observations to generate satellite-based models of ecosystem productivity to examine fish yields and environmental variability.
The vast open ocean presents an especially challenging environment for its inhabitants since there is nowhere for them to hide. Yet, nature has found a remarkable way for fish to hide from their predators using camouflage techniques. In a study published in the current issue of Science, researchers from Harbor Branch Oceanographic Institute at Florida Atlantic University and collaborators show that fish scales have evolved to not only reflect light, but to also scramble polarization. They identified the tissue structure that fish evolved to do this, which could be an analog to develop new materials to help hide objects in the water.
HBOI researchers and colleagues collected more than 1,500 video-polarimetry measurements from live fish from distinct habitats under a variety of viewing conditions, and have revealed for the first time that fish have an ‘omnidirectional’ solution they use to camouflage themselves, demonstrating a new form of camouflage in nature — light polarization matching.
“We’ve known that open water fish have silvery scales for skin that reflect light from above so the reflected intensity is comparable to the background intensity when looking up, obliquely at the fish, as a predator would,” said Michael Twardowski, Ph.D., research professor at FAU’s HBOI and co-author of the study who collaborated with co-author James M. Sullivan, Ph.D., also a research professor at FAU’s HBOI. “This is one form of camouflage in the ocean.”
Typical light coloring on the ventral side (belly) and dark coloring on the dorsal (top) side of the fish also can help match intensity by differential absorption of light, in addition to reflection matching.
Light-scattering processes in the open ocean create spatially heterogeneous backgrounds. Polarization (the directional vibration of light waves) generates changes in the light environment that vary with the Sun’s position in the sky.
Polarization is a fundamental property of light, like color, but human eyes do not have the ability to sense it. Light travels in waves, and for natural sunlight, the direction of these waves is random around a central viewing axis. But when light reflects off a surface, waves parallel to that surface are dominant in the reflected beam. Many visual systems for fish have the ability to discriminate polarization, like built-in polarized sunglasses.
“Polarized sunglasses help you see better by blocking horizontal waves to reduce bright reflections,” said Twardowski. “The same principle helps fish discriminate objects better in water.”
Twardowski believes that even though light reflecting off silvery scales does a good job matching intensity of the background, if the scales acted as simple mirrors they would impart a polarization signature to the reflected light very different from the more random polarization of the background light field.
“This signature would be easily apparent to a predator with ability to discriminate polarization, resulting in poor camouflage,” he said. “Fish have evolved a solution to this potential vulnerability.”
To empirically determine whether open-ocean fish have evolved a cryptic reflectance strategy for their heterogeneous polarized environments, the researchers measured the contrasts of live open-ocean and coastal fish against the pelagic background in the Florida Keys and Curaçao. They used a single 360 degree camera around the horizontal plane of the targets and used both light microscopy and full-body video-polarimetry.
The American Association for the Advancement of Science (AAAS), publisher of Science magazine where the researchers’ study can be found issued a Nov. 19, 2015 news release on EurekAlert further describing the work,
… The study’s insights could pave the way to improvements in materials like polarization-sensitive satellites. Underwater, light vibrates in way that “polarizes” it. While humans cannot detect this vibrational state of light without technology, it is becoming increasingly evident that many species of fish can; lab-based studies hint that some fish have even adapted ways to use polarization to their advantage, including developing platelets within their skin that reflect and manipulate polarized light so the fish are camouflaged. To gain more insights into this form of camouflage, Parrish Brady and colleagues measured the polarization abilities of live fish as they swam in the open ocean. Using a specialized underwater camera (…), the researchers took numerous polarization measurements of several open water and coastal species of fish throughout the day as the sun changed position in the sky, causing subsequent changes in the polarization of light underwater. They found that open water fish from the Carangidae fish family, such as lookdowns and bigeye scad, exhibited significantly lower polarization contrast with their backgrounds (making them harder to spot) than carangid species that normally inhabit reefs. Furthermore, the researchers found that this reflective camouflage was optimal at angles from which predators most often spot fish, such as from directly below the fish and at angles perpendicular to their length. By looking at the platelets of open water fish under the microscope, the team found that the platelets align well on vertical axes, allowing fish to reflect the predictable downward direction of light in the open ocean. Yet the platelets are angled in way that diffuses light along the horizontal axis, the researchers say. They suggest that these different axes work together to reflect a wide range of depolarized light, offering better camouflage abilities to their hosts.
The AAAS has made available a video combining recordings from the researchers and animation to illustrate the research,
Be sure you can hear the audio as this won’t make much sense otherwise.
Here’s a link to and a citation for the paper,
Open-ocean fish reveal an omnidirectional solution to camouflage in polarized environments by Parrish C. Brady, Alexander A. Gilerson, George W. Kattawar, James M. Sullivan, Michael S. Twardowski, Heidi M. Dierssen, Meng Gao, Kort Travis, Robert Ian Etheredge, Alberto Tonizzo, Amir Ibrahim, Carlos Carrizo, Yalong Gu, Brandon J. Russell, Kathryn Mislinski, Shulei Zha1, Molly E. Cummings. Science 20 November 2015: Vol. 350 no. 6263 pp. 965-969 DOI: 10.1126/science.aad5284